Gibco opti mem 1 glutamax 1 reduced serum medium liquid

RNA interference experiments were performed using Lipofectamine RNAi Max (Invitrogen) in GIBCO^™ Opti-MEM I (GlutaMAX^™-I) Reduced-Serum Medium liquid (Invitrogen) and 20 pmol/ml siRNA. The siGADD34 oligonucleotides were purchased from ThermoFisher (HSS177543). 200 000 HEK293T cells were incubated at 37°C in a CO₂ incubator in antiobiotic free medium for 16 hours, then the RNAi duplex-Lipofectamine^™ RNAiMAX complexes were added to the cells for overnight. Then fresh medium was added to the cells and the appropriate treatment was carried out.

RNA interference experiments were performed using Lipofectamine RNAi Max (Invitrogen, Carlsbad, CA, USA) in GIBCO™ Opti-MEM I (GlutaMAX™-I) Reduced-Serum Medium liquid (Invitrogen) and 20 pmol/mL siRNA. The siPERK oligonucleotides were purchased from Eurofins Genomics (the oligonucleotide sequence: 5′-GUGACGAAAUGGAACAAGA(dTdT)-3′). A total of 200,000 HEK293T cells were incubated at 37 °C in a CO₂ incubator in antibiotic-free medium for 42 h, then the RNAi duplex-Lipofectamine™ RNAiMAX complexes were added to the cells for 24 h. Then fresh medium was added to the cells and the appropriate treatment was carried out.

RNA interference experiments were performed using Lipofectamine RNAi Max (Invitrogen Thermo Fisher Scientific Inc., Waltham, MA, USA) in GIBCO™ Opti-MEM I (GlutaMAX™-I) Reduced-Serum Medium liquid (Invitrogen) and 20 pmol/mL siRNA. The siTSC1 and siTSC2 oligonucleotides were purchased from Ambion (Ambion Thermo Fisher Scientific Inc., Waltham, MA, USA, 138713, 138741) and the siULK oligonucleotides were purchased from Ambion (118259). A total of 200,000 HEK293T cells were incubated at 37 °C in a CO₂ incubator in an antiobiotic free medium for 16 h, then the RNAi duplex-Lipofectamine™ RNAiMAX (Invitrogen, 13778-075) complexes were added to the cells overnight. Then, fresh medium was added to the cells and the appropriate treatment was carried out.