24 well plate

Manufactured by Greiner

Sourced in Germany, Austria, United States, United Kingdom, Switzerland, Belgium

The 24-well plates are a laboratory equipment designed for cell culture, tissue culture, and other biological experiments. They provide a standardized format with 24 individual wells, allowing for multiple samples or conditions to be tested simultaneously. The plates are typically made of high-quality, inert materials to ensure compatibility with a variety of cell types and experimental setups.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions) Paraformaldehyde (pfa), by Merck Group (6 mentions) Streptomycin, by Thermo Fisher Scientific (5 mentions) Penicillin, by Thermo Fisher Scientific (5 mentions) Microscope slide, by Thermo Fisher Scientific (5 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) 96 well plate, by Greiner (4 mentions) Vectashield with dapi, by Vector Laboratories (4 mentions) Autoclaved 12mm circle micro cover glass, by Avantor (4 mentions) Mowiol, by Merck Group (4 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (3 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (3 mentions) Zellshield, by Harvard Bioscience (3 mentions) Spark plate reader, by Tecan (3 mentions) Ham s f 12, by Merck Group (3 mentions) Streptomycin, by Merck Group (3 mentions) Penicillin, by Merck Group (3 mentions) Formaldehyde, by Merck Group (3 mentions) Hek293, by LGC (3 mentions) Pro bind u bottom plate, by BD (3 mentions)

Close spelling variants of this product

24-well cell culture plates (24 citations) 24-well suspension culture plates (5 citations) 24-well microtiter plates (5 citations) 24-well polystyrene plates (3 citations) CELLSTAR® 24-well plates (3 citations) Collagen-coated 24 well plates (3 citations) Sterile 24-well plates (3 citations) Glass-bottom dark 24-well plates (3 citations) Sterile 24-well plastic plates (2 citations) 24-well flat-bottom tissue culture plates (2 citations)

240 protocols using 24 well plate

Live-cell Imaging of HSC Migration

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Micrographs were taken using an Axio Observer Z1 equipped with an Axio Cam MR and an XLmulti S1 DARK LS incubator, providing identical conditions like in a normal incubator. To process data, we used the ZEN pro. 2012 software (Carl Zeiss MicroImaging GmbH, Göttingen, Germany). Pictures were made every 15 minutes. To evaluate the effects of cell culture on HSC proliferation, we normalized the cell number data to the starting point.
To study HSC migration into a defined area, we used culture inserts for self-insertion (IBIDI, Martinsried, Germany) that were inserted before adding the cells to 24-well plates (Greiner, Kremsmünster, Austria). Cells grow among these inserts and, to initiate the migration experiment, the inserts were removed and cells began to move to the empty space (similar to a “scratch assay,” but with a well-defined area for the cells to migrate and as a big advantage, no cells are harmed in this assay).

Bartneck M., Warzecha K.T., Tag C.G., Sauer-Lehnen S., Heymann F., Trautwein C., Weiskirchen R, & Tacke F. (2015). Isolation and Time Lapse Microscopy of Highly Pure Hepatic Stellate Cells. Analytical Cellular Pathology (Amsterdam), 2015, 417023.

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Cell Counting and Culture Protocols

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Cells were counted using a hemocytometer (Neubauer chamber) and 0.4% trypan blue (Sigma-Aldrich, St. Louis, MO, USA). The purity after sorting was determined by a BD FACS Aria II SORP Cell Sorter (BD Biosciences, Franklin Lakes, NJ, USA) and a hemocytometer. The cell pellet was suspended in DMEM cell culture medium supplemented with 25 mM HEPES, 4.5 g/L glucose, 4 mM glutamine, 1% penicillin/streptomycin, and 10% FBS (all Lonza, Basel, Switzerland). Cell culture was done in 24-well plates (Greiner, Kremsmünster, Austria) using 40,000 cells per well in a volume of 1 mL medium.

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HMEC-1 Cell Growth Assay under Hypoxia

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HMEC-1 cells or HMEC-1 cells overexpressing xCT or SLC1A3 were seeded into 24-well plates (Greiner Bio-One) at a density of 6.5 x 10 3 cells per well in 0.5 mL medium. The following day, medium was changed to 1 mL HMEC-1 growth medium with any indicated supplements. For assays with added aspartate, medium was changed to custom DMEM medium without glucose, glutamine, or pantothenate (US Biological) supplemented with 5.55 mM glucose (Gibco), 1 mM pyruvate (Sigma), and 25 µM pantothenate (Sigma) along with supplements above: EGF, hydrocortisone, glutamine, HI-FBS. Media was buffered with sodium hydroxide to pH 7.95.
Designated plates were placed in hypoxia chamber at 1% oxygen. Cell number was measured at the indicated time points using sulforhodamine B (Sigma) staining, as previously described (42) .
Absorbance at 510 nm was read on an Epoch plate reader (BioTek). Relative growth was determined as (treatment absorbance / control absorbance).

Cohen E.B., Geck R.C., & Toker A. (2020). Metabolic pathway alterations in microvascular endothelial cells in response to hypoxia.

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MCF-7 Cell Culture in DMEM Media

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MCF-7 cells were incubated in a 25 cm³ flask in liquid DMEM media supplemented with 1% HEPES buffer media, 1% streptomycin and 10% FBS. The flask was incubated in 37 °C and 5% CO₂. MCF-7 cells in DMEM were seeded into 24 well plates (Greiner, Germany). 50,000 cells were seeded per well. Estimations of cell counts were made using a Hirschmann counting chamber (haemocytometer). The wells were incubated in 37 °C and 5% CO₂ for 24 h.

Al-Busaidi H., Karim M.E., Abidin S.A., Tha K.K, & Chowdhury E.H. (2019). Magnesium Fluoride Forms Unique Protein Corona for Efficient Delivery of Doxorubicin into Breast Cancer Cells. Toxics, 7(1), 10.

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Isolate and Expand Activated T Cells

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Isolated islets pooled from eight rats were cultured with interleukin (IL)-2 to promote the expansion of activated T cells. Culture medium contained RPMI 1640 with 10% FBS, 1 mmol/L Na pyruvate, nonessential amino acids, 28 μmol/L β-mercaptoethanol, and 50 units/mL recombinant human IL-2 (PeproTech, Rocky Hill, NJ). Fifty islets per well were cultured in 24-well plates (Greiner Bio-One) in 5% CO₂ at 37°C. On day 7, islets and infiltrating cells were collected and passed through a 40-μm strainer to retain the islets. T cells were pelleted and resuspended in TRIzol (28 (link)).

Eberwine R.A., Cort L., Habib M., Mordes J.P, & Blankenhorn E.P. (2014). Autoantigen-Induced Focusing of Vβ13+ T Cells Precedes Onset of Autoimmune Diabetes in the LEW.1WR1 Rat. Diabetes, 63(2), 596-604.

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Transient CRISPR/TALE Transfection in HEK293T and Fibroblasts

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Human HEK293T and Rabbit fibroblasts cells were cultured in Dulbecco’s modified Eagle’s medium (HyClone) supplemented with 10% fetal bovine serum (Gibco) at 37°C under 5% CO₂. Then, they were seeded on 24-well plates (Greiner Bio-One) and transfected at approximately 90% density. After 24 h, 250 ng of sgRNA, 500 ng of plasmids with nCas9, and 300 ng of plasmids with TALE were transfected using 1 μl of Lipo8000 Transfection Reagent (Beyotime, C0533) per well. After 3 days of transfection, cells were collected and filtered through a 40 μm strainer (BD Falcon), and the transfected cells were collected on a Beckman Coulter MoFlo Astrios Cell Sorter (laser option: blue 488 nm and red 561 nm). About 10 μl of NP40 Lysis Buffer was added to the collected cells, and the reaction was incubated at 56°C for 1 h. The products were used as a template for amplification.

Zhou J., Wang J., Chen F., Zhuang Z., Chen M., Yang Y., Luo X., Tang C., Zhou X., Chi Y., Wang J., He Y., Zhang K, & Zou Q. (2023). Improved USER cloning for TALE assembly and its application to base editing. PLOS ONE, 18(8), e0289509.

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Monocyte Adhesion to HUVEC Monolayers

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Monolayers of HUVEC were grown to confluence in 24-well plates (Greiner Bio-One). HUVECs were stimulated with macrophage supernatant as above, or with TNFα (10 ng/ml) for 24 h and then suspended in fresh media before experiments. 1 × 10⁵ monocytes were added per chamber for 30 min. After 30 min, non-adherent cells were washed away, and the cells were fixed using 4% formaldehyde in PBS and blocked in 5% BSA in PBS for 1 h. The cells were the stained with mouse anti-human CD14 FITC conjugated or mouse IgG isotype control (both 1:100) (both ImmunoTools). The cells were visualised using the EVOS XL microscope, and the number of adhered cells counted using ImageJ software.

Bridgewood C., Fearnley G.W., Berekmeri A., Laws P., Macleod T., Ponnambalam S., Stacey M., Graham A, & Wittmann M. (2018). IL-36γ Is a Strong Inducer of IL-23 in Psoriatic Cells and Activates Angiogenesis. Frontiers in Immunology, 9, 200.

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Transparency Measurements of PLGA-loaded BNC Fleeces

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Transparency measurements were performed as described before [41 (link)], using the Tecan 20M multiplate spectrophotometer (Tecan Group AG, Maennedorf, Switzerland). PLGA-loaded and native BNC fleeces with a similar height were placed in 24-well plates (Greiner bio-one GmbH, Frickenhausen, Germany) and measured at a wavelength of 600 nm. The mean of the transmission of five equally distributed measurement points was calculated for each BNC fleece using the SparkControl™ 2.2. software (Tecan Group). Experiments were performed in triplicates and repeated once.

Bellmann T., Thamm J., Beekmann U., Kralisch D, & Fischer D. (2023). In situ Formation of Polymer Microparticles in Bacterial Nanocellulose Using Alternative and Sustainable Solvents to Incorporate Lipophilic Drugs. Pharmaceutics, 15(2), 559.

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Evaluation of FimH and MrpH Bioactivity in HT-29 Cells

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The HT-29 cell line was purchased from the Cell Bank, Pasteur Institute of Iran. The HT-29 cell line is a human colorectal epithelial cell line that expresses TLR4. The cell line was used to test the bioactivity of purified FimH and the fusion protein MrpH. FimH. The TLR4 activity of the proteins was evaluated based on the induction of IL-8. Briefly, HT-29 cells were cultured in 24-well plates (Greiner, Germany) at a density of 5 × 10⁴ cells/well in 1 ml fresh Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Biosera, North America) and antibiotics (Biosera, North America). After overnight incubation, the cells were incubated for 5 h with 10 μg/ml of sterilized of FimH and fusion MrpH. FimH. Then, supernatants were collected and the expression of IL-8 was evaluated using the enzyme-linked immunosorbent assay (ELISA) (R and D systems, USA).

Habibi M., Asadi Karam M.R, & Bouzari S. (2015). In silico design of fusion protein of FimH from uropathogenic Escherichia coli and MrpH from Proteus mirabilis against urinary tract infections. Advanced Biomedical Research, 4, 217.

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Isolation and Culture of Human Myometrial Cells

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Human myometrial tissue was enzymatically dispersed into single cells in serum-free DMEM (Invitrogen, Paisley, UK) containing collagenase type II (300 U/ml; Invitrogen), dispase (0.3 U/ml; Invitrogen), DNase (30 U/ml; Sigma Aldrich, Poole, UK) and elastase (0.09 U/ml; Sigma Aldrich) at 37°C for 4.5 h with shaking. Liberated cells were cultured in T75 flask in DMEM supplemented with 10% (v/v) fetal calf serum (FCS; Invitrogen), 100 U/ml penicillin (Invitrogen) and 100 μg/ml streptomycin (Invitrogen) at 37°C and 5% CO₂. Cells were seeded at 4 × 10⁴ cells/well in 24-well plates (Greiner Bio-One Ltd, Stonehouse, Gloucestershire, UK) and grown to confluence over 3 days. Cultured primary myometrial cells were used between passages 2–10.

Wouters E., Hudson C.A., McArdle C.A, & López Bernal A. (2014). Central role for protein kinase C in oxytocin and epidermal growth factor stimulated cyclooxygenase 2 expression in human myometrial cells. BMC Research Notes, 7, 357.

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