Log In Sign up
The largest database of trusted experimental protocols

Marcks

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

MARCKS is a laboratory reagent used in cell biology research. It functions as a substrate for protein kinase C (PKC) and plays a role in cell signaling pathways.

Automatically generated - may contain errors

Lab products found in correlation

β actin, by Cell Signaling Technology (2 mentions) P erk, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Odyssey secondary antibodies, by LI COR (1 mentions) P fak, by Cell Signaling Technology (1 mentions) β actin, by Abcam (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Crystal violet, by Merck Group (1 mentions) Trypan blue solution, by Merck Group (1 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions) G 6976, by Merck Group (1 mentions) Cep 1347, by Bio-Techne (1 mentions) Phospho pkcδ thr505, by Cell Signaling Technology (1 mentions) Phospho pkc pan βii ser660, by Cell Signaling Technology (1 mentions) P53 antibody sc 126, by Santa Cruz Biotechnology (1 mentions) Af1244, by R&D Systems (1 mentions) Phospho pkcα β 2 thr638 641, by Cell Signaling Technology (1 mentions) Triton x 100, by Merck Group (1 mentions) Hank s balanced salt solution hbss, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-MARCKS (3 citations) Phospho-MARCKS (3 citations) Anti-MARCKS antibody (2 citations) Anti-Phospho-MARCKS (2 citations)

6 protocols using marcks

1

Protein Expression Analysis of Drug Combinations

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were cultured in 10 cm-diameter dishes and treated with DMSO or each drug alone or in combination for 1 and 2 h, or for 3 days and 5 days, at synergistic doses. After separation by SDS-PAGE and transferring onto polyvinylidene difluoride (PVDF) mem-branes, protein extracts were probed with antibodies against pFAK (#8556), FAK (#3285), pMARCKS (#11992), MARCKS (#5607), pERK (#9101), ERK (#9102), cleaved and total PARP (#9542), and GAPDH (#97166), all purchased from Cell Signaling Technology, Danvers, MA, USA. GAPDH immunoblotting was used to quantify and normalize results. Immunolabelled proteins were detected using Odyssey secondary antibodies coupled to a 700 or 800 nm and the Odyssey Infrared Imaging System (Li-cor).
Tarin M., Némati F., Decaudin D., Canbezdi C., Marande B., Silva L., Derrien H., Jochemsen A.G., Gardrat S., Piperno-Neumann S., Rodrigues M., Mariani P., Cassoux N., Stern M.H., Roman-Roman S, & Alsafadi S. (2023). FAK Inhibitor-Based Combinations with MEK or PKC Inhibitors Trigger Synergistic Antitumor Effects in Uveal Melanoma. Cancers, 15(8), 2280.
+ Open protocol
+ Expand
2

Protein Expression Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in Triton X-sample buffer and separated by SDS-PAGE. Proteins were detected using specific antibodies directed against β-actin (BioVision via BioCat GmbH, Heidelberg, Germany), PKCα (abcam, Cambridge, UK), PKCβ (BD Biosciences, Heidelberg, Germany), MARCKS (Cell Signaling Technology, Danvers, MA), and phosphorylated MARCKS (Cell Signaling Technology). Protein bands were visualised by enhanced chemiluminescence using a commercially available kit (Amersham, Freiburg, Germany).
Michaelis M., Rothweiler F., Löschmann N., Sharifi M., Ghafourian T, & Cinatl J J.r. (2015). Enzastaurin inhibits ABCB1-mediated drug efflux independently of effects on protein kinase C signalling and the cellular p53 status. Oncotarget, 6(19), 17605-17620.
+ Open protocol
+ Expand
3

Molecular Signaling Pathway Inhibitors

Check if the same lab product or an alternative is used in the 5 most similar protocols
CEP-1347 was purchased from TOCRIS Bioscience (Bristol, UK). Gö6976, Crystal Violet (C.I. 42555), and Trypan Blue solution (0.4%) were obtained from Merck KGaA (Darmstadt, Germany). Propidium iodide and Hoechst33342 were supplied by Thermo Fisher Scientific (Waltham, MA, USA). An antibody against MDM4 (A700-000-T) was purchased from BETHYL (FORTIS LIFE SCIENCES, Waltham, MA, USA). An antibody against MDM2 (AF1244) was purchased from R&D Systems (Minneapolis, MN, USA). A p53 antibody (sc-126) was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Antibodies against GAPDH (#5174), p21 Waf1/Cip1 (cyclin-dependent kinase inhibitor 1A, CDKN1A) (#2947), Myristoylated alanine-rich protein kinase C (PKC) substrate (MARCKS) (#5607), Phospho-MARCKS (Ser152/156) (#2741), Phospho-PKC (pan) (βII Ser660) (#9371), Phospho-PKCα/β II (Thr638/641) (#9375), Phospho-PKCδ (Thr505) (#9374), and Phospho-PKCθ (Thr538) (#9377) were from Cell Signaling Technology, Inc. (Beverly, MA, USA).
Togashi K., Suzuki S., Mitobe Y., Nakagawa-Saito Y., Sugai A., Takenouchi S., Sugimoto M., Kitanaka C, & Okada M. (2023). CEP-1347 Dually Targets MDM4 and PKC to Activate p53 and Inhibit the Growth of Uveal Melanoma Cells. Cancers, 16(1), 118.
+ Open protocol
+ Expand
4

Neutrophil Activation Assays Utilizing Diverse Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ficoll-Paque Plus and Dextran T500 were from GE Healthcare (Sweden). Dimethyl sulfoxide (Me2SO), f-Met-Leu-Phe (fMLF), phorbol 12-myristate 13-acetate (PMA), Triton-X 100, pepstatin, HEPES, staurosporine, Protein kinase C-ζ pseudosubstrate and poly-l-lysine were from Sigma Chemical Co. (St. Louis, MO). Rottlerin, Gö6976 and CG53353 were from Calbiochem (Billerica, MA). Powdered phosphate-buffered saline (PBS) and Hank’s balanced salt solution (HBSS) were from Life Technologies (Grand Island, NY). Ethylenediamine tetraacetate dihydrate (EDTA) was from Fisher Scientific (Atlanta, GA). Leukotriene B4 (LTB4) was from Cayman Chemical (Ann Arbor, MI). Interleukin 8 (IL-8) was from Sigma-Aldrich Co. LLC (St. Louis, MO, USA). Fetal calf serum (FCS) was obtained from Hyclone (Logan, UT). MARCKS, Phospho-MARCKS, PKC α, PKC δ, PKC ζ primary antibodies and anti-rabbit HRP-conjugated secondary antibody were obtained from Cell Signaling (Beverly, MA). PKC β was from Invitrogen (Frederic, MD). Diisopropylfluorophosphate (DFP) was from BD Biosciences (San Diego, CA).
Sheats M.K., Sung E.J., Adler K.B, & Jones S.L. (2015). In vitro neutrophil migration requires protein kinase c-delta (δ-PKC) mediated MARCKS (Myristoylated Alanine Rich C-Kinase Substrate) phosphorylation. Inflammation, 38(3), 1126-1141.
+ Open protocol
+ Expand
5

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies for STAT3, p-STAT3-S727, p-STAT3-Y705, Mcl-1, Ran, STAT1, p-STAT1-Y701, p-STAT1-S727, STAT2, p-STAT2-Y690, STAT5, Akt-S473, BTK, p-BTK-Y223, p-ERK (T202/Y204), ERK, p-MARCKS (Ser 152/156), MARCKS, survivin, XIAP, cyclin D1, p21 and β-actin were purchased from Cell Signaling Technology Inc (Danvers, MA, USA). The anti-FLAG antibody was purchased from Sigma (St Louis, MO, USA). For western blotting, precasted Nupage electrophoresis gels were purchased from Invitrogen (Carlsbad, CA, USA) and chemiluminescence reagent was obtained from Thermo Scientific (Waltham, MA, USA). STAT3 inhibitor, Stattic; MEK inhibitor, U0126 and PKC inhibitor, Bis-I were purchased from Sigma. BTK inhibitor, ibrutinib, was purchased from MedChem Express (Monmouth Junction, NJ, USA).
Doshi U.A., Shaw J., Fox T.E., Claxton D.F., Loughran T.P, & Kester M. (2017). STAT3 mediates C6-ceramide-induced cell death in chronic lymphocytic leukemia. Signal Transduction and Targeted Therapy, 2, 17051-.
+ Open protocol
+ Expand
6

Reagents and Antibodies Utilized

Check if the same lab product or an alternative is used in the 5 most similar protocols
Enmein was purchased from Tauto Biotech (Shanghai, China) at 98% purity. 4-AP, bafilomycin A1, GF109203X and Go6976 were purchased from Tocris Cookson (Bristol, UK). Fura-2-AM was purchased from Invitrogen (Carlsbad, CA, USA). ω-CgTX GVIA and ω-AgTX IVA were purchased from the Alomone lab (Jerusalem, Israel). PKC, PKC-α, MARCKS, EAAT3, synaptophysin, GFAP and β-actin antibodies were obtained from Cell Signaling (Beverly, MA, USA). EAAT2, PSD95, CD11b p-PKC and p-PKC-α, antibodies were obtained from Abcam (Cambridge, UK). NeuN antibody was obtained from Merck Millipore (Billerica, MA, USA). Horseradish peroxidase-conjugated secondary antibodies were obtained from Genetex (Zeeland, MI, USA). Alexa Fluor 488 and DyLight 594 were obtained from Invitrogen (Waltham, MA, USA). KA, DMSO, Cresyl violet, 4′,6-diamidino-2-phenylindole (DAPI) and general reagents were purchased from Sigma Chemical Co. (St Louis, MO, USA).
Lu C.W., Huang Y.C., Chiu K.M., Lee M.Y., Lin T.Y, & Wang S.J. (2021). Enmein Decreases Synaptic Glutamate Release and Protects against Kainic Acid-Induced Brain Injury in Rats. International Journal of Molecular Sciences, 22(23), 12966.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!