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Phenylephrine hydrochloride

Manufactured by Merck Group
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Phenylephrine hydrochloride is a chemical compound commonly used as a laboratory reagent. It is a crystalline powder that is soluble in water and alcohol. Phenylephrine hydrochloride is primarily used in various analytical and research applications, but its specific functions and intended uses are not provided in this factual and unbiased description.

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Lab products found in correlation

Acetylcholine chloride, by Merck Group (21 mentions) Sodium nitroprusside, by Merck Group (10 mentions) Acetylcholine, by Merck Group (9 mentions) L name, by Merck Group (8 mentions) Indomethacin, by Merck Group (8 mentions) Nω nitro l arginine methyl ester l name, by Merck Group (6 mentions) Acetylcholine hydrochloride, by Merck Group (6 mentions) Dimethyl sulfoxide (dmso), by Merck Group (5 mentions) Nω nitro l arginine methyl ester hydrochloride l name, by Merck Group (4 mentions) Isoprenaline hydrochloride, by Merck Group (4 mentions) Propranolol hydrochloride, by Merck Group (4 mentions) Atropine sulfate, by Merck Group (4 mentions) Isoproterenol hydrochloride, by Merck Group (4 mentions) Prazosin hydrochloride, by Merck Group (4 mentions) Tyramine hydrochloride, by Merck Group (3 mentions) Glibenclamide, by Merck Group (3 mentions) Atenolol, by Merck Group (3 mentions) Tribromoethanol, by Merck Group (3 mentions) Urethane, by Merck Group (3 mentions) Papaverine hydrochloride, by Merck Group (3 mentions)

Close spelling variants of this product

Phenylephrine hydrochloride (PE), ).(R)-(−)-phenylephrine hydrochloride (P6126) L-phenylephrine hydrochloride (R)-(−)-phenylephrine hydrochloride (PE), Phenylephrine hydrochloride (PHE) (R)-(−)-phenylephrine hydrochloride Phenylephrine Hydrochlorides

82 protocols using phenylephrine hydrochloride

1

Cardiac Hypertrophy and Fibrosis Induction

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Adult mice >20.0 g were infused with AngII/PE to induce cardiac hypertrophy and fibrosis. Mice were anesthetized with 1–2% isoflurane and mini-osmotic pumps (Alzet, 2001, 2002, or 2004) were implanted subcutaneously. A combination of 1.5 μg/g/day angiotensin II (Calbiochem, 05-23-0101) and 50 μg/g/day phenylephrine hydrochloride (Sigma, P6126) or saline, was infused for 7, 14, or 28 days.
Kuwabara J.T., Hara A., Bhutada S., Gojanovich G.S., Chen J., Hokutan K., Shettigar V., Lee A.Y., DeAngelo L.P., Heckl J.R., Jahansooz J.R., Tacdol D.K., Ziolo M.T., Apte S.S, & Tallquist M.D. (2022). Consequences of PDGFRα+ fibroblast reduction in adult murine hearts. eLife, 11, e69854.
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2

CD4+ T cell polarization protocol

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Purified CD4 T cells were stimulated with 10μg/ml plate bound anti-CD3ε (clone 145-2C11) and 2μg/ml soluble anti-CD28 (37.51) in RPMI 1640 as before in flat bottom 96 well plates. For Th1 polarisation, cells were also treated with 15ng/ml recombinant mouse IL-12 and 5μg/ml anti-IL-4 (11B11), or for Th2 polarisation, 30ng/ml recombinant mouse IL-4 and 5μg/ml anti-IFNγ (XMG1.2). Phenylephrine hydrochloride (Sigma) was used at 10μM and added during both anti-CD3 dependent activation (4 days) and also during rest in 10U/ml recombinant human IL-2 (2 days). All antibodies were from Biolegend and were low endotoxin / azide free, and recombinant cytokines were from Peprotech.
Hewitson J.P., Shah K.M., Brown N., Grevitt P., Hain S., Newling K., Sharp T.V., Kaye P.M, & Lagos D. (2019). miR‐132 suppresses transcription of ribosomal proteins to promote protective Th1 immunity. EMBO Reports, 20(4), e46620.
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3

Pharmacological Modulation of Vascular Tone

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Etomidate was purchased from Nhwa (Nhwa Pharmaceutical, Jiangsu, CHN). Intralipid was purchased from SSPC (Sino-Swed Pharmaceutical, Jiangsu, CHN). Phenylephrine hydrochloride (PE), KCl, acetylcholine chloride (ACh), sodium nitroprusside (SNP), L-NAME, indomethacin (Indo), iberiotoxin, 4-aminopyridine (4-AP) and glibenclamide (Gli) were purchased from Sigma (St. Louis, MO, USA). Gli were dissolved in ethanol (final concentration of ethanol in a tissue bath ≤ 0.5%, with no influence on PE-induced contraction). Phenylephrine hydrochloride, etomidate, ACh, SNP, 4-AP and L-NAME were dissolved in distilled water. Indo was dissolved in 4% (wt/vol) NaHCO3.
Xue W., Li Y., Li J., Yan L, & Yang F. (2018). Endothelium-dependent relaxation induced by etomidate in the aortas of insulin-resistant rats. Archives of Medical Science : AMS, 14(5), 1155-1162.
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4

Measuring Oxidative Stress Markers

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Acetylcholine chloride, phenylephrine hydrochloride, L-NAME, and DAF-2 were purchased from Sigma-Aldrich CO (Saint Louis, MO, USA). MnTMPyP was purchased from Calbiochem (Merck Millipore). DHE was purchased from Invitrogen (Grand Island, NY, USA).
Victorio J.A., Fontes M.T., Rossoni L.V, & Davel A.P. (2016). Different Anti-Contractile Function and Nitric Oxide Production of Thoracic and Abdominal Perivascular Adipose Tissues. Frontiers in Physiology, 7, 295.
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5

Neurochemical Reagent Procurement

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Acetylcholine chloride, nisoxetine hydrochloride, norepinephrine hydrochloride, pargyline hydrochloride, phenol, phenylephrine hydrochloride and tyramine hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Restini C.B., Ismail A., Kumar R.K., Burnett R., Garver H., Fink G.D, & Watts S.W. (2018). Renal perivascular adipose tissue: form and function. Vascular pharmacology, 106, 37-45.
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6

Vasoactive Compounds Pharmacology Protocol

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Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME), Sodium Chloride, Phenylephrine hydrochloride, Acetylcholine chloride and other unspecified chemicals were from Sigma-Aldrich (France), Captopril Denk 25 were from Denk Pharma GmbH&Co (Germany).
Belemnaba L., Nitiéma M., Ilboudo S., Ouédraogo G.G., Ouédraogo N., Belemlilga M.B., Compaoré S., Ouédraogo S, & Ouédraogo S. (2021). Preclinical Evaluation of the Antihypertensive Effect of an Aqueous Extract of Anogeissus leiocarpa (DC) Guill et Perr. Bark of Trunk in L-NAME-Induced Hypertensive Rat. Journal of Experimental Pharmacology, 13, 739-754.
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7

Vasodilatory Effects of Pharmacological Agents

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Petroleum ether, chloroform, ethyl acetate, methanol, phenylephrine hydrochloride (PE), acetylcholine (ACh), indomethacin, and Nω-nitro-L-arginine methyl ester (L-NAME) were purchased from Sigma-Aldrich, Germany; while sodium chloride (NaCl), potassium chloride (KCl), potassium dihydrogen phosphate (KH2PO4), magnesium sulphate (MgSO4⋅7H2O), glucose, sodium hydrogen carbonate (NaHCO3), and calcium chloride dehydrate (CaCl2⋅H2O) were purchased from R&M Chem., UK. All drugs were freshly prepared in normal saline, except indomethacin in 0.5% (w/v) sodium carbonate, prior to use.
Manshor N.M., Razali N., Jusoh R.R., Asmawi M.Z., Mohamed N., Zainol S., Altaf R, & Dewa A. (2020). Vasorelaxant effect of water fraction of Labisia Pumila and its mechanisms in spontaneously hypertensive rats aortic ring preparation. International Journal of Cardiology Hypertension, 4, 100024.
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8

Vitamin D Deficiency Diet Impacts Vascular Function

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A standard chow diet (AIN93G) and a modified vitamin D-deficient diet (SF03-009) were purchased from Specialty Feeds Pty Ltd. (Glen Forrest, Western Australia). The standard diet contains 1000 IU/kg of vitamin D3. Both diets also contained 0.47% calcium and 0.35% phosphorus. Acetylcholine hydrochloride, calcium ionophore, sodium nitroprusside, phenylephrine hydrochloride, and streptozotocin (STZ) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Salbutamol hemisulfate was purchased from Tocris Bioscience (Bristol, UK). L-NAME hydrochloride was obtained from Cayman Chemicals (Ann Arbor, MI, USA). Calcitriol (Rocaltrol) was purchased from Roche (Switzerland). Primary antibody against eNOS (AB76198), mouse monoclonal antibody to β-actin (AB8226), and horseradish peroxidase- (HRP-) conjugated secondary antibody (AB6721) were purchased from Abcam (Cambridge, UK).
Wee C.L., Mokhtar S.S., Singh K.K., Yahaya S., Leung S.W, & Rasool A.H. (2021). Calcitriol Supplementation Ameliorates Microvascular Endothelial Dysfunction in Vitamin D-Deficient Diabetic Rats by Upregulating the Vascular eNOS Protein Expression and Reducing Oxidative Stress. Oxidative Medicine and Cellular Longevity, 2021, 3109294.
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9

Pharmacological Evaluation of Cardiovascular Agents

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Acetylcholine chloride (ACh), verapamil, isoprenaline hydrochloride, carbachol (CCh) and phenylephrine hydrochloride (PE) were purchased from Sigma Chemicals Co. MO, USA. Various kinds of chemical were used for the preparation of physiological solutions like: magnesium sulfate,sodium dihydrogen phosphate, calcium chloride, glucose, sodium bicarbonate ,magnesium chloride and potassium dihydrogen phosphate were purchased from Merck, Germany, while potassium chloride (KCl), ethylenediamine tetraacetic acid from Sigma Chemical Co, USA. Similarly Sodium chloride was purchased from BDH Laboratory, England. Analytical grade chemical were used for performing the experiments.
Khan A.U., Ali S., Gilani A.H., Ahmed M, & Choudhary M.I. (2017). Antispasmodic, bronchodilator, vasorelaxant and cardiosuppressant effects of Buxus papillosa. BMC Complementary and Alternative Medicine, 17, 54.
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10

Baroreflex Testing in Rats

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moving rats, 24 h before the experiment, rats were anesthetized with ketamine (80 Phenylephrine hydrochloride [5 μg/kg of body weight (b. wt.)] and sodium nitroprusside (30 μg/kg of b. wt.) purchased from Sigma Chem. Co. (St. Louis, MO, USA) were injected intravenously (i.v.) for baroreflex testing. The drugs were dissolved in saline.
Speretta G.F., Lemes E.V., Vendramini R.C., Menani J.V., Zoccal D.B., Colombari E., Colombari D.S, & Bassi M. (2018). High-fat diet increases respiratory frequency and abdominal expiratory motor activity during hypercapnia. Respiratory physiology & neurobiology, 258, 32-39.
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