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Axio imager d1 microscope

Manufactured by Zeiss
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Sourced in Germany, Australia

The Axio Imager D1 microscope is a versatile optical microscope designed for a wide range of applications. It features a modular design, allowing for the integration of various accessories and components to suit specific research or industrial needs. The microscope provides high-quality imaging capabilities with advanced illumination and optics systems.

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Lab products found in correlation

Axiocam mrm camera, by Zeiss (3 mentions) Axiocam mrm, by Zeiss (3 mentions) Axiovision, by Zeiss (2 mentions) Ccd camera, by Jenoptik (1 mentions) Isis software, by MetaSystems (1 mentions) Tcs spe confocal microscope, by Leica (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Fastdna spin kit for soil, by MP Biomedicals (1 mentions) Prolong antifade reagent, by Thermo Fisher Scientific (1 mentions) Evolve emccd camera, by Zeiss (1 mentions) Calcein am, by Thermo Fisher Scientific (1 mentions) Axiocammr3 camera, by Zeiss (1 mentions) Axio imager m2 microscope, by Zeiss (1 mentions) Prolong with dapi, by Thermo Fisher Scientific (1 mentions) Cd14 monoclonal antibody clone b365.1 b a8, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Poly l lysin, by Merck Group (1 mentions) Mouse anti mlh1, by Abcam (1 mentions) Orca er camera, by Zeiss (1 mentions) Axioimager z1 microscope, by Hamamatsu Photonics (1 mentions)

Close spelling variants of this product

Axio Imager (671 citations) Axio microscope (122 citations) Axio Imager D1 (30 citations) Imager D1 microscope (8 citations) Axio Imager D1 Upright Fluorescence Microscope (2 citations)

27 protocols using axio imager d1 microscope

1

Imaging Excretory and Somatic Gonad Cells in C. elegans

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arIs164[glt-3p::Venus]56 (link) was used as a marker for the excretory cell. 10–15 epn-1(0); enEx[epn-1::GFP] adults were picked onto a plate seeded with OP50 bacteria, allowed to lay eggs for 3 hours, and then removed. 24 hours later, progeny from epn-1(0); enEx[epn-1::GFP] were picked and imaged on the GFP channel at 50ms exposure time at a Zeiss Axio Imager D1 microscope with an AxioCam MRm at 40x. epn-1(0) progeny were identified by the lack of EPN-1::GFP, which is ubiquitously expressed throughout the animal.
To score lag-2(0) for comparison, lag-2(0) progeny from lag-2(0); arIs164; arEx2511[lag-2(+)] lacking the green pharyngeal marker included in the rescuing array were picked and imaged on the GFP channel at 700ms exposure time on a Zeiss Axio Imager D1 microscope with an AxioCam MRm at 40×. The same was done for the control progeny retaining arEx2511, except images were taken at 50ms exposure time to show presence of green pharynx, since arIs164 is very bright.
Langridge P.D., Garcia-Diaz A., Chan J.Y., Greenwald I, & Struhl G. (2022). Evolutionary plasticity in the requirement for force exerted by ligand endocytosis to activate C. elegans Notch proteins. Current biology : CB, 32(10), 2263-2271.e6.
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2

Decondensation and FISH Analysis of Semen

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The fixed semen sample from brother-2 was spread onto slides, washed in PBS, and incubated in a decondensation solution (10 mM DTT, 100 mM TRIS-HCl; pH 8.5, 43 °C) for 7 min. Next, the slides were rinsed in 2× SSC (pH 7.0), air-dried, and then stored in a freezer at −20 °C until the FISH procedure. Fixed lymphocyte cultures were spread onto slides directly before FISH. FISH was performed following the manufacturer’s protocol (Cytocell, Cambridge, UK) with modifications described previously [57 (link)]. The hybridization mixtures contained various volumes of probes depending on their specificity: centromere—2.0 µL; subtelomere—3.0 µL; wcp/mFISH—10.0 µL; and BAC—5.0 µL. If needed, the mixes were filled with hybridization solution to a final volume of 10 or 20 µL. The FISH efficiency was approximately 98%. For analysis, a Zeiss AxioImager D1 microscope equipped with the necessary filters (DAPI/FITC/SpO/TR/Cy5/DEAC/Triple) and objectives (20×, 100× immersion) was used. Images were acquired with a CCD camera (Jenoptik, Germany) and processed using ISIS software (MetaSystems, Altlussheim, Germany).
Olszewska M., Stokowy T., Pollock N., Huleyuk N., Georgiadis A., Yatsenko S., Zastavna D., Yatsenko A.N, & Kurpisz M. (2020). Familial Infertility (Azoospermia and Cryptozoospermia) in Two Brothers—Carriers of t(1;7) Complex Chromosomal Rearrangement (CCR):  Molecular Cytogenetic Analysis. International Journal of Molecular Sciences, 21(12), 4559.
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3

Microscopy-Based Developmental Staging Protocol

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Unless otherwise specified, we mounted larvae from plates onto 4% agarose pads and immobilized them using 10 mM levamisole. We used the ckb-3p::mCherry-h2b marker to confirm that each scored animal was at the appropriate developmental stage. For assessing AC marker expression and for the primary RNAi screen, animals were scored by eye using a 63x Plan-Apo objective lens on a Zeiss Axio Imager D1 microscope, using an X-Cite 120Q light source for illumination. For all other experiments, a spinning disc confocal equipped with a dual camera system was used to capture images. When both fluorescent proteins were imaged, GFP and mCherry fluorescence were captured simultaneously. A 488-nm, 100-mW laser was used to excite GFP and a 561- nm, 75-mW laser was used to excite mCherry. Exposure times for specific experiments are noted below. Imaging parameters associated with the RNAi screen are described along with other methods pertaining to the screen.
Benavidez J.M., Kim J.H, & Greenwald I. (2022). Influences of HLH-2 stability on anchor cell fate specification during Caenorhabditis elegans gonadogenesis. G3: Genes|Genomes|Genetics, 12(4), jkac028.
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4

Expression Profiling of C. elegans bHLH Genes

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Strains carrying translational reporters were available for 17/18 Class II bHLH genes (all but hlh-32, as no translational reporter was available). The translational reporters contain a large portion of the genomic context of each gene, with all but HLH-16 being fosmid-based [18 (link)]. We examined one transgenic strain for HLH-8, HLH-16, and LIN-32 (integrated), and two independent transgenic strains for the remaining 14 bHLH genes. We visually screened these strains for expression in the hDTC, LC and mDTC in all larval stages (Fig. S1). Males were generated by crossing wild-type N2 or pha-1(e2123) males to hermaphrodite strains carrying fosmid reporter transgenes, except in the case of otIs594[lin-32-gfp fosmid], where the allele him-5(e1490) was used to increase the frequency of male self-progeny.
Expression was examined in more detail for the hlh-2-gfp fosmid (arEx2028), the lin-32-gfp fosmid (otIs594), the hlh-12-gfp fosmid (arIs235) the hlh-8-gfp fosmid (wgIs74), and the hlh-3-gfp fosmid (otIs648), with a minimum of 10 larvae scored for expression at each stage for each reporter (Fig. S1). All scoring was performed at the 63× PlanApo objective on a Zeiss Axio Imager D1 microscope with a Zeiss AxioCam MRM camera, with expression considered “ON” if fluorescence was visible at a 500 ms exposure time for GFP.
Sallee M., Littleford H, & Greenwald I. (2017). A “bHLH code” for sexually dimorphic form and function of the C. elegans somatic gonad. Current biology : CB, 27(12), 1853-1860.e5.
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5

Confocal Imaging and In Situ Hybridization

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Confocal images were obtained with Leica TCS SPE confocal microscope. In situ hybridization results were visualized using DIC optics with Axio Imager D1 microscope (Carl Zeiss). The optical sections were combined in stacks and converted to video files (Supplementary Materials Data S2–S5) by ImageJ. Schemes were made in Adobe Illustrator.
Shalaeva A.Y., Kostyuchenko R.P, & Kozin V.V. (2021). Structural and Functional Characterization of the FGF Signaling Pathway in Regeneration of the Polychaete Worm Alitta virens (Annelida, Errantia). Genes, 12(6), 788.
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6

Single-molecule DNA Fiber Imaging

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Single-molecule DNA fibers were prepared by molecular combing (Michalet et al. 1997 (link)). Purified fosmid DNA was amplified and labeled as described previously (Gribble et al. 2013 (link)). Fluorescence in-situ hybridization followed standard protocols (Supplemental Methods, section 6). Probes were detected with fluorescently conjugated antibodies. Slides were mounted with SlowFade Gold mounting solution containing 4′,6-diamidino-2-phenylindole (Molecular Probes/Invitrogen) and visualized on a Zeiss AxioImager D1 microscope. Digital image capture and processing were carried out using the SmartCapture software (Digital Scientific UK).
Skinner B.M., Sargent C.A., Churcher C., Hunt T., Herrero J., Loveland J.E., Dunn M., Louzada S., Fu B., Chow W., Gilbert J., Austin-Guest S., Beal K., Carvalho-Silva D., Cheng W., Gordon D., Grafham D., Hardy M., Harley J., Hauser H., Howden P., Howe K., Lachani K., Ellis P.J., Kelly D., Kerry G., Kerwin J., Ng B.L., Threadgold G., Wileman T., Wood J.M., Yang F., Harrow J., Affara N.A, & Tyler-Smith C. (2016). The pig X and Y Chromosomes: structure, sequence, and evolution. Genome Research, 26(1), 130-139.
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7

Isolation and Quantification of Cyclospora cayetanensis Oocysts from Cilantro

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We obtained C. cayetanensis oocysts from a known clinical sample C5 by a purification method described previously [11 (link), 17 (link)]. Briefly, C. cayetanensis oocysts were recovered from sieved fecal samples by differential sucrose and cesium chloride gradient centrifugations. Cyclospora cayetanensis oocysts were counted using a haemocytometer and fluorescent microscopy using a Zeiss Axio Imager D1 microscope (Zeiss, Oberkochen, Germany) with an HBO mercury short arc lamp and a UV filter (350 nm excitation and 450 nm emission). Then, known numbers of purified oocysts were added onto cilantro. DNA was extracted from this spiked cilantro sample as described in Murphy et al. [9 (link)]. Briefly, 25 g of cilantro sample were spiked with 200 oocysts, incubated at 4 °C for 48 h and washed with 0.1% Alconox (Alconox Inc., NY, USA). After centrifugation of the produce wash, debris pellets were collected, and DNA extracted using the FastDNA SPIN Kit for soil (MP Bio, Santa Ana, California).
Cinar H.N., Gopinath G., Murphy H.R., Almeria S., Durigan M., Choi D., Jang A., Kim E., Kim R., Choi S., Lee J., Shin Y., Lee J., Qvarnstrom Y., Benedict T.K., Bishop H.S, & da Silva A. (2020). Molecular typing of Cyclospora cayetanensis in produce and clinical samples using targeted enrichment of complete mitochondrial genomes and next-generation sequencing. Parasites & Vectors, 13, 122.
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8

Labeling and Imaging of BMPRII Variants

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COS7 or HEK293T cells grown on glass coverslips in six-well plates were transfected with 1 μg of DNA of myc-BMPRII-SF, myc-BMPRII-LF, or myc-BMPRII-LF-AA. After 24 h, cells were incubated (30 min, 37°C) in serum-free DMEM, washed with cold HBSS/HEPES/BSA (20 mM HEPES, pH 7.2, 2% BSA), blocked with normal goat γ-globulin (200 μg/ml, 30 min, 4°C), and labeled with anti-myc (20 μg/ml, 1 h, 4°C), followed by Alexa 546 GαM Fab′ (40 μg/ml, 30 min, 4°C), all in HBSS/HEPES/BSA. After washing at 4°C, cells were fixed with 4% paraformaldehyde in PBS (30 min, 22°C). Labeled slides were mounted with ProLong antifade reagent (Life Technologies). Fluorescence images were recorded with a CoolSNAP HQ-M camera (Photometrics, Tucson, AZ) using a 63×/1.4 numerical aperture oil immersion objective mounted on an AxioImager D.1 microscope (Carl Zeiss Microimaging, Jena, Germany).
Amsalem A.R., Marom B., Shapira K.E., Hirschhorn T., Preisler L., Paarmann P., Knaus P., Henis Y.I, & Ehrlich M. (2016). Differential regulation of translation and endocytosis of alternatively spliced forms of the type II bone morphogenetic protein (BMP) receptor. Molecular Biology of the Cell, 27(4), 716-730.
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9

GFP-POP-1 Asymmetry Observation in C. elegans

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All strains for this set of experiments were grown at 25°. We marked α and β cells in red (arIs208 and arIs222) to facilitate scoring GFP-POP-1 at a developmental stage based on the size of the gonad and on the size and position of the α and β cells. This stage was chosen to be analogous to the stage at which GFP-POP-1 begins to accumulate asymmetrically in the daughters of Z1 and Z4, as this asymmetry develops after their birth (Siegfried et al. 2004 (link)).
The four α and β cells are a similar small size when born. Soon after, the β cells become larger than the α cells, which remain small during the AC/VU decision. We examined L2 larvae in which the gonad size remained small (prior to elongation), but the difference in size between α and β cells was becoming apparent. We mounted worms of this stage and looked for visible differences in expression level between α and β cells. For qIs74; arIs208, we used a Zeiss Cell Observer Z1 SD with a Photometrics Evolve EMCCD camera, and for strains containing hlh-2prox::GFP and GFP-POP-1 transgenes, we used a Zeiss Axio Imager D1 microscope with a Zeiss AxioCam MRm camera.
Sallee M.D., Aydin T, & Greenwald I. (2015). Influences of LIN-12/Notch and POP-1/TCF on the Robustness of Ventral Uterine Cell Fate Specification in Caenorhabditis elegans Gonadogenesis. G3: Genes|Genomes|Genetics, 5(12), 2775-2782.
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10

Neutrophil Adhesion Assay in Lung Endothelial Cells

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The neutrophil adhesion assay was performed as described previously (104 (link)). Briefly, neutrophils were resuspended in RPMI 1640 medium (2 × 106 cells/ml) and labeled with 3 μM calcein AM (Life Technologies). Immediately before the labeled neutrophils were added, 48-well plates containing monolayers of HMVEC-lung cells were washed three times with RPMI 1640 medium containing 3% BSA. The labeled neutrophils were then added at 3 × 105 to 6 × 105 cells per well and allowed to incubate for 20 min at 37°C in the dark before being washed five times with PBS to remove nonadherent cells. Pre- and post-wash fluorescence were read at an excitation of 485 nm and an emission of 520 nm in a FLUOstar OPTIMA fluorescent plate reader. The relative adherence was then calculated. Neutrophil adhesion was visualized with a Zeiss Axio Imager D1 microscope.
Wilhelmsen K., Xu F., Farrar K., Tran A., Khakpour S., Sundar S., Prakash A., Wang J., Gray N.S, & Hellman J. (2015). Extracellular signal–regulated kinase 5 promotes acute cellular and systemic inflammation. Science signaling, 8(391), ra86.
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