Ab108821

Manufactured by Abcam

Sourced in United Kingdom, United States

Ab108821 is a lab equipment product. It serves a core function as a tool for use in laboratory settings.

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Lab products found in correlation

Ab100741, by Abcam (2 mentions) Mob00, by R&D Systems (2 mentions) Synergy ht, by Agilent Technologies (2 mentions) Synergy h1 hybrid multi mode reader, by Agilent Technologies (2 mentions) Ab205574, by Abcam (2 mentions) Ab100718, by Abcam (2 mentions) Ab108785, by Abcam (2 mentions) μquant, by Agilent Technologies (1 mentions) Hr series nefa kit, by Fujifilm (1 mentions) Synergy h1 hybrid reader, by Agilent Technologies (1 mentions) Ezrmi 13k, by Merck Group (1 mentions) Ezml 82k, by Merck Group (1 mentions) Ab136933, by Abcam (1 mentions) Mouse trap, by Immunodiagnostic Systems (1 mentions) Ac 33f1, by Immunodiagnostic Systems (1 mentions) Gen5 data analysis software, by Agilent Technologies (1 mentions) Powerwave ht, by Agilent Technologies (1 mentions) Advia 2400 chemistry system, by Siemens (1 mentions) Ezrl 83k, by Linco Research (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Corticosterone ELISA kit ab108821 (2 citations)

38 protocols using ab108821

Quantifying Brain IL-6 and Serum Corticosterone

Cited 2 times

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As we previously described,
¹³ (link),
¹⁴ (link) brain tissues were homogenized on ice in 20 mM Tris–HCL buffer containing protease inhibitors (10 mg/mL aprotinin, 5 mg/mL pepstatin, 5 mg/mL leupeptin and 1 mM phenylmethanesulfonylfluoride). The solutions were centrifuged at 13,000 rpm for 20 min at 4°C. The supernatant was saved and the BCA protein assay of the supernatant was performed for each sample. ELISA kit for measuring IL‐6 (M6000B, R&D Systems) was used to quantify the contents of this cytokine in the samples according to the manufacturer's instructions. The quantity of IL‐6 in each sample was standardized to the protein contents. The content of IL‐6 in the serum was measured in the same way. ELISA kit for measuring corticosterone (Ab108821, Abcam) was used to quantify the amount of corticosterone in the serum.

Jiang Q., Guo M, & Zuo Z. (2023). Familiar observers attenuate surgery‐induced neuroinflammation and cognitive dysfunction in mice. CNS Neuroscience & Therapeutics, 30(2), e14351.

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ELISA Assay of Plasma Samples

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Plasma samples were diluted 1:100 with assay buffer, and the enzyme-linked immunosorbent assay (ELISA) was performed according to the manufacturer’s instructions (catalog no. ab108821, Abcam, Cambridge, UK).

Marchette R.C., Carlson E.R., Said N., Koob G.F, & Vendruscolo L.F. (2022). Extended access to fentanyl vapor self-administration leads to addiction-like behaviors in mice: Blood chemokine/cytokine levels as potential biomarkers. Addiction neuroscience, 5, 100057.

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Corticosterone Quantification by ELISA

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Analysis of corticosterone was performed using an ELISA kit (ab108821; Abcam, Cambridge, MA) and a microplate spectrophotometer (μ‐Quant; Bio‐Tek Instruments, Winooski, VT) as previously described (Gulemetova and Kinkead 2011 (link)). Corticosterone concentrations were calculated from the parameters of the standard curve linearized by a log–log transformation.

Zhuang J., Zhao L, & Xu F. (2014). Maternal nicotinic exposure produces a depressed hypoxic ventilatory response and subsequent death in postnatal rats. Physiological Reports, 2(5), e12023.

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ELISA Assay of Plasma Samples

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Quantifying Serum Biomarkers

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Serum free fatty acids were quantified using an HR Series NEFA kit (Wako Diagnostics, Richmond, VA, USA). Serum catecholamines were quantified using an ELISA kit (E4462, Biovision, Milpitas CA, USA). Serum glucocorticoids were quantified using an ELISA kit (Ab108821, Abcam, Cambridge, MA, USA).

Cao Q., Wang S., Wang H., Cui X., Jing J., Yu L., Shi H, & Xue B. (2021). Fatty Acids Rescue the Thermogenic Function of Sympathetically Denervated Brown Fat. Biomolecules, 11(10), 1428.

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Serum Corticosterone Quantification by ELISA

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Serum corticosterone concentration was determined using an ELISA kit (ab108821; Abcam). Reagents and standards were prepared according to the manufacturer’s instructions. Cross reactivities for the kit were <30% deoxycorticosterone, <2% aldosterone, <2% progesterone, and <1% cortexolone. Recoveries were 96% with a sensitivity of 0.3 ng/ml. After diluting samples by 200x, the reaction was carried out in duplicate according to kit instructions. The average absorbance of each plate was determined using a plate reader and subtracting the absorbance at 450 nm from the absorbance at 570 nm (from manufacturer’s instructions) using a BioTek Synergy H1 Hybrid Reader. Corticosterone concentrations were extrapolated from a standard curve using a log-log transformation (better fit than four-parameter logistic curve fit in this instance), and then corrected for the dilution factor (1:200). Intra- and interassay variations were 5.5% and 8.3%, respectively.

Ashley N.T., Sams D.W., Brown A.C, & Dumaine J.E. (2016). Novel environment influences the effect of paradoxical sleep deprivation upon brain and peripheral cytokine gene expression. Neuroscience letters, 615, 55-59.

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Multiplex ELISA Measurements in Plasma

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For the leptin ELISA (MOB00, R&D Systems) measurements, blood plasma was 20x diluted and bone marrow plasma was 2x diluted. For the CXCL12 ELISA (ab100741, Abcam) measurements, bone marrow plasma was 4x diluted. For corticosterone ELISA (ab108821, Abcam) measurements, blood plasma was 50x diluted. Samples were analyzed according to the manufacturers’ instructions.

Frodermann V., Rohde D., Courties G., Severe N., Schloss M.J., Amatullah H., McAlpine C.S., Cremer S., Hoyer F.F., Ji F., van Koeverden I.D., Herisson F., Honold L., Masson G.S., Zhang S., Grune J., Iwamoto Y., Schmidt S.P., Wojtkiewicz G.R., Lee I.H., Gustafsson K., Pasterkamp G., de Jager S.C., Sadreyev R.I., MacFadyen J., Libby P., Ridker P., Scadden D.T., Naxerova K., Jeffrey K.L., Swirski F.K, & Nahrendorf M. (2019). Exercise reduces inflammatory cell production and cardiovascular inflammation via instruction of hematopoietic progenitor cells. Nature medicine, 25(11), 1761-1771.

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Corticosterone Measurement in Mice

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Blood was collected in the morning between 9:00 to 11:00 from mice tail vein. Before sampling, mice were placed in a warming cabinet (39°C for 10 to 15 min) in order to dilate the blood vessel. The levels of corticosterone were measured using an ELISA kit (ab108821, Abcam) following the manufacturer protocol.

Maiarù M., Tochiki K.K., Cox M.B., Annan L.V., Bell C.G., Feng X., Hausch F, & Géranton S.M. (2016). The stress regulator FKBP51 drives chronic pain by modulating spinal glucocorticoid signaling. Science translational medicine, 8(325), 325ra19.

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Circadian Rhythm of Serum Corticosterone

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Orbital sinus blood was collected under anesthesia at 8:00 AM and 8:00 PM on the day before the beginning of SCVS (before SCVS), and the day after the completion of SCVS (after SCVS). For each collection, ~100 μL whole blood was collected into 0.5 mL tube, sat for 1 h at room temperature for clotting, and centrifuged for 15 min at 956 × g. The supernatants were collected as serum and stored at −80 °C until analysis. Serum corticosterone concentrations were measured with an ELISA kit (Abcam, Ab108821), following the manufacturer’s instructions.

Dai W., Yang M., Xia P., Xiao C., Huang S., Zhang Z., Cheng X., Li W., Jin J., Zhang J., Wu B., Zhang Y., Wu P.H., Lin Y., Wu W., Zhao H., Zhang Y., Lin W.J, & Ye X. (2022). A functional role of meningeal lymphatics in sex difference of stress susceptibility in mice. Nature Communications, 13, 4825.

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Serum Insulin, Leptin, and Corticosterone Assay

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To determine the concentration of serum insulin, leptin and corticosterone we used commercial ELISA kits (#EZRMI-13K and #EZML-82K from MILLIPORE; Billerica, MA, USA and #ab108821 from ABCAM, Cambridge, UK). Enzymatic colorimetric method was used to measure serum glucose levels.

Campolim C.M., Weissmann L., Ferreira C.K., Zordão O.P., Dornellas A.P., de Castro G., Zanotto T.M., Boico V.F., Quaresma P.G., Lima R.P., Donato J J.r., Veras M.M., Saldiva P.H., Kim Y.B, & Prada P.O. (2020). Short-term exposure to air pollution (PM2.5) induces hypothalamic inflammation, and long-term leads to leptin resistance and obesity via Tlr4/Ikbke in mice. Scientific Reports, 10, 10160.

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