To enable fluorescence for CLSM, Endoflas F. S. sealer was combined with 0.1% Acridine orange fluorescent dye (maximum absorption: 570 nm, maximum emission: 720 nm). A K-file was used to coat the mixture of fluorescent dye and sealer in anticlockwise direction along the canal. Gutta-percha F3 size cones were selected as master cones, and the fit was verified by placing it to the working length. The authors performed lateral condensation technique for obturation along with the use of gutta-percha accessory cones. A heated instrument trimmed the excess cone and hand pluggers aided in vertical compaction. Radiographs ensured the absence of voids. The access cavity was sealed with Cavit-G (3M ESPE, USA).
Cavit g
Manufactured by 3M
Sourced in Germany, United States
Cavit G is a laboratory equipment product manufactured by 3M. It is designed for use in various scientific and analytical applications, providing a controlled environment for various processes and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Ah plus, by Dentsply (4 mentions)
Calamus 3d obturation system, by Dentsply (3 mentions)
Ah plus sealer, by Dentsply (2 mentions)
Calamus, by Dentsply (1 mentions)
No 10 k file, by Dentsply (1 mentions)
Largal ultra, by Septodont (1 mentions)
Endo z bur, by Dentsply (1 mentions)
Proroot mta, by Dentsply (1 mentions)
Ah plus root canal sealer, by Dentsply (1 mentions)
Filtek z250, by 3M (1 mentions)
μct50, by Scanco (1 mentions)
Gutta percha cones, by Dentsply (1 mentions)
Minocycline, by Ratiopharm (1 mentions)
Paper points, by Dentsply (1 mentions)
Reciproc r25, by VDW (1 mentions)
Silver reciproc, by VDW (1 mentions)
10 k file, by VDW (1 mentions)
24 protocols using cavit g
1
Fluorescent Root Canal Obturation
2
Endodontic Obturation Techniques Comparison
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The selected teeth specimens (n = 80) were then randomly assigned into 4 experimental group (n = 20 for each group) by using a simple random sampling method.
Group I: This group comprised of 20 prepared teeth and was left unfilled. (Negative Control)
Group II:This group comprised of 20 teeth and were obturated with flowable, dual cure composite resin (Para Core Dual Cure, ColteneWhaledent. Switzerland) (Positive Control)
Group III: This group comprised of 20 teeth and were obturated with thermoplasticised gutta-percha (Calamus, Dentsply Maillefer, Switzerland) and AH-plus sealer (Dentsply Maillefer, Switzerland) (Experimental Group)
Group IV: This group comprised of 20 teeth and were obturated with resilon and realseal sealer (Sybron Endo) (Experimental Group)
All procedures for the 4 groups were performed according to manufacturer's instructions. The root canal openings of all specimens were sealed with a non-eugenol temporary filling material, cavit G (ESPE, 3M, St. Paul, MN).
Subsequent to obturation, all root specimens were stored for 2 weeks in an incubator (Obromax) at 370 C in 100% humidity to allow the sealer to set completely. Each apical root end was then embedded in a polyvinyl ring, leaving 9mm of the root exposed and 5mm embedded. Acrylic resin was poured into polyvinyl ring, on a stable and smooth base. Each root was mounted when the acrylic resin was in dough stage.
Group I: This group comprised of 20 prepared teeth and was left unfilled. (Negative Control)
Group II:This group comprised of 20 teeth and were obturated with flowable, dual cure composite resin (Para Core Dual Cure, ColteneWhaledent. Switzerland) (Positive Control)
Group III: This group comprised of 20 teeth and were obturated with thermoplasticised gutta-percha (Calamus, Dentsply Maillefer, Switzerland) and AH-plus sealer (Dentsply Maillefer, Switzerland) (Experimental Group)
Group IV: This group comprised of 20 teeth and were obturated with resilon and realseal sealer (Sybron Endo) (Experimental Group)
All procedures for the 4 groups were performed according to manufacturer's instructions. The root canal openings of all specimens were sealed with a non-eugenol temporary filling material, cavit G (ESPE, 3M, St. Paul, MN).
Subsequent to obturation, all root specimens were stored for 2 weeks in an incubator (Obromax) at 370 C in 100% humidity to allow the sealer to set completely. Each apical root end was then embedded in a polyvinyl ring, leaving 9mm of the root exposed and 5mm embedded. Acrylic resin was poured into polyvinyl ring, on a stable and smooth base. Each root was mounted when the acrylic resin was in dough stage.
3
Gutta-Percha Obturation and AH Plus Sealing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The root canals were obturated with gutta-percha and the AH Plus (Dentsply DeTrey, Konstanz, Germany) root canal sealer using the Calamus 3D Obturation System
(Dentsply Maillefer, Ballaigues, Switzerland) and the warm vertical compaction technique. The root canal filling quality was checked with radiographs taken from the
mesio-distal and bucco-lingual directions. Samples with insufficient and/or inhomogeneous root canal fillings were replaced. Following restoration with a temporary
filling material (Cavit-G; 3M Espe, Seefeld, Germany), the samples were incubated in 37°C and fully saturated conditions for 14 days, in order to enable the complete
setting of the sealer in the root canal.
(Dentsply Maillefer, Ballaigues, Switzerland) and the warm vertical compaction technique. The root canal filling quality was checked with radiographs taken from the
mesio-distal and bucco-lingual directions. Samples with insufficient and/or inhomogeneous root canal fillings were replaced. Following restoration with a temporary
filling material (Cavit-G; 3M Espe, Seefeld, Germany), the samples were incubated in 37°C and fully saturated conditions for 14 days, in order to enable the complete
setting of the sealer in the root canal.
4
Standardized Root Canal Preparation and Obturation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The no. 10 k-file (Dentsply Maillefer, Ballaigues, Switzerland) was used to establish the canal patency. All the samples were prepared to the size A1 (size 40, 0.04 taper) Neoniti (Neolix, Châtres-la-Forêt, France) rotary file. The canals were irrigated during instrumentation procedure with 3% sodium hypochlorite (Novo Dental Products Pvt. Ltd, Mumbai, India) saline and 17% ethylenediaminetetraacetic acid (Largal Ultra, Septodont, Saint Maitre, France) throughout the shaping and cleaning procedure and dried with paper points (Kerr Corp., Romulus, MI) followed by obturation with the same size and taper gutta-percha with Cold lateral compaction. GuttaFlow was used as root canal sealer (Coltene/Whaledent, Langenau, Germany) in all the samples.
The teeth were temporized using Cavit G (3M ESPE, Germany) and stored at 37°C in 100% humidity for 1 week to allow complete sealing of the sealer. Teeth were radiographed in buccolingual and mesiodistal directions to assess the radiographic adequacy of root filling, using the following criteria: reaching the working length, uniform radiopacity, and no voids.
The teeth were temporized using Cavit G (3M ESPE, Germany) and stored at 37°C in 100% humidity for 1 week to allow complete sealing of the sealer. Teeth were radiographed in buccolingual and mesiodistal directions to assess the radiographic adequacy of root filling, using the following criteria: reaching the working length, uniform radiopacity, and no voids.
5
Calcium Hydroxide and Laser Treatment for Root Canal
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In group 4, the same protocol was performed as in group 2 and followed by laser irradiation as in group 3.
All the root canals were dried with paper points, and calcium hydroxide (Ca(OH)2) paste (Calsin; Karabağlar, Izmir, Turkey) was applied as intra-canal medicament. A piece of sterile cotton was placed in the pulp chamber, and a temporary restorative material (Cavit-G; 3 M ESPE, St Paul, MN) was used to seal the access cavity. The patients received training on how to complete the numerical rating scale sheets at home (Additional file2 ), and they were instructed to record their PP scores on these sheets after 8, 12, 24 and 48 h and after 7 days. The patients were advised that they could take 600 mg of ibuprofen every 8 h for pain alleviation if they suffered from severe pain at any point and that they should record the time interval to medication intake.
At the second visit, the Ca(OH)2 was removed from the root canals with final irrigation and using a 25/0.06 file in mesial canals and 35/0.06 in distal canals. The root canals were dried after Ca(OH)2 paste removal and obturated using the modified single cone technique. Subsequently, the teeth were restored using a temporary restorative material, and the patients were referred for permanent restorations.
All the root canals were dried with paper points, and calcium hydroxide (Ca(OH)2) paste (Calsin; Karabağlar, Izmir, Turkey) was applied as intra-canal medicament. A piece of sterile cotton was placed in the pulp chamber, and a temporary restorative material (Cavit-G; 3 M ESPE, St Paul, MN) was used to seal the access cavity. The patients received training on how to complete the numerical rating scale sheets at home (Additional file
At the second visit, the Ca(OH)2 was removed from the root canals with final irrigation and using a 25/0.06 file in mesial canals and 35/0.06 in distal canals. The root canals were dried after Ca(OH)2 paste removal and obturated using the modified single cone technique. Subsequently, the teeth were restored using a temporary restorative material, and the patients were referred for permanent restorations.
6
Minimally Instrumented Root Canal with Antibiotic Paste
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Local anesthesia (LA) was administered using 2% lignocaine without vasoconstrictor by infiltration technique. Under rubber dam isolation and aseptic conditions, the access cavity was prepared using sterile No:1 Endo access and Endo ‘Z’ bur (Dentsply Maillefer, USA). After working length (WL) determination, minimal instrumentation was done and the canal was irrigated using 10 ml of 1% sodium hypochlorite (NaOCl) (Chenchems Reagent, Chenchemicals, Chennai, India) for 2 min with side vented needles placed 1 mm short of WL. The canals were dried using paper points 2 mm short of the WL. 0.02 mg each of ciprofloxacin, metronidazole and amoxicillin was mixed in the ratio of 1:1:1 with 0.5 mL of propylene glycol to obtain a final concentration of 0.12 mg/mL which was placed in the coronal third of the root canal. The pulp chamber was then temporarily restored with Cavit G (3 M ESPE, Germany) and a radiograph was taken. The patient was recalled after 3 weeks.
7
Removal of Intracanal Medicaments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the canals were apically sealed using sticky wax to ensure containment of the ICMs inside the root canal throughout the required duration. All the ICMs used, whether in the form of a paste or a gel, were injected inside the root canals. The coronal openings were sealed with a small cotton pellet and temporary filling (Cavit G, 3M ESPE, Seefeld, Germany). The specimens were stored at 37°C in 100% humidity for 3 weeks [17 (link)] after which the ICMs were removed by a combination of light instrumentation using the master apical file followed by rinsing with 10 mL of 2.5% NaOCl, 10 mL of 17% EDTA for 1 minute, and then 10 mL of distilled water.
8
Dental Materials Composition and Properties
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The materials used in the study were Pro Root MTA (DENTSPLY Tulsa Dental Specialties, Johnson City, TN), Light Cure Universal Restorative Glass Ionomer cement (GC Corporation, Tokyo, Japan), GC Dentin conditioner — 10% polyacrylic acid (GC Corporation, Tokyo, Japan), Cavit G (3M ESPE, Germany), cold cure acrylic (Acralyn R, India) composition as shown in Table 1 .
9
Disinfection Protocol for Root Canals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Irrigation was done with 5 ml of normal saline with hypodermic syringe and 27-gauge, beveled needle, which was kept 1 mm short of the working length. Sterile water was used to rinse and the canals were dried with paper points. An immediate postoperative sample was taken as described for Group A (Sample D2).
After obtaining the second sample for each group, the canals were dried and Cavit™ G (3M ESPE, Germany) was used for coronal seal. The patients were recalled after 7 days. In the second appointment, root canal was reopened under rubber dam isolation and was subjected to one more application of disinfection protocol as per groups. The third sample was taken as described before (Sample A3, B3, C3, and D3).
After obtaining the second sample for each group, the canals were dried and Cavit™ G (3M ESPE, Germany) was used for coronal seal. The patients were recalled after 7 days. In the second appointment, root canal was reopened under rubber dam isolation and was subjected to one more application of disinfection protocol as per groups. The third sample was taken as described before (Sample A3, B3, C3, and D3).
10
Bioceramic Obturation Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The EndoSequence verifier that fit the prepared canal space was selected and then the corresponding C points are used for obturation. Premixed bioceramic sealer is placed into the canal with the provided syringe tip up to 2/3rd of the canal. Matched taper cone (C points) was dipped into the sealer and placed slowly in up and down motion until it reaches the full working length. The cone is then seared off at the orifice level.
In all the samples obturating material is removed 3 mm beneath the cementum-enamel junction and restored with Cavit G (3M ESPE, Seefeld, Germany) and stored at more than 95% relative humidity at 37°C for 10 days in a humidifier. All the samples were vertically sectioned using a hard tissue microtome as the chances of crack formation in the tooth structure as well as the material will be minimal. Marginal gap at sealer and root dentin interface was evaluated under scanning electron microscope at ×2000 magnification at coronal, and apical halves of root canal.
In all the samples obturating material is removed 3 mm beneath the cementum-enamel junction and restored with Cavit G (3M ESPE, Seefeld, Germany) and stored at more than 95% relative humidity at 37°C for 10 days in a humidifier. All the samples were vertically sectioned using a hard tissue microtome as the chances of crack formation in the tooth structure as well as the material will be minimal. Marginal gap at sealer and root dentin interface was evaluated under scanning electron microscope at ×2000 magnification at coronal, and apical halves of root canal.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!