The deoxyHb was prepared by bubbling nitrogen into an HbA (oxy) solution (60 µM) containing 0.01% poly-t-dimethylsiloxane (0.01% anti-foaming reagent) at room temperature for 20 minutes. DeoxyHb formation and purity were confirmed by spectral scanning using an Agilent 8453 spectrophotometer. The deoxygenation process was regarded as complete when the shoulder peak at 576 nm is completely diminished [26 ].
8453 spectrophotometer
Manufactured by Agilent Technologies
Sourced in United States, Germany
The 8453 spectrophotometer is a high-performance UV-Visible spectrophotometer designed for a wide range of applications. It features a diode array detector and a wavelength range of 190 to 1100 nm. The instrument provides accurate and reliable measurements of absorbance, transmittance, and concentration.
Automatically generated - may contain errors
Lab products found in correlation
Cary 5000 spectrophotometer, by Agilent Technologies (3 mentions)
C18 macrospin column, by Nest Group (3 mentions)
Vector 22 spectrometer, by Bruker (2 mentions)
Avance 400, by Bruker (2 mentions)
Avance 300, by Bruker (2 mentions)
Avance 3 600 mhz spectrometer, by Bruker (2 mentions)
Jem 1010 microscope, by JEOL (2 mentions)
Jsm 6700f feg, by JEOL (2 mentions)
Dpx 400 spectrometer, by Bruker (2 mentions)
Rf 540 fluorescence spectrophotometer, by Agilent Technologies (2 mentions)
2400 series 2 chns o analyzer, by PerkinElmer (2 mentions)
Ez melt mpa120, by Stanford Research Systems (2 mentions)
400 mhz ft nmr, by Bruker (2 mentions)
Micromass zq mass spectrometer, by Waters Corporation (2 mentions)
Frontier ft ir, by PerkinElmer (2 mentions)
1100 series hplc system, by Agilent Technologies (2 mentions)
Tensor 27 ft ir instrument, by Bruker (2 mentions)
Maldisynapt instrument, by Waters Corporation (2 mentions)
P4 2080 plus hplc system, by Agilent Technologies (2 mentions)
P 1010 polarimeter, by Jasco (2 mentions)
109 protocols using 8453 spectrophotometer
1
Deoxygenation of Hemoglobin Solution
2
Synthesis and Characterization of Bis(hexafluoroacetylacetonato)copper-(II)
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bis(hexafluoroacetylacetonato)copper-(II) [abbreviated as Cu(hfac)2] was prepared and purified by previously described procedures [48 ]. Other chemicals were of the highest purity commercially available and were used as received. The progress of reactions was monitored by thin-layer chromatography (TLC) on Silica gel 60 F254 aluminum sheets with hexane or CHCl3 as the eluent. Column chromatography was carried out on silica gel (0.063–0.200 mm). NMR spectra were recorded for 2a ,b solutions in CDCl3 on Bruker Avance-300 (300.13 MHz for 1H, 282.25 MHz for 19F) and Avance-400 (400.13 MHz for 1H, 100.62 MHz for 13C) spectrometers; chemical shifts (δ) of 1H and 13C{1H} are given in ppm, with the solvent signals serving as the internal standard (δH = 7.24 ppm, δC = 76.9 ppm); the internal standard for 19F spectra was C6F6 (δ = −162.9 ppm). Fourier transform infrared (FT-IR) spectra were acquired in KBr pellets on a Bruker Vector-22 spectrometer. UV-vis spectra were registered on an HP Agilent 8453 spectrophotometer (in 10−5–10−4 M solutions in EtOH). Masses of molecular ions were determined by high-resolution mass spectrometry (HRMS) by means of a DFS Thermo Scientific instrument (EI, 70 eV). Melting points were recorded on a Melter-Toledo FP81 Thermosystem apparatus. Elemental analyses were performed using a Euro EA 3000 elemental analyzer.
3
Comprehensive Material Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
UV–vis–NIR absorption
spectra were recorded using an Agilent 8453 spectrophotometer. Transmission
electron microscopy was performed using a JEOL JEM 1010 microscope
operating at an acceleration voltage of 100 kV. EDX analysis was performed
using a JEOL 2100F field emission electron microscope equipped with
an energy-dispersive X-ray (EDX) spectrometer, operating at an accelerating
voltage of 200 kV. AFM images were collected on dry samples using
a Multimode 8 Nanoscope V (Veeco) in the tapping mode and an NCHV-A
cantilever (antimony (n)-doped Si, tip ROC < 10 nm, K = 40 N m–1, frequency 339–388 KHz). In
the case of hydrated samples, AFM images on were recorded using the
Peak Force QNM mode and a ScanAsyst-Fluid cantilever (silicon nitride,
tip ROC < 10 nm, K = 0.7 N m–1, frequency 150 KHz).
spectra were recorded using an Agilent 8453 spectrophotometer. Transmission
electron microscopy was performed using a JEOL JEM 1010 microscope
operating at an acceleration voltage of 100 kV. EDX analysis was performed
using a JEOL 2100F field emission electron microscope equipped with
an energy-dispersive X-ray (EDX) spectrometer, operating at an accelerating
voltage of 200 kV. AFM images were collected on dry samples using
a Multimode 8 Nanoscope V (Veeco) in the tapping mode and an NCHV-A
cantilever (antimony (n)-doped Si, tip ROC < 10 nm, K = 40 N m–1, frequency 339–388 KHz). In
the case of hydrated samples, AFM images on were recorded using the
Peak Force QNM mode and a ScanAsyst-Fluid cantilever (silicon nitride,
tip ROC < 10 nm, K = 0.7 N m–1, frequency 150 KHz).
4
Spectroscopic Characterization of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1H NMR and 13C NMR spectra were recorded using Bruker ARX-400 (Bruker, Billerica, MA, USA) at 400 MHz for 1H and 100.6 MHz for 13C, Bruker Biospin Avance III 400 at 400 MHz for 1H and 100.6 MHz for 13C, Bruker Ascend™-400 at 400 MHz for 1H and 100.6 MHz for 13C or in a Bruker AVANCE™-600 AT 600 MHz for 1H and 150 MHz for 13C spectrometers. Mass spectra were performed in a mass spectrometer with electronic impact ionization (70 eV) Thermo Scientific DSQ II Single Quadrupole GC/MS with Focus GC. The HRMS (Electrospray Ionization Time of Flight, ESI-TOF) mass spectra (MS) were performed on a High Resolution Mass Spectrometer Orbitrap, Q-Exactive (Thermo Fisher Scientific, Waltham, MA, USA) Electrospray Ionization (H-ESI-II), coupled to a Liquid Chromatographer uHPLC Ultimate 2000 Dionex (Thermo Fisher Scientific, Waltham, MA, USA). Matrix-Assisted Laser Desorption/Ionization (MALDI) experiments were performed on a 4700 Proteomics Analyzer MALDI-TOF mass spectrometer. UV–Vis absorption spectra were registered on a Hewlett-Packard 8452A Diode Array spectrophotometer and an AGILENT 8453 spectrophotometer (Santa Clara, CA, USA).
5
Characterization of Dendrimer Conjugates
6
Spectroscopic Analysis of Solutions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The pH values of solutions were determined using a Metrohm 780 pH-meter (Metrohm AG, Herisau, Switzerland). An Agilent (Agilent Technologies, Inc., Santa Clara, CA, USA) 8453 spectrophotometer with diode array detection was utilized to record the spectra of solutions from 350 to 650 nm at 1 nm intervals. A graphical user interface for MCR-ALS calculations was used which is available for free. MCR-ALS calculations were performed in MATLAB 7.5 (MathWorks, Natick, MA, USA). Minitab 16.2.4 (Minitab Inc., State College, PS, USA) software was used to design experiments, obtain the model and plot response surfaces.
7
Sedimentation Velocity Analysis of ChuX
8
Optical and Structural Characterization of Materials
9
Optical and Structural Characterization of Materials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical characterization was carried out by UV-vis-NIR spectroscopy with a Cary 5000 spectrophotometer and Agilent 8453 spectrophotometer. Transmission electron microscopy (TEM) analysis was performed by using a JEOL JEM 1010 microscope operating at an acceleration voltage of 100 kV. Samples for TEM in the case of Au@TiO2 substrates were obtained by scratching the films from the substrate and depositing them on carbon- and FORMVAR-coated copper grids. The surface morphology of the dried gels was investigated by using a scanning electron microscope (JEOL JSM-6700F FEG).
Raman Spectroscopy experiments were conducted with a Renishaw InVia Reflex system. The spectrograph used a high-resolution grating (1200 or 1800 grooves mm−1) with additional band-pass filter optics, a confocal microscope, and a 2D-CCD camera. Several laser excitation energies were employed, including laser lines at 633 (HeNe) and 785 and 830 nm (diode).
Scanning electron microscopy analysis was performed with a JEOL JSM-6700F or with a Helios NanoLab 450S Scanning Electron Microscopes.
Raman Spectroscopy experiments were conducted with a Renishaw InVia Reflex system. The spectrograph used a high-resolution grating (1200 or 1800 grooves mm−1) with additional band-pass filter optics, a confocal microscope, and a 2D-CCD camera. Several laser excitation energies were employed, including laser lines at 633 (HeNe) and 785 and 830 nm (diode).
Scanning electron microscopy analysis was performed with a JEOL JSM-6700F or with a Helios NanoLab 450S Scanning Electron Microscopes.
10
Synthesis and Characterization of Mn-Oxo Complexes
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!