Anti lc3b 1 2

RPMI-1640 and fetal bovine serum (FBS) were bought from HIMEDIA (Mumbai, India) and HyClone (Thermo Scientific Hy-Clone, Logan, Utah). Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was purchased from Sisco Research Laboratory (Mumbai, India). 3-MA was purchased from Sigma Aldrich (USA). Anti-Beclin-1, anti-LC3B-I/II, anti-Caspase-3 and HRP-tagged secondary antibodies were purchased from Abcam (Cambridge, UK) respectively. Other chemicals used in this study were of highest experimental grade available.

Pentobarbital sodium was purchased from Amresco (Cleveland, OH, USA). Krebs-Henseleit Bicarbonate Buffer was purchased from M&C GENE Technology (Beijing) Ltd. (Beijing, China). anti-IL-6 monoclonal antibody (anti-IL-6 mAb) for rats was purchased from InvivoGen (CA, USA). JAK1/2 inhibitor ruxolitinib (S1378) was purchased from Selleck Chemicals (Houston, TX). The primary antibodies including the anti-IL-6, anti-p-JAK1/2, anti-JAK1/2, anti-p-STAT3/5, anti-STAT3/5, and anti-LC3B-I/II were purchased form Abcam (Shanghai, China). MitoSOX™ Red Mitochondrial Superoxide Indicator was purchased from Invitrogen (CA, USA). Myosin Heavy Chain (NOQ7.5.4D) (ab11083), anti-Fast Myosin Skeletal Heavy Chain (MY-32) (ab51263), and anti-laminin were purchased from Abcam (Shanghai, China). Hydrogen Peroxide Assay Kit was purchased from BioVision (Palo Alto, CA, USA). Enzyme-linked immunosorbent assay (ELISA) kits for TNF-α (Cat no. RTA00), IL-1β (Cat no. RLB00), and IL-6 (Cat no. R6000B) were purchased from R&D Systems Inc. (Minneapolis, MN, USA). BCA protein assay kit was purchased from Beyotime (Shanghai, China). Lactate Assay Kit was purchased from BioVision (CA, USA).