Primary antibodies used were anti-TBK1 (Alexis Biochemicals, Lausen, Switzerland), anti-TRIF (Abnova, Walnut, CA), anti–TNFR-associated factor 3 (TRAF3) (Santa Cruz Biotechnoly), and anti-β-actin (Abcam, Cambridge, U.K.), anti-IRF3 (Santa Cruz Biotechnologies), anti-Flag (Sigma-Aldrich), anti-Xpress (Invitrogen Life), Anti-α-Actinin (H-300) (Santa Cruz Biotechnologies), anti-ICP0 (Santa Cruz Biotechnologies), anti-IRF5 (Cell Signalling), anti-IRF7 (Abcam).
Anti icp0
Manufactured by Santa Cruz Biotechnology
Sourced in United Kingdom, United States
Anti-ICP0 is a laboratory reagent used in research applications. It is an antibody that specifically detects the ICP0 protein, which is an immediate-early regulatory protein expressed by the herpes simplex virus. The core function of Anti-ICP0 is to serve as a tool for the identification and study of the ICP0 protein in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh, by Santa Cruz Biotechnology (3 mentions)
Anti gfp, by Abcam (2 mentions)
Reducing sample buffer, by Bio-Rad (2 mentions)
Protease inhibitor cocktail, by Merck Group (2 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Santa Cruz Biotechnology (2 mentions)
Precast polyacrylamide gel, by Bio-Rad (2 mentions)
Anti mr1 ct, by Abcam (2 mentions)
Anti gd, by Santa Cruz Biotechnology (2 mentions)
Endo h, by New England Biolabs (2 mentions)
Anti β actin, by Merck Group (2 mentions)
Mouse anti gfp, by Merck Group (2 mentions)
Anti α tubulin, by Santa Cruz Biotechnology (2 mentions)
Anti histone h3, by Abcam (2 mentions)
Rabbit anti gfp, by Abcam (2 mentions)
Anti ha, by Abcam (2 mentions)
Anti ifi16, by Santa Cruz Biotechnology (2 mentions)
Anti slfn5, by Merck Group (2 mentions)
Anti slfn11, by Novus Biologicals (2 mentions)
Anti icp8, by Santa Cruz Biotechnology (2 mentions)
Anti tk, by Santa Cruz Biotechnology (2 mentions)
8 protocols using anti icp0
1
Primary Antibodies for Immune Signaling
2
Antibody Detection Protocol for Herpes Virus
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Anti-GAPDH, anti-ICP0, and anti-UL42 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, United States). Monoclonal antibody against Us11 was kindly provided by Professor Bernard Roizman. Anti-rabbit and anti-mouse IgG HRP (horseradish peroxidase) secondary antibodies were also from Santa Cruz Biotechnology.
3
MR1 and HLA-A,B,C Protein Detection
4
Monitoring HSV-1 Infection in Vero Cells
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HSV1(F) (provided by B. Roizman, University of Chicago, USA) was used for infection experiments. The strain HSV1(17+)lox (provided by B. Sodeik, Hannover Medical School, Germany) was used as PCR template. HSV1 propagation and virus growth curves were performed as described [14 (link)]. To monitor infection, Vero cells were infected with HSV1(F) at the indicated MOI. Cell lysates were prepared at the indicated times post infection and analysed by Western blotting using primary antibodies to the immediate early proteins ICP0 (anti-ICP0, Santa Cruz) and ICP27 (anti-ICP27, Virusys), to the early protein gB (anti-Glykoprotein B, Santa Cruz) and to the late proteins VP5 (anti-ICP5 (VP5), Abcam) and pUL34 [9 (link)] followed by secondary antibodies conjugated to POX. Antibodies specific to β-actin (Abcam) were used as control.
5
Comprehensive Antibody Characterization Protocol
6
Antibody Characterization for SLFN5, SLFN11, and DNA Repair
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The following antibodies were used: anti-SLFN5 (Sigma-Aldrich; Cat.HPA017760; Lot.B96361), anti-SLFN11 (Novus Biologicals; Cat.NBP1-92368; Lot.H96783), anti-PML (Bethyl Laboratory; Cat.A301-167A, Santa Cruz; Cat.sc-966; Lot.H1413), anti-IFI16 (Santa Cruz; Cat.sc-8023; Lot.C1312), anti-ATRX (Santa Cruz; Cat.sc-15408), anti-DNA-PKcs (Santa Cruz; Cat.sc-5282; Lot.G280), anti-SUMO2+3 (Abcam; Cat.ab3742; Lot.GR8249-1), anti-RNAP II (Santa Cruz; Cat.sc-56767), anti-GFP rabbit (Abcam; Cat.ab290; Lot.GR3251545-1), anti-GFP mouse (Millipore; Cat.MAB2510; Lot.2512480), anti-RAD50 (GeneTex; Cat.GTX70228; Lot.40186), anti-V5 (Santa Cruz; Cat.sc-271944; Lot.E2217), anti-HA (Abcam; Cat.ab9110; Lot.GR3217183-2), anti-GAPDH (GeneTex; Cat.GTX100118; Lot.42158), anti-α-Tubulin (Santa Cruz; Cat.sc-69969; Lot.DO412), anti-β-Actin (Sigma-Aldrich; Cat.a5441; Lot.064M4789V), anti-KU70 (Abcam; Cat.ab83501; Lot.GR3176811-2), anti-Histone H3 (Abcam; Cat.ab1791; Lot.GR3198176-1), anti-ICP0 (Santa Cruz; Cat.sc-53070; Lot. A0313), anti-ICP8 (gifted from David M. Knipe), anti-TK (Santa Cruz; Cat.sc-28037; Lot.K1813), anti-VP21 and anti-gD (gifted from Gary H. Cohen), anti-IE1/IE2 (Virusys; Cat.P1215; Lot.A1345070), anti-UL44 (Virusys; Cat.ca006-100; Lot.C1034151), adenovirus late protein antibody staining Hexon, Penton and Fiber (gift from James M. Wilson), and anti-DBP (gift from Arnold J. Levine).
7
MR1 and HLA-A,B,C Protein Detection
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Cells were harvested in cell lysis buffer (50 mM NaCl, 50 mM TRIS pH8, 1% IGEPAL, 1% Triton X-100) supplemented with protease inhibitor cocktail (Sigma) and allowed to incubate on ice for 20 mins. Lysates were then centrifuged (16,000 × g for 20 min at 4°C) and the supernatant collected. Lysates were mock or Endo H (NEB) digested according to the manufacturer’s instructions for 90 mins at 37°C as required. Lysates were denatured by heating at 95°C for 5 mins in reducing sample buffer (Bio-Rad) and resolved by SDS-PAGE on precast polyacrylamide gels (Biorad) before immunoblotting onto PVDF membranes. Membranes were probed with the designated primary antibodies in 3% BSA in PBST, followed by incubation with an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (all Santa Cruz Biotechnology). The following primary antibodies were utilized: anti-MR1 CT (McWilliam et al., 2016 (link)), anti-MR1 and anti-HLA-A, B, C (Abcam), anti-GFP, anti-GAPDH, anti-gD, and anti-ICP0 (all Santa Cruz Biotechnology).
8
SARS-CoV-2 and Herpes Virus Immunofluorescence
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Cells were pelleted at 16,000 xg for 1 min and resuspended in 50 μL PBS. Cells were added to a μ-Slide 18 well (Ibidi, # 81826) for 2 s. Slides were allowed to dry for 30 min at room temperature before inactivation with 100% acetone for 30 min at room temperature. SARS2-CoV-2 samples were blocked with 1% BSA in PBS, stained with anti-N hybridoma 5D4 supernatant diluted 1:10 in 1% BSA in PBS, and stained with anti-mouse AF488 diluted 1:500 and DAPI at 1 ug/mL. The SARS2-CoV-2 antibody was a kind gift from Petra Emmerich. PBS with 1% FBS 0.1% Triton X-100 was used to block HSV-1 and HCMV samples and dilute the antibodies. Anti-ICP0 (Santa Cruz Biotechnology, # sc-53070) diluted 1:100 was used for HSV-1, and anti-IE1/2 hybridoma 3H4 supernatant diluted 1:3 was used for HCMV.
Images were acquired with a Nikon Eclipse Ti2 body equipped with a Yokogawa CSU-W1 spinning disk, Andor iXon Ultra DU-888U3 EMCCD camera, and Plan Apo 20x objective. Pixel resolution was 655 nm/pixel. Illumination was performed with a 405 nm and 488 nm laser through a quad filter (405/488/568/647), and emission light was acquired with a 447/60 and 525/50 filter for DAPI and AF488, respectively.
Images were acquired with a Nikon Eclipse Ti2 body equipped with a Yokogawa CSU-W1 spinning disk, Andor iXon Ultra DU-888U3 EMCCD camera, and Plan Apo 20x objective. Pixel resolution was 655 nm/pixel. Illumination was performed with a 405 nm and 488 nm laser through a quad filter (405/488/568/647), and emission light was acquired with a 447/60 and 525/50 filter for DAPI and AF488, respectively.
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