D5000 nikkor lens af s micro nikkor 60 mm

The photographs of gross plant morphology at 10 days after seed sowing (DAS) and 12 DAS were taken with a stereoscopic microscope (M165FC; Leica Microsystems) connected to a CCD camera (DFC300FX; Leica Microsystems) and a digital camera (D5000 Nikkor lens AF-S Micro Nikkor 60 mm; Nikon). The gross plant morphology at 25 DAS was photographed with a digital camera (D5000 Nikkor lens AF-S Micro Nikkor 60 mm; Nikon). After sampling, the leaves were fixed in formalin/acetic acid/alcohol [FAA; 4% (v/v) formalin, 5% (v/v) acetic acid, and 50% (v/v) ethanol] and cleared with chloral solution (200-g chloral hydrate, 20-g glycerol, and 50-ml deionized water) to measure the leaf area and cell number, as described previously (Tsuge et al., 1996 (link)). The whole leaves were observed with a stereomicroscope equipped with a CCD camera. The leaf palisade tissue cells were observed and photographed under a light microscope (DM-2500; Leica Microsystems) equipped with Nomarski differential interference contrast optics and a CCD camera. The cell size was determined as the mean palisade cell area of 20 palisade cells per leaf on a paravermal view (Ferjani et al., 2011 (link)).

Photographs of the gross plant phenotypes at 10 days after sowing (DAS) were obtained using a stereoscopic microscope (M165FC; Leica Microsystems) connected to a charge-coupled device (CCD) camera (DFC300FX; Leica Microsystems) and those at 21 DAS were obtained using a digital camera (D5000 Nikkor lens AF-S Micro Nikkor 60 mm; Nikon). Leaves were fixed in formalin/acetic acid/alcohol and cleared with chloral solution (200 g of chloral hydrate, 20 g of glycerol, and 50 mL of deionized water) to measure leaf area and cell number, as described previously (Tsuge et al., 1996 (link)). Whole leaves were observed using a stereoscopic microscope equipped with a CCD camera. Leaf palisade tissue cells were observed and photographed under a light microscope (DM-2500; Leica Microsystems) equipped with Nomarski differential interference contrast optics and a CCD camera. Cell size was determined as mean palisade cell area, determined from a paradermal view, as described previously (Ferjani et al., 2011 (link)).

Photographs of the gross plant phenotypes at 10 DAS were taken with a stereoscopic microscope (M165FC; Leica Microsystems) connected to a CCD camera (DFC300FX; Leica Microsystems), and those at 25 DAS were taken with a digital camera (D5000 Nikkor lens AF-S Micro Nikkor 60 mm; Nikon).
Cotyledons were fixed in formalin/acetic acid/alcohol and cleared with chloral solution (200 g chloral hydrate, 20 g glycerol, and 50 mL deionized water) to measure cotyledon areas and cell numbers, as described previously [72 (link)]. Whole cotyledons were observed using a stereoscopic microscope equipped with a CCD camera. Cotyledon palisade tissue cells were observed and photographed under a light microscope (DM-2500; Leica Microsystems) equipped with Nomarski differential interference contrast optics and a CCD camera. Cell size was determined as the mean palisade cell area, detected from a paravermal view, as described previously [23 (link)]. The cotyledon aspect ratio was calculated as the ratio of the cotyledon blade length to width.