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The E7F7N is a high-quality laboratory equipment product from Cell Signaling Technology. It is designed to perform a core function within the research and development workflow, but a detailed description cannot be provided while maintaining an unbiased and factual approach. Additional information about the intended use or specific applications of this product is not available.

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Lab products found in correlation

Af2997, by R&D Systems (1 mentions) A3854, by Merck Group (1 mentions) Cay10006421, by Cayman Chemical (1 mentions)

2 protocols using e7f7n

1

Western Blot Analysis of HIF-1α and HIF-2α

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Cells were lysed in UREA/SDS buffer, and proteins were resolved by SDS-PAGE. After transferring the proteins onto polyvinylidene difluoride membranes, proteins were detected using a rabbit polyclonal anti-HIF-1α antibody (Cay10006421, rabbit polyclonal, Cayman Chemicals) or a goat polyclonal HIF-2α antibody (AF2997, R&D Systems). We further used a rabbit monoclonal anti-DPF3 antibody (E7F7N, #82788, Cell Signaling), a mouse monoclonal anti-beta actin antibody (A3854, Sigma Aldrich), and horseradish peroxidase-conjugated secondary antibodies as applicable (Dako, Agilent Technologies).
Protze J., Naas S., Krüger R., Stöhr C., Kraus A., Grampp S., Wiesener M., Schiffer M., Hartmann A., Wullich B, & Schödel J. (2022). The renal cancer risk allele at 14q24.2 activates a novel hypoxia-inducible transcription factor-binding enhancer of DPF3 expression. The Journal of Biological Chemistry, 298(3), 101699.
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2

Immunostaining Analysis of HIF-1α, HIF-2α, and DPF3

Cited 2 times
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Immunostainings for HIF-1α and HIF-2α were conducted on paraffin-embedded tissue arrays as described earlier (11 (link)). For DPF3, we used a rabbit monoclonal antibody against DPF3 (E7F7N, #82788, Cell Signaling) at a dilution of 1:5000 and an antigen retrieval procedure (Dako, Agilent Technologies). Stainings were analyzed independently by two researchers and scored based on intensity of nuclear staining (0–4) and percent of positive cells (0–100%) using an IRS according to Remmele and Stegner (32 (link)). For comparison of the IRS, HIF and DPF3 stainings were divided into four categories: no (IRS = 0), low (IRS 1–3), intermediate (IRS 4–8), or high (9 (link), 10 , 11 (link), 12 (link)) levels of immunoreactivity.
Protze J., Naas S., Krüger R., Stöhr C., Kraus A., Grampp S., Wiesener M., Schiffer M., Hartmann A., Wullich B, & Schödel J. (2022). The renal cancer risk allele at 14q24.2 activates a novel hypoxia-inducible transcription factor-binding enhancer of DPF3 expression. The Journal of Biological Chemistry, 298(3), 101699.
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