High affinity anti ha rat and anti ha affinity matrix

The rabbit phospho-site–specific GPR35 antiserum pSer³⁰⁰/pSer³⁰³-hGPR35a (Cat number (7TM0102C), raised against the sequence KAHKpSQDpSLCVTL, and the pSer²⁹⁸/pSer³⁰¹-mGPR35 antiserum (7TM0102B), raised against the sequence TPHKpSQDpSQILSLT, were developed in collaboration with 7TM Antibodies GmbH. IRDye 800CW donkey anti-rabbit IgG, IRDye 800CW donkey anti-goat IgG, and IRDye 800CW goat anti-rat IgG were from LI-COR Biosciences. Alexa Fluor 488-goat anti-rabbit IgG and Alexa Fluor 594-donkey anti-rat IgG were from Abcam. Mouse monoclonal anti-FLAG M2 was from Merck. Horseradish peroxidase anti-mouse (sheep) was from GE Healthcare. High affinity anti-HA (rat) and anti-HA affinity matrix were from Roche Diagnostics.

A rabbit phospho-site–specific hGPR35 antiserum pSer³⁰⁰/pSer³⁰³-hGPR35a (catalogue number 7TM0102C), raised against the sequence KAHKpSQDpSLCVTL, and a pSer²⁹⁸/pSer³⁰¹-mGPR35 antiserum (7TM0102B), raised against the sequence TPHKpSQDpSQILSLT have been described previously (5 ). A GPR35 (nonphospho) antibody (cat number 7TM0102N), directed against the distal part of the carboxyl-terminal tail of hGPR35 was developed in collaboration with 7TM Antibodies GmbH. IRDye 800CW donkey anti-rabbit immunoglobulin G (IgG), IRDye 800CW donkey anti-goat IgG, and IRDye 800CW goat anti-rat IgG were from LI-COR Biosciences. Horseradish peroxidase anti-mouse (sheep) was from GE Healthcare. High-affinity anti-HA (rat) and anti-HA affinity matrix were from Roche Diagnostics. Anti-LgBiT mAb (cat number N7100) which is an affinity-purified mouse mAb for detection of LgBiT and LgBiT-fusion proteins by Western blotting was purchased from Promega Corporation. GRK isoform–directed antisera for GRK 2 sc-13143 (C-9) c and GRK 5 sc-518005 (D-9) were from Santa Cruz Biotechnology while GRK 3 CS #80362 (D8G6V) and GRK 6 CS #5878 (D1A4) were from Cell Signaling Technology. Further details are found in Reichel et al. (23 (link)).