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Pe cy7 conjugated annexin 5

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PE-Cy7-conjugated annexin V is a fluorescent protein used in flow cytometry applications. It binds to phosphatidylserine, which is exposed on the cell surface during apoptosis. This allows for the detection and quantification of apoptotic cells.

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Lab products found in correlation

Pe conjugated cd34, by BD (1 mentions) 7 aad, by BD (1 mentions) Hoechst 33342, by Solarbio (1 mentions)

2 protocols using pe cy7 conjugated annexin 5

1

Flow Cytometry Analysis of Hematopoietic Cells

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Antibodies used for flow cytometry analysis were as follows: mouse monoclonal antibody against human band 3 was generated in our laboratory and labelled with FITC or APC as described previously,26, 27 PE‐conjugated CD34 (BD #555822), APC‐conjugated CD71 (BD #551374), PE‐conjugated CD235a/GPA (eBioscience #5555570) and APC‐conjugated α4 integrin (BD #130‐093‐281). Other reagents are PE‐Cy7‐conjugated annexin V (eBioscience #88‐8103‐74), Hoechst33342 (Solarbio #C0031) and 7AAD (BD #559925).
Wang S., Zhao H., Zhang H., Gao C., Guo X., Chen L., Lobo C., Yazdanbakhsh K., Zhang S, & An X. (2022). Analyses of erythropoiesis from embryonic stem cell‐CD34+ and cord blood‐CD34+ cells reveal mechanisms for defective expansion and enucleation of embryomic stem cell‐erythroid cells. Journal of Cellular and Molecular Medicine, 26(8), 2404-2416.
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2

Apoptosis Detection by Flow Cytometry

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After induction of apoptosis, thymocytes were washed once in PBS and suspended in Annexin binding buffer. Thereafter, the cells were stained with PE.Cy7-conjugated Annexin V (eBioscience) and DAPI as per the manufacturer’s instructions. After staining, cells were washed with Annexin binding buffer and suspended with PBS. Apoptotic cells were evaluated by flow cytometry and considered as AnnexinV+/DAPI- cells.
Dolitzky A., Hazut I., Avlas S., Grisaru-Tal S., Itan M., Zaffran I., Levi-Schaffer F., Gerlic M, & Munitz A. (2022). Differential regulation of Type 1 and Type 2 mouse eosinophil activation by apoptotic cells. Frontiers in Immunology, 13, 1041660.
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