The expression of calprotectin and tryptase was studied by using immunohistochemistry. The paraffin-embedded histologic tissue sections of the stomach, jejunum, ileum, and colon segments were deparaffinized. Endogenous peroxidase activity was blocked by incubating the tissue sections in 3% hydrogen peroxide for 10 minutes. The tissue section was then incubated with primary antibodies, anti-calprotectin (1:200; Abcam, Cambridge, MA, USA) and anti-mast cell tryptase (1:500; Abcam), followed by the secondary antibody anti-mouse IgG (1:1000; Santa Cruz Biotechnology, Dallas, TX, USA). Then, the sections were incubated with strep-tavidin-horseradish peroxide for 30 minutes followed by treatment with AB-peroxidase solution and counterstaining with hematoxylin. Positively stained cells were then examined under a fluorescence microscope (Zeiss Axio Imager Z1; Carl Zeiss, Jena, Germany).
Anti calprotectin
Manufactured by Abcam
Sourced in United States, Macao
Anti-calprotectin is a laboratory equipment product used to detect and measure the presence of calprotectin, a protein involved in inflammatory processes. It can be used for research purposes to study the role of calprotectin in various biological and medical contexts.
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Lab products found in correlation
2 protocols using anti calprotectin
1
Immunohistochemical Analysis of Calprotectin and Tryptase
2
Kidney Biopsy Analysis Protocol
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Immunofluorescence: biopsy samples of kidney cortex were processed for cryosectioning. Antibodies: Anti Factor B (Novus Biologicals, UK), anti C4d, anti C1q (AbD Serotec, France), anti C3c, anti C5b-9 (DAKO, France), anti MBL, anti MASP2 (Cliniscience, France). Each slide was semi quantitatively evaluated by a trained nephropathologist.
Immunohistochemistry: biopsies were preserved in formalin. Antibodies: anti-vimentin (Cell-Marque, USA), anti-αSMA (alpha smooth muscle actin); (Sigma, St. Louis, MO), anti-Calprotectin reacting with Monocyte/Granulocytes/Macrophages (MAC-387, AbCam, France) and anti-CD3 (SouthernBiotech, Birmingham, Alabama, USA). Fibrosis was analyzed by Sirius Red staining. Staining quantification was performed in silico (Visilog 6.9 software): fibrosis: percent of stained area per field; inflammation: number of positive immune cells per field. We evaluated 10 fields (×100) per tissue sample.
Immunohistochemistry: biopsies were preserved in formalin. Antibodies: anti-vimentin (Cell-Marque, USA), anti-αSMA (alpha smooth muscle actin); (Sigma, St. Louis, MO), anti-Calprotectin reacting with Monocyte/Granulocytes/Macrophages (MAC-387, AbCam, France) and anti-CD3 (SouthernBiotech, Birmingham, Alabama, USA). Fibrosis was analyzed by Sirius Red staining. Staining quantification was performed in silico (Visilog 6.9 software): fibrosis: percent of stained area per field; inflammation: number of positive immune cells per field. We evaluated 10 fields (×100) per tissue sample.
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