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Enzychrom free acid assay kit

Manufactured by BioAssay Systems
Sourced in United States

The EnzyChrom™ Free Acid Assay Kit is a colorimetric assay kit designed to quantify free acid concentrations in samples. The kit provides reagents and a protocol to measure the optical density of the sample, which is proportional to the free acid content.

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3 protocols using enzychrom free acid assay kit

1

Metabolic Profiling of Euthanized Mice

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After the mice were euthanized, blood samples were collected and centrifuged for serum separation. The concentration of glucose was measured by a glucometer (Bayer Contour, Tarrytown, NY, USA). The concentration of insulin was determined by a Mouse Ultrasensitive insulin ELISA Kit (ALPCO Diagnostics, Salem, NH, USA). The content of free fatty acids was measured using an EnzyChrom™ Free Acid Assay Kit (BioAssay System, CA, USA).
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2

Metabolic Profiling of Euthanized Mice

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After the mice were euthanized, blood samples were collected and centrifuged for serum separation. The concentration of glucose was measured by a glucometer (Bayer Contour, Tarrytown, NY, USA). The concentration of insulin was determined by a Mouse Ultrasensitive insulin ELISA Kit (ALPCO Diagnostics, Salem, NH, USA). The content of free fatty acids was measured using an EnzyChrom™ Free Acid Assay Kit (BioAssay System, CA, USA).
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3

Serum Biomarker Quantification

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The content of serum free fatty acids was determined using an EnzyChrom Free Acid Assay Kit (BioAssay System, CA, USA). Serum triglyceride and total cholesterol levels were measured using colourimetric assay kits (Thermo-Scientific, Ann Arbor, MI, USA). Serum insulin concentration was measured using a Mouse Ultrasensitive Insulin ELISA Kit (ALPCO Diagnostics, Salem, NH, USA).
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