In vivo growth, nude mice were injected with a total of 6 × 106 cells suspended in 200 μL of a mixture of serum-free RPMI 1640/Matrigel (1:1 volume) (BD Biosciences, MA) into the left axillary fossa. For metastasis assay, 2 × 106 cells suspended in 200 μL of serum-free RPMI 1640 were injected into nude mice via tail vein injection. Eight weeks later, the mice were sacrificed, and the tumors and lung tissues were excised and fixed with 4% phosphate-buffered neutral formalin for at least 72 h. Metastatic tissues were analyzed by H&E staining. All of the experiments were approved by the Shanghai Medical Experimental Animal Care Commission.
Rpmi 1640 matrigel
Manufactured by BD
Sourced in United States
RPMI 1640/Matrigel is a cell culture medium and extracellular matrix product. RPMI 1640 is a widely used cell culture medium that provides nutrients for the growth and maintenance of various cell types. Matrigel is a gelatinous protein mixture derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells, which can be used as an extracellular matrix to support cell attachment, migration, and differentiation.
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Lab products found in correlation
4 protocols using rpmi 1640 matrigel
1
In vivo Tumor Growth and Metastasis
2
Orthotopic Liver Tumor Implantation in Mice
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Mice were placed in the supine position. A 1- to 1.5-cm skin incision was made in the upper abdominal wall, followed by a 1-cm incision in the peritoneum to expose the liver. The left lobe of the liver was moved outside the body and placed on a nonwoven absorbent fabric sheet as previously described [18 (link)]. 1.0 × 106 UM001-tdTomato cells in 20 μl of 2:1 RPMI 1640/Matrigel (BD Biosciences, Bedford, MA, USA) were gently injected under the surface of the left lobe of the liver (Fig. 1 e). The insertion site of the needle was cauterized and sealed with absorbable hemostatic material. The liver was returned within the body after the injection, and the abdominal incision was closed in 2 layers with 5-0 polydioxanone absorbable thread (AD Surgical, Sunnyvale, CA, USA).
3
Evaluating Tumor Growth in Xenograft Models
4
Xenograft and Metastasis Assay
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Invivo growth, nude mice were injected with a total of 6× 10 6 cells suspended in 200μl of a mixture of serum-free RPMI 1640/Matrigel (1:1 volume) (BD Biosciences, MA) into the left axillary fossa. For metastasis assay, 2× 10 6 cellssuspended in 200μl ofserum-free RPMI 1640 injected into nude mice via tail vein injection. Eight weeks later, the mice were sacri ced, and the tumors and lung tissues were excised and xed with 4% phosphate-buffered neutral formalin for at least 72 h. Metastatic tissues were analyzed by H&E staining. All of the experiments were approved by the Shanghai Medical Experimental Animal Care Commission.
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