Anhydrous ethanol

Manufactured by Chron Chemicals

Sourced in China

Anhydrous ethanol is a laboratory-grade solvent and reagent. It is a clear, colorless liquid with a characteristic odor. Anhydrous ethanol has a high purity level and contains minimal water content, making it suitable for various scientific and analytical applications requiring a dry, pure alcohol medium.

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Lab products found in correlation

14 protocols using anhydrous ethanol

Synthesis of Silica-based Materials

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Tetraethylorthosilicate (TEOS) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Methyltriethoxysilane (MTES), Hexamethyldisiloxane (HMDS), decane, and sec-butanol was purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China). Anhydrous ethanol, heptane, and ammonia water were purchased from Chengdu Kelong Chemical Co., Ltd. (Chengdu, China). The water was deionized. The TEOS and MTES were distillation purified. Other chemicals were used without further purification.

Yang W., Lei X., Hui H., Zhang Q, & Deng X. (2018). Fabrication of Refractive Index Tunable Coating with Moisture-Resistant Function for High-Power Laser Systems Based on Homogeneous Embedding of Surface-Modified Nanoparticles. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(5), 1105.

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Chitosan Modification via TEMPO-Mediated Oxidation

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Commercial CS was obtained from Sigma-Aldrich (Beijing, China). The viscosity-average molecular weight (Mv) of 1000 kDa and the deacetylation degree (DD) of 88% were determined by the Mark–Houwink–Sakurada equation and ¹H NMR spectroscopy [24 (link)]. Succinic anhydride (C₄H₄O₃, assay >99%, SA) was furnished by Beijing InnoChem Science & Technology Co., Ltd. (Beijing, China). TEMPO (assay >98%) was purchased from Acros Organics (Shanghai, China). Sodium hypochlorite pentahydrate (NaClO·5H₂O) was obtained from TCI Shanghai (Shanghai, China). Acetone (CH₃COCH₃) was of analytical grade and furnished by Changlian Chemical Reagent Industries, Ltd. (Chengdu, China). Chemicals dimethyl sulfoxide (DMSO), sodium hydroxide (NaOH), sodium bromide (NaBr), hydrochloric acid (HCl), and anhydrous ethanol (CH₃CH₂OH) were of analytical grade and purchased from Chengdu Kelong Chemical Co., Ltd. (Chengdu, China). Chitosan was ground by a Baijie BJ-200 stainless steel grinder to pass through a 100-mesh sieve and used for further study. Other reagents were used without further purification.

Li A., Xue Q., Ye Y., Gong P., Deng M, & Jiang B. (2020). Study on TEMPO-Mediated Oxidation of N-Succinyl Chitosan and the Water Retention Property. Molecules, 25(20), 4698.

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Synthesis and Characterization of Cadmium-based Materials

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Cadmium acetate (Cd(CH₃COO)₂·2H₂O, AR), sodium sulfide (Na₂S, AR), and thioacetamide (CH₃CSNH₂, AR) were obtained from Aladdin Co. Ltd (Shanghai, China). Thiourea (NH₂CSNH₂, AR) and thiosemicarbazide (NH₂CSNHNH₂, AR) were purchased from Adamas Reagent, Ltd (Shanghai, China). Triethylenetetramine (C₆H₁₈N₄, AR) was provided by Xiya Reagent Co. Ltd (Shangdong, China). Anhydrous ethanol (C₂H₅OH, AR), rhodamine B (C₂₈H₃₁ClN₂O₃, AR) and lactic acid (C₃H₆O₃, AR) were purchased from Kelong Chemical Reagent Co. Ltd (Chengdu, China). All chemicals were used directly without further purification. Ultra-pure water was used for all experiments. All the data were measured in triplicate.

Zhu Q., Wang J., Chen S., Fu H., Zhang J., Gao H, & Liao Y. (2022). Effect of the organic sulfur source on the photocatalytic activity of CdS. RSC Advances, 12(18), 11262-11271.

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Synthesis and Purification of Silica Precursors

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Tetraethylorthosilicate (TEOS) was purchased from Sinopharm Chemical Reagent Co. Ltd. (Beijing, China). Hexamethyldisiloxane (HMDS) was purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China). Anhydrous ethanol and ammonia water were purchased from Chengdu Kelong Chemical Co., Ltd. (Chengdu, China). The water was deionized. The TEOS were distillation purified before use. Other chemicals were used without further purification.

You T.H., Yang W., Hui H.H., Lei X.Y., Wang T.Y., Zhang Q.H., Ju X, & Deng X.R. (2023). Damage analysis and mechanism study of sol–gel coating over KDP crystal under multi-pulse of laser irradiation at low flux. Scientific Reports, 13, 3451.

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Epoxy Resin Synthesis with Flame Retardants

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Diglycidyl ether of bisphenol A (DGEBA, E-51: epoxide value 0.51 mol/100 g) was acquired from Nantong Xingchen Synthetic Material Co., Ltd. (Nantong, China). Anhydrous ethanol and toluene were obtained from Chengdu Kelong Chemical Co., Ltd. (Chengdu, China). Vanillin (VN) was purchased from Shanghai Yuan Ye Biotechnology Co., Ltd. (Shanghai, China). 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide (DOPO) and 4,4-diaminodiphenylmethane (DDM) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). 2,4-Diamino-6-phenyl-1,3,5-triazine (DPT) was purchased from Wuhan Lanbai Pharmaceutical Chemical Co., Ltd. (Wuhan, China).

Yu Y., Chen J., Ding A., Wang C., Wang Y, & Yang L. (2024). Synthesis of a Novel P/N-Triazine-Containing Ring Flame Retardant and Its Application in Epoxy Resin. Polymers, 16(7), 871.

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Osteogenic Differentiation of MC3T3-E1 Cells

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Calcium chloride (CaCl₂), sodium dihydrogen phosphate (NaH₂PO₄), sodium hydroxide (NaOH), oleic acid, anhydrous ethanol and phosphate buffer (PBS) were obtained from Chengdu Kelong Chemical Co., Ltd. (Chengdu, Sichuan, China). Gelatin, methacrylic anhydride, ammonium persulfate (APS) and tetramethylethylenediamine (TEMED) were purchased from Aladdin Reagent Company (Shanghai, China). Fetal bovine serum (FBS) and α-Minimum Essential Medium (α-MEM) were purchased from Gibco (Grand Island, NY, USA). Streptomycin, penicillin and trypsin were purchased from Biosharp (Anhui, China). Dexamethasone, ascorbic acid-2 phosphate and β-glycerophosphate were purchased from Beijing Solarbio Sciences Co. (Beijing, China). Mouse calvaria-derived (subclone 14) osteoblast-like cells (MC3T3-E1) were purchased from Cobioer Co. (Nanjing, Jiangsu, China). The Sprague-Dawley rats used in this study were purchased from Chongqing Medical University Animal Care and Use Committee. All reagents were analytically pure and used as received. The experimental water was deionized water.

Wang H., Hu B., Li H., Feng G., Pan S., Chen Z., Li B, & Song J. (2022). Biomimetic Mineralized Hydroxyapatite Nanofiber-Incorporated Methacrylated Gelatin Hydrogel with Improved Mechanical and Osteoinductive Performances for Bone Regeneration. International Journal of Nanomedicine, 17, 1511-1529.

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Synthesis and Characterization of Iron-based Nanomaterials

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Sinopharm Chemical Reagent Co., Ltd provided FeCl₃·6H₂O (ferric chloride hexahydrate) and NaOH (sodium hydroxide) for use 15 tetracycline hydrochloride (TC) was purchased from Beijing Soleibao Technology Co., Ltd. Polyvinylpyrrolidone (PVPK30), potassium ferrocyanide trihydrat (K₄[Fe(CN)₆]·3H₂O), anhydrous ethanol (C₂H₆O), urea (CO(NH₂)₂), peroxide hydrogen (H₂O₂ 30%), ferric nitrate nonahydrate, hydrazine hydrate (Fe(NO₃)₃·9H₂O), sodium acetate anhydrous (N₂H₄·H₂O), and isopropanol (C₂H₃NaO₃) purchased from Chengdu Kelong Chemical Co., Ltd. Disodium EDTA was purchased from Guangdong Guanghua Technology Co., Ltd. l-Ascorbic acid, and sodium carboxymethyl cellulose (CMC) were purchased from Shanghai McLean Biochemical Co., Ltd. Sodium azide (NaN₃) and tetracycline hydrochloride (TC 96%) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. All chemicals and substances. The analytical level that can be obtained from commercial sources is used in the study.

Wei J., Yan C., Chen Y., Cheng Z., Qiu F., Tang C., Yang C., Wei Z, & Du A. (2024). Investigation of α-Fe2O3 catalyst structure for efficient photocatalytic fenton oxidation removal of antibiotics: preparation, performance, and mechanism. RSC Advances, 14(24), 16649-16660.

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Graphite-based Epoxy Composite Synthesis

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Natural
flake graphite
powder (purity 99.9%) with an average particle size of 500 mesh was
supplied by Qingdao Xingyuan Graphite Co., Ltd. (Shandong, China).
Concentrated sulfuric acid (98%), sodium nitrate, potassium permanganate,
hydrogen peroxide (30%), hydrochloric acid, anhydrous ethanol, acetic
acid, and acetone all were of analytical reagent grade and purchased
from Chengdu Kelong Chemical Co., Ltd. (Chengdu, China). DOPO was
supplied by Guangdong Wengjiang Chemical Reagent Co., Ltd. (Guangdong,
China). Vinyltriethoxysilane (VTES) was kindly supplied by Qufu Chenguang
Chemical Co., Ltd. (Shandong, China). Epoxy resin (E-51, epoxy value:
0.48–0.54 mol/100 g) was purchased from Hangzhou Wuhuigang
Adhesive Co., Ltd. (Hangzhou, China). 4,4-Diamino-diphenyl methane
(DDM) was obtained from Aladdin Industrial Corporation (Shanghai,
China).

Zhi M., Liu Q., Chen H., Chen X., Feng S, & He Y. (2019). Thermal Stability and Flame Retardancy Properties of Epoxy Resin Modified with Functionalized Graphene Oxide Containing Phosphorus and Silicon Elements. ACS Omega, 4(6), 10975-10984.

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Tetracycline Quantification in Milk

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The tetracycline (TC), oxytetracycline (OTC), chlorotetracycline (CTC), tetraethoxysilane (TEOS), ethylenediamine and 3-aminopropyl triethoxysilane (APTES) were purchased from Adamas-Beta® (Shanghai, China). Ammonia, diethyl ether, Na₂HPO₄, methanol, acetonitrile, acetone, anhydrous ethanol, Na₂EDTA and citric acid monohydrate were obtained from Chengdu Kelong Chemical Reagent Factory (Chengdu, China). The milk powder samples were purchased from markets in Chengdu (China). All chemicals were of analytical pure grade otherwise stated. Distilled water was used throughout. The stock standard solution of TC (1.0 mg mL⁻¹) was prepared by dissolving TC in distilled water. The working standard solutions were prepared by diluting stock standard solutions with distilled water. The Na₂EDTA-McIlvaine buffer (0.1 mol L⁻¹, pH 4.0) solution was produced by dissolving Na₂HPO₄ (8.86 g), Na₂EDTA (16.81 g) and citric acid monohydrate (5.90 g) in 500 mL of water.^{23 (link)}

Wang Q., Wu Y., Bao X., Yang M., Liu J., Sun K., Li Z, & Deng G. (2022). Novel fluorescence sensor for the selective recognition of tetracycline based on molecularly imprinted polymer-capped N-doped carbon dots. RSC Advances, 12(38), 24778-24785.

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Chromatographic and Spectrophotometric Analysis of Bioactive Compounds

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The instruments used were as follows: SHIMADZU Nexera X2 UPLC (Kyoto, Shimadzu, Japan), Applied Biosystems 4500 QTRAP mass spectrometer (Applied Biosystems, Waltham, MA, USA), MULTISKAN GO full-wavelength microplate reader (Thermo Fisher, Waltham, MA, USA), 3K15 centrifuge (Sigma-Aldrich, St. Louis, MO, USA), WP-UP-YJ-20 micro-organic heat removal ultra-pure water machine (Sichuan Water Treatment Equipment Co., Chengdu, China), and ME204E electronic balance (Shanghai Mettler-Toledo Instruments, Shanghai, China). The reagents used were as follows: 2,2-biphenyl-1-bitter hydrazinyl (Shanghai Macklin Biochemical Technology Co., Ltd., Shanghai, China), 2,2′-Hydrazinyl-bis(3-ethylbenzothiazoline-6-sulphonic acid) diamine salt (Shanghai Macklin Biochemical Technology Co., Ltd., Shanghai, China), glucose (Sigma-Aldrich, St. Louis, MO, USA), anhydrous ethanol (analytical grade, Chengdu Kelong Chemical Reagent Factory, Chengdu, China), phenol (analytical grade, Chengdu Kelong Chemical Reagent Factory, Chengdu, China), concentrated sulphuric acid (analytical grade, Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), acetonitrile (mass spectrometry grade, Merck & Co., Rahway, NJ, USA), and methanol (mass spectrometry grade, Merck & Co., Rahway, NJ, USA).

Yang D., Song Y., Lu A., Qin L., Tan D., Zhang Q., He Y, & Lu Y. (2023). Metabolomics-Based Analysis of the Effects of Different Cultivation Strategies on Metabolites of Dendrobium officinale Kimura et Migo. Metabolites, 13(3), 389.

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