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Hepes salt

Manufactured by Merck Group
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Sourced in Italy, United States

Hepes salt is a chemical compound commonly used as a buffering agent in laboratory applications. It maintains a stable pH environment within aqueous solutions, ensuring optimal conditions for various biological and chemical experiments.

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Lab products found in correlation

Sephadex g 75, by Merck Group (5 mentions) Pyrene, by Merck Group (4 mentions) Tween 20, by Merck Group (3 mentions) Cholesterol, by Merck Group (3 mentions) 1 6 diphenyl 1 3 5 hexatriene dph, by Merck Group (3 mentions) Human serum, by Merck Group (2 mentions) Calcein, by Merck Group (2 mentions) Mucin from porcine stomach type 2 powder, by Merck Group (2 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions) Span 20, by Merck Group (2 mentions) Tween 20 tw20, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Almond oil, by Merck Group (1 mentions) Ethanol, by Merck Group (1 mentions) Trypsin, by Merck Group (1 mentions) Pepsin, by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Formalin, by Merck Group (1 mentions) Trypan blue, by Merck Group (1 mentions)

Spelling variants of this product

HEPES (2817 citations) HEPES sodium salt (34 citations) HEPES potassium salt (3 citations) HEPES sodium salt pH 7.4 (3 citations) 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) sodium salt (2 citations) N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES) potassium salt (2 citations) 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium salt (HEPES) (2 citations)

14 protocols using hepes salt

1

Membrane Protein Interaction Characterization

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Tween 20, Almond Oil, Cholesterol, Hepes salt (N-(2-hydroxyethyl) piperazine-N-(2-ethanesulphonic acid)), pyrene, ethanol, trypsin, pepsin, sodium chloride and human serum were purchased by Sigma-Aldrich (St. Louis, MO, USA). BM635 and BM859 were prepared following a procedure reported in the Figures S1 and S2 from the Supplementary Materials.
Hanieh P.N., Consalvi S., Forte J., Cabiddu G., De Logu A., Poce G., Rinaldi F., Biava M., Carafa M, & Marianecci C. (2022). Nano-Based Drug Delivery Systems of Potent MmpL3 Inhibitors for Tuberculosis Treatment. Pharmaceutics, 14(3), 610.
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2

Lipid Composition Analysis Protocol

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Tween 20, cholesterol, CHEMS, Sephadex G75, HEPES salt (N-(2-idroxyethyl) piperazine-N-(2-ethanesulfonic acid)), zymosan A, formalin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) salt, and Trypan blue were purchased from Sigma-Aldrich SRL (Milan, Italy). Fetal bovine serum, 0.1% trypsin/0.02% ethylenediamine tetraacetic acid, phosphate-buffered saline, and Dulbecco’s Modified Eagle’s Medium were purchased from Gibco® Cell Culture Basics (Life Technology Italia, Monza, Italy). Diphenylhexatriene was obtained from Acros Organics BVBA (Geel, Belgium). The AG was a kind gift from Bernett (Pavia, Italy). The cellulose membrane dialysis tubing was purchased from Spectrum Laboratories (Prodotti Gianni SpA, Milan, Italy). Cellulose filters (0.45 μm) were obtained from Millipore (Billerica, MA, USA). Tissue culture test plates were purchased from TPP Tissue Cell Culture Products (Delchimica Scientific Glassware, Naples, Italy). Male CD-1 mice were purchased from Harlan Laboratories (Correzzana, Italy). All other chemicals used during the experiments were of analytical grade and no additional purification was carried out before analysis. Double-distilled water was used throughout the study.
Marianecci C., Rinaldi F., Di Marzio L., Mastriota M., Pieretti S., Celia C., Paolino D., Iannone M., Fresta M, & Carafa M. (2014). Ammonium glycyrrhizinate-loaded niosomes as a potential nanotherapeutic system for anti-inflammatory activity in murine models. International Journal of Nanomedicine, 9, 635-651.
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3

Functionalized Tween-20 Nanocarrier Synthesis

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Tween-20 (Tw20), Sephadex G-75, Hepes salt [N-(2-hydroxyethyl) piperazine-N-(2-ethanesulfonic acid)], calcein, Ibuprofen and Lidocaine were purchased from Sigma-Aldrich (Sigma-Aldrich SRL, Milan, Italy). Cholesterol (CHOL) and diphenylhexatriene (DPH) were obtained from Acros Organics (Acros Organics BVBA, Geel, Belgium). All other products and reagents were of analytical grade. Tween-20 derivative was synthesized as previously described: briefly, terminal hydroxyl groups were functionalized with glycine (GLY) following the Eschweiler–Clarke reaction13 (link),15 .
Rinaldi F., Del Favero E., Rondelli V., Pieretti S., Bogni A., Ponti J., Rossi F., Di Marzio L., Paolino D., Marianecci C, & Carafa M. (2017). pH-sensitive niosomes: Effects on cytotoxicity and on inflammation and pain in murine models. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 538-546.
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4

Characterization of Stilbene Compounds

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Tween 20 (Tw20), 3,5,4’-trihydroxy-trans-stilbene (RV), trans-1,2-diphenylethylene (TS), human serum (HS), Hepes salt (N-(2-hydroxyethyl) piperazine-N-(2-ethanesulphonic acid)), pyrene, Nile Red and Roswell Park Memorial Institute (RPMI) 1640 medium were Sigma-Aldrich products (Sigma-Aldrich, Milan, Italy). Neem oil was purchased from Neem Italia (Moniga del Garda (BS), Italy) and characterized by an ECOCERT certificate (Biocert Italia IT013BC041—ICEA 264BC001). All other products and reagents were of analytical grade.
Rinaldi F., Maurizi L., Forte J., Marazzato M., Hanieh P.N., Conte A.L., Ammendolia M.G., Marianecci C., Carafa M, & Longhi C. (2021). Resveratrol-Loaded Nanoemulsions: In Vitro Activity on Human T24 Bladder Cancer Cells. Nanomaterials, 11(6), 1569.
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5

Liposomal Delivery of Pentamidine

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HEPES salt (Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate), cholesterol, dicetyl phosphate (DCP), Sephadex G75, pentamidine isethionate, pyrene, 1,6-diphenyl-1,3,5-hexatriene (DPH), mucin from porcine stomach type II powder, Tween 20 (polysorbate 20), chitosan medium molecular weight powder, and sodium hydroxide were purchased from Sigma-Aldrich (Milan, Italy). l-Glutamic acid was supplied by PanReac Applichem (Milan, Italy). All other products and reagents were of analytical grade.
Rinaldi F., Hanieh P.N., Chan L.K., Angeloni L., Passeri D., Rossi M., Wang J.T., Imbriano A., Carafa M, & Marianecci C. (2018). Chitosan Glutamate-Coated Niosomes: A Proposal for Nose-to-Brain Delivery. Pharmaceutics, 10(2), 38.
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6

Formulation and Characterization of HSPC-DPPG Liposomes

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Hydrogenated phosphatidylcholine from soybean (HSPC) with molecular weight Mw = 790 g/mol and anionic 1,2-Dipalmitoyl-sn-glycero-3-phosphorylglycerol sodium salt (DPPG) with molecular weight Mw = 745 g/mol were a kind gift from LIPOID GmbH (Ludwigshafen, Germany). The typical fatty acid composition (expressed in % of total fatty acids) of HSPC is: Palmitic acid: (5.0–20.0%) and Stearic acid (80.0–95.0%). Sodium calcein, rifampicin (RIF) (nominal purity >97%), Sephadex G75, diphenylhexatriene (DPH), calcein, and Hepes salt [N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)] were purchased by Sigma-Aldrich (St. Louis, MO, USA). This drug has a LogP = 3719; thus, it is associated to a very low water solubility [H2O: soluble 2.5 mg/mL at 25 °C (pH 7.3) chloroform: soluble 50 mg/mL [17 (link)].
All other products and reagents were of analytical grade.
Rinaldi F., Hanieh P.N., Sennato S., De Santis F., Forte J., Fraziano M., Casciardi S., Marianecci C., Bordi F, & Carafa M. (2021). Rifampicin–Liposomes for Mycobacterium abscessus Infection Treatment: Intracellular Uptake and Antibacterial Activity Evaluation. Pharmaceutics, 13(7), 1070.
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7

Nanoparticle Formulation for Tumor Targeting

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DOX was supplied by Lancrix Chemicals (Shanghai, China), with a purity higher than 99.9%. Hydrogenated soy phosphatidylcholine (HSPC), egg phosphatidylglycerol (EPG), and Distearoyl-phosphoethanolamine-polyethilenglycol2000 (DSPE-PEG2000) were acquired from Lipoid GmbH (Ludwigshafen, Germany). Cholesterol (CHOL) and HEPES salt were supplied by Sigma Chemical Company (St. Louis, MO, USA). The polycarbonate membrane was purchased from Millipore (Billerica, MA, USA). All other compounds and solvents used in this work were of analytical grade. RPMI 1640 Medium, fetal bovine serum (FBS), penicillin, streptomycin, and trypsin EDTA 0.25% were purchased from Gibco-Invitrogen (Waltham, MA, USA). The subcutaneous tumor model was established in 7–8-week female BALB/c mice purchased from CEBIO-UFMG (Belo Horizonte, Brazil).
Maciel e Silva A.T., Maia A.L., Silva J.D., Miranda S.E., Cantini T.S., de Barros A.L., Soares D.C., de Magalhães M.T., Alves R.J, & Ramaldes G.A. (2023). In Vitro and Preclinical Antitumor Evaluation of Doxorubicin Liposomes Coated with a Cholesterol-Based Trimeric β-D-Glucopyranosyltriazole. Pharmaceutics, 15(12), 2751.
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8

Liposomal Formulation Development

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Tween 20 (polysorbate 20 or Polyoxyethylenesorbitan monolaurate), Span 20 (sorbitan monolaurate), cholesterol (Chol), Hepes salt (Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate), Sephadex G-75, Pyrene, 1,6-diphenyl-1,3,5-hexatriene (DPH), calcein, bovine serum, 4-bromobutyric acid, N-methyl 2-pyrrolidone (NMP), N-(3-dimethylaminopropyl)-N’-(ethylcarbodimide hydrochloride) (EDC∙HCl), and 4-(Dimethylamino)pyridine (DMAP) were purchased from Sigma-Aldrich. Hyaluronan sodium salt (HANa, Mw = 2.2 × 105) was provided by Contipro (Dolní Dobrouč, Czech Republic) and it was modified in hyaluronan tetrabutylammonium salt (HATBA) form using a Dowex cation exchange resin. 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was obtained from Avanti Polar Lipids (Alabaster, AL, USA). All other products and reagents were of analytical grade.
Hanieh P.N., Forte J., Di Meo C., Ammendolia M.G., Del Favero E., Cantù L., Rinaldi F., Marianecci C, & Carafa M. (2021). Hyaluronic Acid Derivative Effect on Niosomal Coating and Interaction with Cellular Mimetic Membranes. Molecules, 26(11), 3434.
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9

Biophysical Characterization of Natural Oils

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Almond oil was purchased by Farmalabor Srl (Assago (MI) Italy). Neem oil was purchased by Neem Italia (Moniga del Garda (BS), Italy) and characterized by an ECOCERT certificate (Biocert Italia IT013BC041 – ICEA 264BC001). Polysorbate 80‎ (Tw80), tocopherol (vitamin E), Hepes salt {N-(2-hydroxyethyl), piperazine-N-(2-ethanesulfonic acid)}, 1.6-Diphenyl-1,3,5-hexatriene (DPH) and pyrene were Sigma-Aldrich products (Sigma-Aldrich, Milan, Italy). Ethanol and all other products and reagents were of analytical grade.
Rinaldi F., Hanieh P.N., Maurizi L., Longhi C., Uccelletti D., Schifano E., Del Favero E., Cantù L., Ricci C., Ammendolia M.G., Paolino D., Froiio F., Marianecci C, & Carafa M. (2022). Neem Oil or Almond Oil Nanoemulsions for Vitamin E Delivery: From Structural Evaluation to in vivo Assessment of Antioxidant and Anti-Inflammatory Activity. International Journal of Nanomedicine, 17, 6447-6465.
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10

Neem Oil Characterization and Application

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Neem oil was purchased by Neem Italia (Moniga del Garda (BS), Italy) and is characterised by an ECOCERT certificate (Biocert Italia IT013BC041 – ICEA 264BC001). Tween 20 (Tw20), Hepes salt {N-(2-hydroxyethyl) piperazine-N-(2-ethanesulphonic acid)} and pyrene were Sigma-Aldrich products (Sigma-Aldrich, Milan, Italy). Distilled water (Ecological Product System s.r.l., Rome, Italy) was used for all experiments. All other products and reagents were of analytical grade.
Rinaldi F., Hanieh P.N., Longhi C., Carradori S., Secci D., Zengin G., Ammendolia M.G., Mattia E., Del Favero E., Marianecci C, & Carafa M. (2017). Neem oil nanoemulsions: characterisation and antioxidant activity. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 1265-1273.
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