ELISA plate-bound Phl p 7 (0.1 μg/well) was pre-incubated with rabbit antisera (1:20 in buffer B) raised against Phl p 7, Phl p 7 peptides 1 or 2 (Fig. 4A ) (inhibitors), or a normal rabbit serum (control). After washing, mAb102.1F10 or control IgG4 (Sigma-Aldrich) (0.1 μg/well) was added and bound human IgG antibodies were detected with HRP-labelled rabbit anti-human IgG antibodies (Dako). All determinations were performed in duplicates, mean ODs were calculated and percent inhibitions were calculated according to the formula: 100 – (OD mAb102.1F10-binding after pre-incubation with inhibitors* 100/ OD mAb102.1F10–binding after pre-incubation with control) (Fig. 4B ).
Hrp labelled rabbit anti human igg antibodies
Manufactured by Agilent Technologies
HRP-labelled rabbit anti-human IgG antibodies are a type of laboratory reagent used in immunoassays. They consist of rabbit-derived antibodies that specifically bind to human immunoglobulin G (IgG) molecules and are conjugated with the enzyme horseradish peroxidase (HRP).
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Lab products found in correlation
2 protocols using hrp labelled rabbit anti human igg antibodies
1
Competitive Binding Assay for Phl p 7
2
Inhibition of Phl p 7 Binding by Antibodies
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ELISA plate‐bound Phl p 7 (0.1 μg/well) was pre‐incubated with rabbit antisera (1 : 20 in buffer B) raised against Phl p 7, Phl p 7 peptides 1 or 2 (Fig. 4 A) (inhibitors), or a normal rabbit serum (control). After washing, mAb102.1F10 or control IgG4 (Sigma‐Aldrich) (0.1 μg/well) was added and bound human IgG antibodies were detected with HRP‐labelled rabbit anti‐human IgG antibodies (Dako). All determinations were performed in duplicates, mean ODs were calculated, and percentage inhibitions were calculated according to the formula: 100 – (OD mAb102.1F10 binding after pre‐incubation with inhibitors * 100/OD mAb102.1F10 binding after pre‐incubation with control) (Fig. 4 B).
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