For live imaging, larvae were anesthetized with a 610 μM solution of the anesthetic 3-aminobenzoic acid ethyl ester (MS-222) and mounted onto a glass-bottom 3-cm Petri dish (MatTek) and covered with 1% low-melting-point agarose with diluted anesthetic. Images were acquired with an inverted laser-scanning confocal microscope, or an inverted spinning-disc confocal microscope (Visitron), with a 40× air or 63× water-immersion objective. z-stacks of Schwann cells and axons consisted of 1-μm-spaced images, which were analyzed with ImageJ software.
Glass bottom 3 cm petri dish
Manufactured by MatTek
Sourced in United States
The Glass-bottom 3-cm Petri dish is a versatile laboratory equipment used for various cell culture applications. It provides a transparent glass surface for direct microscopic observation of cells and tissues. The dish measures 3 centimeters in diameter and is designed to maintain a sterile environment for cell growth and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using glass bottom 3 cm petri dish
1
In Vivo Imaging of Schwann Cells and Axons
2
Zebrafish Larvae Imaging and Mounting
3
Tracking Cell Lineage in Larval Regeneration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Larvæ were anesthetized, mounted onto a glass-bottom 3 cm Petri dish (MatTek) and covered with 1% low-melting point agarose with diluted anesthetic. Z-stack series were acquired every 15 min at 28.5°C using a 63X water-immersion objective. Cells were tracked overtime using volumetric Z-stack images with FIJI plugin MTrackJ (Meijering et al., 2012 (link)). Movies were registered two times for image stabilization and centered upon the centroid of the surviving group of cells and the subsequent regenerating organs. Founder cells are identified from 1 to 6 (n) and their daughter cells receive 2 n and 2n + 1 identities. All images were processed with the FIJI software package.
4
Vital Imaging of Zebrafish Mechanoreceptors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For vital imaging, zebrafish larvae at 6 dpf were anesthetized with a 610 µM solution of the anaesthetic 3-aminobenzoic acid ethyl ester (MS-222). Mechanoreceptive hair cells were identified by labeling with the vital dye Di-2-ASP (Molecular Probes, Eugene, OR USA) or by EGFP expression in transgenic fish. Samples were mounted onto a glass-bottom 3 cm Petri dish (MatTek, Ashland, MA USA) and covered with 1% low-melting-point agarose with diluted anaesthetic. Images were acquired with an inverted confocal microscope with a 40× air or 63× water-immersion objective lenses.
5
Zebrafish Larval Mounting for Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Zebrafish larvae were anaesthetized with 0.2 mg ml−1 of 3-aminobenzoic acid ethyl ester (MS-222). For confocal microscopy, animals were mounted ventral side up in 1% ultra-pure low-melting-point agarose (Invitrogen) onto a glass-bottom 3-cm Petri dish (MatTek). For two-photon microscopy, embryos were mounted ventral side up in low-melting-point agarose on a glass coverslip. The coverslip was then flipped over on a glass slide with a ring of high-vacuum grease filled with a drop of 0.2 mg ml−1 of MS-222 to prevent drying out of the agarose. After imaging, the animals were either euthanized or released from the agarose using microsurgery forceps and kept individually until further use.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!