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Cd86 pb

Manufactured by BioLegend
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Sourced in Austria

CD86-PB is a fluorescently-labeled antibody product from BioLegend. It is designed for the detection of the CD86 protein, which is a co-stimulatory molecule expressed on antigen-presenting cells.

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Lab products found in correlation

Cd11c pe cy7, by BD (1 mentions) Cd69 pe cy7, by BD (1 mentions) Cd19 apc cy7, by BD (1 mentions) Facsverse, by BD (1 mentions) Cd4 v500, by BD (1 mentions) Cd23 pe cy7, by Thermo Fisher Scientific (1 mentions) Cd19 v450, by BD (1 mentions) Igm percp cy5, by BD (1 mentions) Cd45.2 v500, by BD (1 mentions) Cd45.2 apc, by BD (1 mentions) Igd pe, by Thermo Fisher Scientific (1 mentions) Cd45.1 fitc, by Thermo Fisher Scientific (1 mentions) Cd11b percp cy5, by Thermo Fisher Scientific (1 mentions) Aa4.1 apc, by Thermo Fisher Scientific (1 mentions) Streptavidin percp, by BD (1 mentions) Igg1 fitc, by BD (1 mentions) Cd40 apc, by Thermo Fisher Scientific (1 mentions) Cd14 pe, by BD (1 mentions) Hla dr apc cy7, by BioLegend (1 mentions) Anti ifn γ fitc, by BD (1 mentions)

3 protocols using cd86 pb

1

Comprehensive Immune Cell Profiling

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The following antibodies were used: CD4-V500, CD11c-PE-Cy7, CD19-V450, CD19-APC-Cy7, CD69-PE-Cy7, IgM-PerCP-Cy5.5, CD45.2-V500, CD45.2-APC (BD Biosciences), IgD-PE, CD45.1-FITC, CD11b-PerCP-Cy5.5, CD21-PB, CD23-PE-Cy7, AA4.1-APC (eBiosciences), and CD86-PB, CD45.1-PB (BioLegend). Following red blood cell lysis, Fc receptor blockade, and staining, cells were processed on a BD FACSVerse or LSR II flow cytometer and analyzed using FlowJo v9.6.4 (Treestar).
Mills R.E., Lam V.C., Tan A., Cresalia N., Oksenberg N., Zikherman J., Anderson M., Weiss A, & Hermiston M.L. (2015). Unbiased modifier screen reveals that signal strength determines the regulatory role murine TLR9 plays in autoantibody production. Journal of immunology (Baltimore, Md. : 1950), 194(8), 3675-3686.
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2

Multi-color Flow Cytometry of Immune Cells

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The following mAbs and reagents were used: IgG1-FITC, IgG1-PerCP-Cy5.5, IgG2a-APC, IgG1-PB, CD14-PE, CD14-PErCP, CD45-HorizonV500, streptavidin-PerCP, anti-IFNγ-FITC, 7AAD and Lineage cocktail-FITC (CD3, 16,19, 20,14, 56) from BD Biosciences, Erembodegem, Belgium; CD80-FITC from Beckman Coulter, Woerden, the Netherlands; anti-BDCA1-PE, anti-BDCA2-FITC, anti-BDCA2-biotin, anti-BDCA4-PE, anti-BDCA3-APC, anti-Clec9a-VioBlue, CD19 microbeads and anti-PE-microbeads from Miltenyi Biotec, Bergisch Gladbach, Germany; CD1a-PB, CD209 (DC-SIGN)-PECy7, CD206-eFluor450, CD40-APC and CD274 (PDL1)-PECy7 from eBioscience, Vienna, Austria; CD86-APC, CD86-PB, CD163-PerCP-Cy5.5, CD40-PerCP-Cy5.5, HLA DR-APC-Cy7 and CD209 (DC-SIGN)-APC from Biolegend, London, United Kingdom. PMA, ionomycin and brefeldin A are from Sigma-Aldrich, St Louis, MO. The fix&perm cell permeabilization kit was obtained from An der Grub, Vienna, Austria.
Boor P.P., Bosma B.M., Tran K.T., van der Laan L.J., Hagenaars H., IJzermans J.N., Metselaar H.J, & Kwekkeboom J. (2019). Characterization of Antigen-Presenting Cell Subsets in Human Liver-Draining Lymph Nodes. Frontiers in Immunology, 10, 441.
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3

Immunophenotyping and ELISA Assays for Vaccine Evaluation

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Cells were stained with fluorophore-labeled antibodies and fixed with 4% paraformaldehyde before analysis with a BD FACSCalibur or LSRII. The following antibodies from BioLegend were used for flow cytometry: CD45-A700, CD69-FITC/-PB, CD80-FITC, NK1.1-PE, CD11b-FITC, CD11c-PE, B220-PerCP-Cy5.5, I-A/I-E-PerCP-Cy5.5, Ly6G-PE, Ly6C-APC, CD3-APC/-FITC, CD40-APC, PD-L1-APC, IgG2b-κ-APC, CD45-A700, CD4-APC/-PB, CD86-PB, and CD8-PerCP-Cy5.5/-BV711. APC-labeled H-2K(b) SIINFEKL tetramer was from the NIH. IFNβ levels in cell-culture media and mouse sera were measured with an IFNβ enzyme-linked immunosorbent assay (ELISA) kit (Invivogen) according to the manufacturer’s instructions. For anti-OVA IgG ELISA, 96-well ELISA plates (Greiner Bio-One) were coated with 10 μg/mL of OVA (Sigma) and incubated with diluted sera. These plates were incubated with HRP-conjugated anti-mouse IgG (Cell Signaling), and the optical density (OD) at 450 nm was measured after developing with 3,3′,5,5′-tetramethylbenzidine substrate (Thermo Scientific).
Yum S., Li M., Fang Y, & Chen Z.J. (2021). TBK1 recruitment to STING activates both IRF3 and NF-κB that mediate immune defense against tumors and viral infections. Proceedings of the National Academy of Sciences of the United States of America, 118(14), e2100225118.
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