Mouse limk1

Manufactured by Abcam

Sourced in United States

Mouse LIMK1 is a recombinant protein that corresponds to the full-length sequence of the mouse LIM domain kinase 1 (LIMK1) enzyme. LIMK1 is a serine/threonine protein kinase that regulates actin cytoskeleton dynamics through the phosphorylation of cofilin. The core function of this protein is to serve as a research tool for the study of LIMK1 and its role in cellular processes.

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Lab products found in correlation

Rabbit lrrk2 mjff2, by Abcam (2 mentions) Rabbit lrrk2 phospho s935, by Abcam (2 mentions) Rabbit lrrk2 phospho t2483, by Abcam (2 mentions) Rabbit lrrk2 phospho t1491, by Abcam (2 mentions) Mouse c myc 9e10, by Roche (2 mentions) Rabbit phospho pak4 5 6, by Merck Group (2 mentions) Rabbit lrrk2 udd3, by Abcam (2 mentions) Mouse c myc, by Roche (2 mentions)

Close spelling variants of this product

LIMK1 (3 citations)

2 protocols using mouse limk1

Immunoblotting Analysis of LRRK2 Signaling

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For immunoblotting analysis the following antibodies were used: rabbit LRRK2 MJFF2 (Epitomics Cat# 3514-1, RRID:AB_10643781, 1:100), rabbit LRRK2 phospho-S935 (Epitomics Cat# 5099-1, RRID:AB_ 11132319, 1:100), rabbit LRRK2 phospho-T2483 (Abcam Cat#156577, 1:2000), rabbit LRRK2 phospho-T1491 (Abcam Cat#140106, 1:2000), mouse Flag M2 (Sigma, Cat# F1804, 1:10000), mouse c-Myc 9E10 (Roche, Cat# 11667149001, RRID:AB_ 390912, 1:5000), rabbit PAK6 (Prestige® Sigma, Cat# HPA031124, RRID:AB_10601044, 1:2000), mouse β-tubulin (Sigma, Cat# T8328, RRID:AB_1844090, 1:5000), rabbit phospho-PAK4-5-6 (Sigma, Cat# SAB4504052, 1:2000), rabbit phospho-LIMK1 (Cell Signaling, Cat#3841, 1:1000), mouse LIMK1 (Abcam, Cat#117623, 1:1000).
For immunoprecipitation, the following antibodies were used: rabbit LRRK2 UDD3 (Epitomics, Cat# 5097-1, 1 μg/mg total proteins), mouse c-Myc (9E10, Roche, Cat# 11667149001, RRID:AB_390912, 0.8 μg/mg total proteins), mouse Flag M2 (Sigma, Cat# F1804, 1 μg/mg total proteins).

Civiero L., Cirnaru M.D., Beilina A., Rodella U., Russo I., Belluzzi E., Lobbestael E., Reyniers L., Hondhamuni G., Lewis P.A., Van den Haute C., Baekelandt V., Bandopadhyay R., Bubacco L., Piccoli G., Cookson M.R., Taymans J, & Greggio E. (2015). Leucine‐rich repeat kinase 2 interacts with p21‐activated kinase 6 to control neurite complexity in mammalian brain. Journal of Neurochemistry, 135(6), 1242-1256.

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Immunoblotting Analysis of LRRK2 Phosphorylation

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunoblotting analysis the following antibodies were used: rabbit LRRK2 MJFF2 (Cat# 3514‐1, RRID:AB_10643781, 1 : 100; Epitomics), rabbit LRRK2 phospho‐S935 (Cat# 5099‐1, RRID:AB_ 11132319, 1 : 100; Epitomics, Cambridge, UK), rabbit LRRK2 phospho‐T2483 (Cat#156577, 1 : 2000; Abcam, Cambridge, UK), rabbit LRRK2 phospho‐T1491 (Cat#140106, 1 : 2000; Abcam,Cambridge, UK), mouse Flag M2 (Cat# F1804, 1 : 10000; Sigma), mouse c‐Myc 9E10 (Cat# 11667149001, RRID:AB_ 390912, 1 : 5000; Roche Molecular Biochemicals, Indianapolis, IN, USA), rabbit PAK6 (Cat# HPA031124, RRID:AB_10601044, 1 : 2000, Prestige^®; Sigma), mouse β‐tubulin (Cat# T8328, RRID:AB_1844090, 1 : 5000; Sigma), rabbit phospho‐PAK4‐5‐6 (Cat# SAB4504052, 1 : 2000; Sigma), rabbit phospho‐LIM domain kinase 1 (LIMK1) (Cat#3841, 1 : 1000; Cell Signaling Technology, Beverly, MA, USA), mouse LIMK1 (Cat#117623, 1 : 1000; Abcam).
For immunoprecipitation, the following antibodies were used: rabbit LRRK2 UDD3 (Cat# 5097‐1, 1 μg/mg total proteins; Epitomics), mouse c‐Myc (9E10, Cat# 11667149001, RRID:AB_390912, 0.8 μg/mg total proteins; Roche), mouse Flag M2 (Cat# F1804, 1 μg/mg total proteins; Sigma).

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