Log In Sign up
The largest database of trusted experimental protocols

3 phosphoadenosine 5 phosphosulfate

Manufactured by Merck Group
Visit Supplier
Sourced in United States

3'-phosphoadenosine-5'-phosphosulfate is a chemical compound used in various laboratory and research applications. It serves as a sulfate donor in enzymatic reactions.

Automatically generated - may contain errors

Lab products found in correlation

Dithiothreitol (dtt), by Merck Group (3 mentions) Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) Adenosine 5 triphosphate, by Merck Group (2 mentions) N 2 hydroxylpiperazine n 2 ethanesulfonic acid hepes, by Merck Group (2 mentions) Ecolume scintillation cocktail, by MP Biomedicals (2 mentions) Carrier free sodium 35s sulfate, by American Radiolabeled Chemicals (2 mentions) Mgcl2, by Merck Group (2 mentions) Alamethicin, by Merck Group (2 mentions) Penicillin g, by Merck Group (1 mentions) Streptomycin sulfate, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Trizma base, by Merck Group (1 mentions) 2 morpholinoethane sulfonic acid mes, by Merck Group (1 mentions) Silica gel thin layer chromatography, by Merck Group (1 mentions) 6 oh mel, by LGC (1 mentions) Ultrafree mc 5000 nmwl filter units, by Merck Group (1 mentions) Hepg2 human hepatoma cells atcc hb 8065, by American Type Culture Collection (1 mentions) Caco 2 human colon adenocarcinoma cells atcc htb 37, by American Type Culture Collection (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

3’-phosphoadenosine-5’-phosphosulfate (PAPS), (16 citations)

9 protocols using 3 phosphoadenosine 5 phosphosulfate

1

In Vitro Sulfation Assay for Xenobiotics

Check if the same lab product or an alternative is used in the 5 most similar protocols
6-OH-Mel, NAS and 4-OH-Ram were products of Toronto Research Chemicals (Toronto, Ontario, Canada). Adenosine 5’-triphosphate (ATP), 3’-phosphoadenosine 5’-phosphosulfate (PAPS), 2-morpholinoethanesulfonic acid (MES), N-2-hydroxylpiperazine-N’-2-ethanesulfonic acid (HEPES), 3-N-tris-hydroxymethyl methylamino-propanesulfonic acid (TAPS), 2-cyclohexylamino ethanesulfonic acid (CHES), 3-cyclohexylamino-1-propanesulfonic acid (CAPS), Trizma base, dithiothreitol (DTT), minimum essential medium (MEM), fetal bovine serum (FBS), penicillin G, streptomycin sulfate, and silica gel thin-layer chromatography (TLC) plates were from Sigma Chemical Company (St. Louis, MO, USA). Carrier-free sodium [35S]sulfate was from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA); and Ecolume scintillation cocktail was from MP Biomedicals (Irvine, CA, USA). Ultra free-MC 5000 NMWL filter units were products of Millipore Corporation (Bedford, MA, USA). HepG2 human hepatoma cells (ATCC HB-8065) and Caco-2 human colon adenocarcinoma cells (ATCC HTB-37) were obtained from American Type Culture Collection (Manassas, VA, USA). Pooled human lung, liver, small intestine, and kidney cytosols were purchased from XenoTech, LLC (Lenexa, KS, USA). All other chemicals used were of the highest grade commercially available.
Luo L., Zhou C., Kurogi K., Sakakibara Y., Suiko M, & Liu M.C. (2015). Sulfation of 6-hydroxymelatonin, N-acetylserotonin and 4-hydroxyramelteon by the human cytosolic sulfotransferases (SULTs). Xenobiotica; the fate of foreign compounds in biological systems, 46(7), 612-619.
+ Open protocol
+ Expand
2

Reagents for Cell Culture Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bradford assay reagent was purchased from Bio-Rad Laboratories, Inc. (Mississauga, ON, Canada). Alamethicin and UDP-glucuronic acid (UDPGA) were obtained from Corning Life Sciences (Tewkbury, MA, USA). Dulbecco’s Modified Eagle’s Medium (DMEM), F12/DMEM, fetal bovine serum (FBS), L-glutamine, N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES), penicillin–streptomycin (Pen-strep) solution, TryPLE Express, and trypsin [0.25% with EDTA (1 ×)] were obtained from Life Technologies Inc. (Burlington, ON, Canada). Docosahexaenoic acid (DHA) was purchased from Nu-Chek Prep Inc (Elysian, MN, USA). Human epidermal growth factor (EGF) was purchased from PeproTech (Rocky Hill, NJ, USA). NADPH was obtained from Roche Diagnostics (Laval, QC, Canada). Bovine insulin, bovine serum albumin (BSA), hydrocortisone, 3′-phosphoadenosine-5′-phosphosulfate (PAPS), phloridzin (PZ), quercetin, S-adenosylmethionine (SAM) were purchased from Sigma-Aldrich (Oakville, ON, Canada).
Fernando W., Goralski K.B., Hoskin D.W, & Rupasinghe H.P. (2020). Metabolism and pharmacokinetics of a novel polyphenol fatty acid ester phloridzin docosahexaenoate in Balb/c female mice. Scientific Reports, 10, 21391.
+ Open protocol
+ Expand
3

Enzymatic Synthesis and Purification of ε-Viniferin

Check if the same lab product or an alternative is used in the 5 most similar protocols
ε-Viniferin was produced and purified in our laboratory (Unité de recherché Œnologie—EA 4577, Villenave d’Ornon, France). Pyridine, chlorosulfonic acid, acetobromo-glucuronic acid methyl-ester, sodium hydroxide (NaOH), uridine 5′-diphospho-glucuronic acid trisodium salt (UDPGA), alamethicin, 3′-phosphoadenosine-5′-phosphosulfate (PAPS), dithiothreitol (DTT), trifluoroacetic acid (TFA), formic acid (FA), and MgCl2 were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). Human liver S9 and rat liver S9 fractions were purchased from Biopredic International (Saint-Grégoire, France). Acetonitrile and methanol were purchased from Thermo Fisher Scientific (Illkirch, France).
Courtois A., Jourdes M., Dupin A., Lapèze C., Renouf E., Biais B., Teissedre P.L., Mérillon J.M., Richard T, & Krisa S. (2017). In Vitro Glucuronidation and Sulfation of ε-Viniferin, a Resveratrol Dimer, in Humans and Rats. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(5), 733.
+ Open protocol
+ Expand
4

Synthesis and Characterization of Raloxifene Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols
Raloxifene hydrochloride, raloxifene-6-glucuronide, and raloxifene-4′-glucuronide were purchased from Toronto Research Chemicals (Toronto, Canada, all compounds purity ≥99%). Raloxifene-6-sulfate was synthesized in our laboratory using rat and mouse liver S9 fraction, the purity of R-6-S was over 95% by ultra-performance liquid chromatography-UV. 3′-Phosphoa-denosine-5′-phosphosulfate and sulfatase (without α-glucuronidase) were purchased from Sigma-Aldrich (St. Louis, MO). The MS grade water, methanol, and acetonitrile were obtained from EMD (Gibbstown, NJ, USA).
Du T., Sun R., Li L., Ebuzoeme C., Bui D., Zheng Z., Yin T., Liang D., Hu M, & Gao S. (2020). Development and validation of ultra-high-performance liquid chromatography–mass spectrometry method for the determination of raloxifene and its phase II metabolites in plasma: Application to pharmacokinetic studies in rats. Journal of separation science, 43(24), 4414-4423.
+ Open protocol
+ Expand
5

Synthesis and Analysis of PAP[35S]

Check if the same lab product or an alternative is used in the 5 most similar protocols
E2 and 4OH-tamoxifen were products of Cayman Chemical Company (Ann Arbor, MI, USA). Diethylstilbestrol, adenosine 5’-triphosphate (ATP), dimethyl sulfoxide (DMSO), dithiothreitol (DTT), 3’-phosphoadenosine-5’-phosphosulfate (PAPS), isopropyl-1-thio-β-D-galactopyranoside (IPTG), and N-2-hydroxylpiperazine-N’-2-ethanesulfonic acid (HEPES) were from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). Ecolume scintillation cocktail was obtained from MP Biomedicals (Solon, OH, USA). Carrier-free sodium [35S]sulfate was from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA). Recombinant human bifunctional ATP sulfulyase/adenosine 5’-phosphosulfate kinase was prepared as previously described [34 (link)], and used to synthesize PAP[35S] from ATP and [35S]sulfate based on an established procedure [34 (link)]. Cellulose thin-layer chromatography (TLC) plates were products of Macherey-Nagel (Düren, Germany). Dpn I was purchased from New England BioLabs (Ipswich, MA, USA). PrimeStar Max DNA polymerase was a product of Takara Bio Inc. (Mountain View, CA, USA). All blue prestained protein markers were obtained from BioLand Scientific LLC. (Paramount, CA, USA). Oligonucleotide primers were synthesized by Eurofins Genomics (Louisville, KY, USA). All other reagents and chemicals used were of the highest grades commercially available.
El Daibani A.A., Alherz F.A., Abunnaja M.S., Bairam A.F., Rasool M.I., Kurogi K, & Liu M.C. (2021). Impact of Human SULT1E1 Polymorphisms on the Sulfation of 17β-Estradiol, 4-Hydroxytamoxifen and Diethylstilbestrol by SULT1E1 Allozymes. European journal of drug metabolism and pharmacokinetics, 46(1), 105-118.
+ Open protocol
+ Expand
6

Evaluation of DPP-4 Inhibitor Metabolism

Check if the same lab product or an alternative is used in the 5 most similar protocols
Saxagliptin, trelagliptin, and gemigliptin (Figure 1) were purchased from AdooQ Bioscience (>98%, Irvine, CA, USA); linagliptin and 5-hydroxySaxagliptin from Toronto Research Chemicals (Toronto, Canada); ammonium acetate (99.99%), β-nicotinamide adenine dinucleotide phosphate sodium salt hydrate (NADP+), dimethyl sulfoxide (DMSO, >99.7%), glucose-6-phosphate (G6P), glucose-6-phosphate dehydrogenase (G6PD), L-cysteine, magnesium chloride, reduced glutathione (GSH, ≥99%), 3′-phosphoadenosine-5′-phosphosulfate (PAPS), potassium phosphate, uridine diphosphate glucuronic acid (UDPGA, >98%), and vildagliptin from Sigma-Aldrich (St. Louis, MO, USA); and rat liver microsomes (RLM, R1000) from XenoTech (Lenexa, KS, USA). All solvents were LC-MS grade from Fisher Scientific (Pittsburgh, PA, USA).
Kim K.Y., Jeong Y.J., Park S.Y., Park E.J., Jeon J.H., Song I.S, & Liu K.H. (2024). Evaluation of the Drug-Induced Liver Injury Potential of Saxagliptin through Reactive Metabolite Identification in Rats. Pharmaceutics, 16(1), 106.
+ Open protocol
+ Expand
7

Radiochemical Assay for Estrogen Sulfotransferase

Check if the same lab product or an alternative is used in the 5 most similar protocols
EST activities were measured using a radiochemical extraction assay35 (link). 3′-Phosphoadenosine-5′-phosphosulfate was purchased from Sigma-Aldrich chemical company (St. Louis, MO), and 3H-estradiol (60 Ci/mmol) was purchased from PerkinElmer Life Sciences Inc. (Boston, MA). Incubation mixtures (0.5 ml) contained 100 mM Tris–HCl (pH 7.4), 5 mM MgCl2, 0.8 mg placental cytosolic protein, 20 μM PAPS, and 2 nM [3H] E2. The reaction was initiated with the addition of PAPS and continued for 30 minutes at 37 °C. The reaction mixture was extracted with 1 ml of dichloromethane and aliquot of the aqueous phase was counted by liquid scintillation counting.
Wang X., Chen X., Feng X., Chang F., Chen M., Xia Y, & Chen L. (2015). Triclosan causes spontaneous abortion accompanied by decline of estrogen sulfotransferase activity in humans and mice. Scientific Reports, 5, 18252.
+ Open protocol
+ Expand
8

Chlorinated Paraffin Biotransformation Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercial SCCP mixtures (Cl degree: 55.5%), MCCP mixtures (Cl degree: 52%), and LCCP mixtures (Cl degree: 49%) were purchased from Dr. Ehrenstorfer (Augsburg, Germany). 13 C-BDE 209 was purchased from Wellington Laboratories Inc (Guelph, ON, Canada). Pooled human liver microsomes (HLM), pooled human liver cytosol (HLCYT), 2,6-uridinediphosphate glucuronic acid (UDPGA), alamethicin (neat, purity > 99%), 3'-phosphoadenosine-5'-phosphosulfate (PAPS; purity > 60%) lithium salt hydrate, nicotinamide adenine dinucleotide phosphate (NADPH; purity > 99%), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Castle Hill, NSW, Australia). Ultrapure water was obtained from a Milli-Q system (Merck Millipore, MA, USA). Gas chromatography grade acetonitrile (ACN) was purchased from Merck (Darmstadt, Germany).
He C., van Mourik L., Tang S., Thai P., Wang X., Brandsma S.H., Leonards P.E.G., Thomas K.V, & Mueller J.F. (2021). In vitro biotransformation and evaluation of potential transformation products of chlorinated paraffins by high resolution accurate mass spectrometry. Journal of hazardous materials, 405.
+ Open protocol
+ Expand
9

Bisphenol A Bioactivation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bisphenol A (>99%; CAS 80-05-7), BPA sulfate, sodium salt (95%, CAS 847696-37-1), BPF (98%; CAS 620-92-8), BPS (98%; CAS 80-09-1), dithiothreitol (98%; CAS 3483-12-3), resazurin, sodium salt (80%; CAS 62758-13-8), MgCl 2 (≥98%; CAS 7786-30-3), 3 ′ -phosphoadenosine-5 ′phosphosulfate, and dimethyl sulfoxide were all from Sigma-Aldrich (St. Louis, MO, USA). 17-β-Estradiol (E2; ≥98%; CAS 50-28-2), 17-α-estradiol (≥98%; CAS 57-91-0), corticosterone (≥98.5%; CAS 50-22-6), and kanamycin solution from Streptomyces kanamyceticus (10 mg/mL; CAS 25389-94-0) were from Sigma-Aldrich (Germany). Pooled human liver cytosol (total protein concentration, 20 mg/mL) was from Thermo Fisher Scientific (MA, USA). Methyltestosterone (>98%; CAS 58-18-4) was from Tokyo Chemical Industry (TCI Europe, Belgium). Methanol and acetonitrile were LC-MS grade.
Durcik M., Gramec Skledar D., Tomašič T., Trontelj J, & Peterlin Mašič L. (2022). Last piece in the puzzle of bisphenols BPA, BPS and BPF metabolism: Kinetics of the in vitro sulfation reaction. Chemosphere, 303(Pt 2).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!