Digital sight ds 5m l1 camera

Manufactured by Nikon

Sourced in United States

The Nikon Digital Sight DS-5M-L1 is a high-resolution digital camera designed for use with microscopes. It features a 5-megapixel CMOS sensor and is capable of capturing images with a resolution of 2560 x 1920 pixels. The camera is equipped with a C-mount interface, allowing it to be easily attached to a variety of microscope systems.

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Lab products found in correlation

Eclipse 50i light microscope, by Nikon (2 mentions) Eclipse e400, by Nikon (1 mentions) Albumax 1, by Thermo Fisher Scientific (1 mentions) Eclipse e400 microscope, by Nikon (1 mentions) Anti cleaved casp3, by Cell Signaling Technology (1 mentions) Super block, by ScyTek Laboratories (1 mentions) Anti iba1, by Fujifilm (1 mentions) Goat anti rabbit igg h l alexa fluor 488, by Abcam (1 mentions) Bx 40, by Leica (1 mentions)

Close spelling variants of this product

Digital Sight DS-5M-L1 Digital Sight DS-5M camera Digital Sight DS-5M digital camera DS-5M-L1 digital camera Digital Sight DS-L1 camera Digital Sight DS-5M Digital Sight DS-L1 DS-5M-L1 DS-5M camera DS-L1 camera

4 protocols using digital sight ds 5m l1 camera

Maintenance of P. falciparum CS2 in Culture

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P. falciparum CS2 parasites were maintained in continuous culture according to routine methods^{27 (link)}. Parasites were cultured at 4% hematocrit in O+ RBCs. Cultures were maintained in 25 cm² or 75 cm² tissue culture flasks at 37°C under a gas mixture of 90% nitrogen/5% oxygen/5% carbon dioxide and in complete culture medium made up of RPMI containing 25 mM HEPES, 0.05 mg/mL hypoxanthine, 2.2 mg/mL NaHCO₃ (J.T. Baker), 0.5% Albumax I (Gibco), 2 g/L glucose, and 0.01 mg/mL gentamicin. Primarily ring-stage cultures were treated routinely with 5% D-Sorbitol to achieve synchronous cultures.
Culture health and stage of parasites were assessed daily and at each time point. Thin film blood smears were fixed with methanol, and stained with Giemsa for analysis by light microscopy. Images were acquired using a Nikon Eclipse E400 microscope fitted with a Nikon Digital Sight DS-5M-L1 camera (Nikon Instruments Inc.).

Chen F., Flaherty B.R., Cohen C.E., Peterson D.S, & Zhao Y. (2016). Direct Detection of Malaria Infected Red Blood Cells by Surface Enhanced Raman Spectroscopy. Nanomedicine : nanotechnology, biology, and medicine, 12(6), 1445-1451.

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Antimalarial Drug STAD-2 Evaluation

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Ring-stage or late-stage iRBC at <0.5% parasitemia were brought up to 4% hematocrit in complete culture medium and transferred to a 24-well tissue culture plate in 1 mL aliquots. iRBCs were then treated with 1 μM STAD-2, 1 μM scrambled STAD-2, or 0.001% DMSO (vehicle control). 25 μL were removed from each well every 24 hours post-treatment, and samples were stained with Hoechst 33342 and analyzed by flow cytometry. Blood smears were made every 24 hours, fixed with methanol, and stained with Giemsa for analysis by light microscopy. Images were acquired using a Nikon Eclipse E400 microscope fitted with a Nikon Digital Sight DS-5M-L1 camera (Nikon Instruments Inc.).

Flaherty B.R., Wang Y., Trope E.C., Ho T.G., Muralidharan V., Kennedy E.J, & Peterson D.S. (2015). The Stapled AKAP Disruptor Peptide STAD-2 Displays Antimalarial Activity through a PKA-Independent Mechanism. PLoS ONE, 10(5), e0129239.

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Immunofluorescent Analysis of Macrophages and Apoptosis in Kidney

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Kidney sections were immunolabeled with primary abs against macrophages, using the anti-Iba1 (cat#NCNP24, Fujifilm Wako, Pure Chemical Corporation, Tokyo, Japan) and anti-cleaved casp-3 (cat#9664, Cell Signaling Technology, Danvers, MA, USA) diluted 1:400 in Super Block (cat#AAA125, ScyTek Laboratories, Logan, UT, USA). The slides were incubated with fluorescent secondary Goat anti-rabbit IgG H&L (Alexa Fluor^® 488; cat#ab150077, Abcam, Cambridge, MA, USA) diluted 1:400 in blocking solution. The slides were washed and mounted with DAPI-containing mounting medium, covered with glass coverslips, and imaged on a Sight DS-5M-L1 digital camera (Nikon, Melville, NY, USA) connected to an Eclipse 50i light microscope (Nikon) at different magnifications. The images acquired were used for quantifications of Iba1⁺ cells and mean fluorescence intensity (MFI) for cleaved casp-3 in renal tubular cells, using Image J software (NIH).

Silva J.B., Calcia T.B., Silva C.P., Guilherme R.F., Almeida-Souza F., Lemos F.S., Calabrese K.S., Caruso-Neves C., Neves J.S, & Benjamim C.F. (2021). ATRvD1 Attenuates Renal Tubulointerstitial Injury Induced by Albumin Overload in Sepsis-Surviving Mice. International Journal of Molecular Sciences, 22(21), 11634.

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Histological Analysis of ZIKV-Induced Skeletal Muscle Pathology

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Skeletal muscle samples from mouse hind legs were collected bilaterally at birth or 6 dpi, fixed with 4% paraformaldehyde, and embedded in paraffin after dehydration. Paraffin-embedded tissue sections of 3 to 5 μm were prepared and stained with hematoxylin and eosin (H&E) or used for immunohistochemistry. H&E images were obtained by using optical microscopy at a magnification of 10× (Olympus BX40), and images were acquired using Leica Application Suite 3.8 software. For scoring, mock-infected or ZIKV-infected skeletal muscle tissues stained with H&E from at least five different animals were classified for the presence of necrosis, cellular infiltrate, and fiber atrophy by a researcher blind to the experimental condition, according to the following criteria: 0, absence; 1, some indicative; 2, moderate; 3, intense; and 4, very intense.
For immunohistochemistry of ZIKV dsRNA, sections of skeletal muscle were stained as previously described (8 (link)) with primary mouse anti-dsRNA antibodies (SCICONS J2) at a 1:200 dilution. Stained tissue sections were analyzed under identical conditions to allow comparison between immunoreactivity ODs. Slides were imaged on a Sight DS-5M-L1 digital camera (Nikon, Melville, NY) connected to an Eclipse 50i light microscope (Nikon).

Gavino-Leopoldino D., Figueiredo C.M., da Silva M.O., Barcellos L.G., Neris R.L., Pinto L.D., Araújo S.M., Ladislau L., Benjamim C.F., Da Poian A.T., Clarke J.R., Figueiredo C.P, & Assunção-Miranda I. (2021). Skeletal Muscle Is an Early Site of Zika Virus Replication and Injury, Which Impairs Myogenesis. Journal of Virology, 95(22), e00904-21.

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