Methanol, acetonitrile, formic acid, ammonium formate, and water (all LC/MS or Optima grade) were obtained from Fisher Chemicals (Fair Lawn, NJ, USA). Ammonium hydroxide (NH3 content 28–30%; analytical grade) was from Sigma-Aldrich (St. Louis, MO). Solid phase extraction (SPE) cartridges were 60-mg Oasis HLB with 30-µm particle size (Waters, Milford, MA). Borosilicate glass tubes, autosample vials, and polypropylene centrifuge tubes were purchased from Fisher Scientific. Milli Q water (>18 MΩ) was produced from a water purification system (Barnstead Nanopure). Multi-anion standards were from Sigma-Aldrich (St. Louis, MO, USA) and multi-cation standards were from Alltech (USA). Anatoxin-a, microcystin-LA, -LR, and -RR standards, as well as saxitoxin and cylindrospermopsin ELISA kits were all from Abraxis (Warminster, PA, USA). The 25-mm glass microfiber GF/A syringe filters were obtained from Whatman (GE Healthcare UK Limited, UK) and B-D 10-mL disposable syringes were from Becton Dickinson & Co (Franklin Lakes, NJ, USA). The IC eluents were sodium hydroxide (50%, w/w) for anion analysis and methanesulfonic acid for cation analysis.
Borosilicate glass tubes
Manufactured by Thermo Fisher Scientific
Borosilicate glass tubes are laboratory equipment designed for a variety of scientific applications. They are made from a specialized type of glass that is resistant to thermal shock and chemical corrosion, making them suitable for a wide range of experiments and procedures. These tubes provide a durable and reliable containment solution for liquids, gases, and other materials used in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Thermo Fisher Scientific (2 mentions)
Formic acid, by Thermo Fisher Scientific (2 mentions)
Ammonium formate, by Thermo Fisher Scientific (1 mentions)
Polypropylene centrifuge tubes, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Oasis hlb, by Waters Corporation (1 mentions)
Ammonium hydroxide, by Merck Group (1 mentions)
Porcine stomach mucin, by Merck Group (1 mentions)
Sodium thioglycolate, by Avantor (1 mentions)
Thc d3, by Cerilliant (1 mentions)
Cbn d3, by Cerilliant (1 mentions)
Ammonium acetate, by Thermo Fisher Scientific (1 mentions)
2 propanol, by Thermo Fisher Scientific (1 mentions)
11 oh thc, by Cerilliant (1 mentions)
Thccooh, by Cerilliant (1 mentions)
Thccooh gluc, by Cerilliant (1 mentions)
11 oh thc d3, by Cerilliant (1 mentions)
Cbd d3, by Cerilliant (1 mentions)
Optima lc ms grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Thca a, by Cerilliant (1 mentions)
3 protocols using borosilicate glass tubes
1
Analytical Protocol for Cyanotoxin Detection
2
Quantifying Clostridium perfringens Growth
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A total of 74 hyper-immune sera were developed against 37 different peptides. Each serum was tested in replicates of 5, with 3 replicate experiments, and all data are presented as a composite of replications. In sterile borosilicate glass tubes (12 × 75 mm; Fisher Scientific, Hampton, NH), 10 µ L of 1 × 106 CFU/mL of CP was added to 1 mL of 0.5X Luria-Bertani broth (Becton and Dickenson, Sparks, MD) containing 1.00% porcine stomach mucin (Sigma Aldrich, St. Louis, MO) and 20 µ L of chicken hyper-immune serum for a final concentration of 1 × 104 CFU/mL of CP in each tube. All tubes were then incubated in anaerobic jars at 37°C. At the 4, 6, and 8 h marks of incubation, samples were collected for quantification of CFU by serial dilution plating on tryptic soy agar (TSA ) with 0.25% sodium thioglycolate (VWR, Radnor, PA) and incubated in anaerobic jars at 37°C for 24 h. Growth (CFU/mL) of CP was calculated as a sample over negative ratio relative to the mean negative control and converted to a percentage.
3
Comprehensive Cannabinoid Standard Preparation
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Stocks of 1 mg/mL of CBD, THC, CBN, 11-OH-THC, CBG, THCV, THCA-A, and THCCOOH-D 3 as well as 100 μg/mL solutions of THCCOOH, THCCOOH-gluc, CBD-D 3 , THC-D 3 , CBN-D 3 , and 11-OH-THC-D 3 were purchased from Cerilliant (Round Rock, TX, USA). A 10-μg/mL THCgluc stock was obtained from ElSohly (Oxford, MS, USA).
Optima LC/MS grade acetonitrile, methanol, 2-propanol, formic acid (FA), and ammonium acetate were obtained from Fisher Scientific (Hampton, NH, USA). Oasis PRiME HLB 96-well solid-phase extraction (SPE) plates with 30 mg sorbent/well, 96-well collection plates, quick-load glass inserts, silicone/PTFE-treated 96-well square plug cap mats, and amber max recovery autosampler vials were acquired from Waters (Milford, MA, USA). Borosilicate glass tubes were from Fisher Scientific and 18.2 MΩ-cm water was dispensed from a Siemens PureLab Ultra purification system.
Blank WB was created by mixing acid citrate dextrose (ACD)packed red blood cells with ABO-compatible plasma and confirming the absence of detectable cannabinoids. Breath matrix consisted of an unused SensAbues ® electret filter material ("pad") cut into 28 cm 2 squares to match the intake surface area of the SensAbues ® collection device.
Optima LC/MS grade acetonitrile, methanol, 2-propanol, formic acid (FA), and ammonium acetate were obtained from Fisher Scientific (Hampton, NH, USA). Oasis PRiME HLB 96-well solid-phase extraction (SPE) plates with 30 mg sorbent/well, 96-well collection plates, quick-load glass inserts, silicone/PTFE-treated 96-well square plug cap mats, and amber max recovery autosampler vials were acquired from Waters (Milford, MA, USA). Borosilicate glass tubes were from Fisher Scientific and 18.2 MΩ-cm water was dispensed from a Siemens PureLab Ultra purification system.
Blank WB was created by mixing acid citrate dextrose (ACD)packed red blood cells with ABO-compatible plasma and confirming the absence of detectable cannabinoids. Breath matrix consisted of an unused SensAbues ® electret filter material ("pad") cut into 28 cm 2 squares to match the intake surface area of the SensAbues ® collection device.
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