Antibodies (Abs) used were: anti-CaV2.2 II-III loop Ab (rabbit polyclonal) (Raghib et al., 2001 (link)), anti-CaV2.1 II-III loop Ab (rabbit polyclonal, Alomone), anti- α2δ-1 Ab (mouse monoclonal, Sigma-Aldrich), anti-HA (rat monoclonal, Roche), anti-HA (rabbit polyclonal, Sigma), anti-PDI (mouse monoclonal, Abcam), anti-Akt Ab (rabbit polyclonal, Cell Signaling Technology) and anti-GAPDH Ab (mouse monoclonal, Ambion). For immunocytochemistry, secondary Abs (1:500) used were anti-rabbit-Alexa Fluor 594, anti-rat- Alexa Fluor 488, anti-rat-Alexa Fluor 594 and 647 and anti-mouse-Alexa Fluor 488 (ThermoFisher). For immunoblotting, secondary Abs (1:2000) were anti-rabbit-Horseradish Peroxidase (HRP), and anti-mouse HRP (Biorad).
Anti akt ab
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-Akt Ab is a laboratory reagent developed by Cell Signaling Technology. It is an antibody that specifically detects Akt, a serine/threonine protein kinase that plays a key role in cellular processes such as metabolism, proliferation, cell survival, and angiogenesis.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh ab, by Cell Signaling Technology (2 mentions)
Anti mouse alexa fluor 488, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 488 anti rat, by Thermo Fisher Scientific (1 mentions)
Anti α2δ 1 ab, by Merck Group (1 mentions)
Anti pdi, by Abcam (1 mentions)
Alexa fluor 594 anti rabbit, by Thermo Fisher Scientific (1 mentions)
Anti rabbit horseradish peroxidase hrp, by Bio-Rad (1 mentions)
Anti mouse hrp, by Bio-Rad (1 mentions)
Anti ha, by Roche (1 mentions)
Anti gapdh ab, by Thermo Fisher Scientific (1 mentions)
Anti ha, by Merck Group (1 mentions)
Mab006, by R&D Systems (1 mentions)
Anti pakt s473, by Cell Signaling Technology (1 mentions)
Anti erk1 2 ab, by Cell Signaling Technology (1 mentions)
Mab699, by R&D Systems (1 mentions)
Ikk 16, by Selleck Chemicals (1 mentions)
Anti erk ab, by Cell Signaling Technology (1 mentions)
Anti p38 ab, by Cell Signaling Technology (1 mentions)
Anti nf κb p65 ab, by Cell Signaling Technology (1 mentions)
Anti phospho nf κb p65 ab, by Cell Signaling Technology (1 mentions)
5 protocols using anti akt ab
1
Immunodetection of Ca2+ Channel Subunits
2
Antibody Sourcing and Validation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following antibodies (Abs) were purchased commercially: mouse anti–STAT3 Ab (9139, Cell signaling Technology), anti–Erk1/2 Ab (4696, Cell Signaling Technology), anti–Akt Ab (2920, Cell Signaling Technology) and anti–CXCR6 Ab (MAB699, R&D Systems); rabbit anti–Ror1 Ab (16 540 for western blot and 4102 for immunohistochemistry, Cell Signaling Technology), anti–α‐tubulin Ab (PM054, MBL), anti–pErk1/2 T202/Y204 Ab (4370, Cell Signaling Technology), anti–pAkt S473 (9271, Cell Signaling Technology) and anti–pSTAT3 S727 Ab (9134, Cell signaling Technology); rat anti–CXCL16 Ab (MAB976, R&D Systems) and control IgG (MAB006, R&D Systems).
3
Signaling Pathways Regulation in Cellular Processes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The primary Abs used included: anti‐TRAF6 Ab (Abcam), anti‐TRAF2 Ab (Abcam, Cambridge, MA, USA), anti‐AKT Ab (Cell Signaling Technology, Danvers, MA, USA), anti‐phospho‐AKT Ab (Thr308; Cell Signaling Technology), anti‐IκB kinase (IKK) Ab (Cell Signaling Technology), anti‐phospho‐IKKα/β Ab (Ser176/180; Cell Signaling Technology), anti‐NF‐κB p65 Ab (Cell Signaling Technology), anti‐phospho‐NF‐κB p65 Ab (Ser536; Cell Signaling Technology), anti‐IκBα Ab (Cell Signaling Technology), anti‐ phospho‐IκBα Ab (Ser32; Cell Signaling Technology), anti‐JNK Ab (Cell Signaling Technology), anti‐phospho‐JNK Ab (Thr183/Tyr185; Cell signaling), anti‐p38 Ab (Cell Signaling Technology), anti‐phospho‐p38 Ab (Thr180/Tyr182; Cell Signaling Technology), anti‐ERK Ab (Cell Signaling Technology), anti‐phospho‐ERK Ab (Thr202/Tyr204; Cell signaling), anti‐ubiquitin K63 Ab (Cell signaling), and anti‐GAPDH Ab (Cell signaling). The AKT inhibitor MK‐2206, the IKK inhibitor IKK‐16, and the proteasome inhibitor MG132 were purchased from Selleckchem (Houston, TX, USA).
4
Western Blot Analysis of Key Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blot analysis was performed as previously described.[9
] Primary antibodies included: mouse monoclonal anti‐CD133 (W6B3C1 clone) (Miltenyi Biotec), rabbit monoclonal anti‐GAPDH Ab (Cell signaling), anti‐Akt Ab (Cell signaling), anti‐p‐Akt (Thr308) Ab (Cell signaling), anti‐PI3‐kinase p85 Ab (Millipore), and anti‐SLC1A5 antibody (Cell signaling.
] Primary antibodies included: mouse monoclonal anti‐CD133 (W6B3C1 clone) (Miltenyi Biotec), rabbit monoclonal anti‐GAPDH Ab (Cell signaling), anti‐Akt Ab (Cell signaling), anti‐p‐Akt (Thr308) Ab (Cell signaling), anti‐PI3‐kinase p85 Ab (Millipore), and anti‐SLC1A5 antibody (Cell signaling.
5
Phosphorylation of Akt in ARPE-19 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ARPE-19 cells at 5×105 were incubated in the presence or absence of rIL-22 (10 ng/ml) for 5, 10, 20, and 30 min. The cells were then lysed, and the blocked membrane was incubated either with anti-pAkt Ab (1:1,000; Cell signaling Technology, Boston MA, USA), anti-Akt Ab (1:1,000; Cell signaling technology) or anti-β-actin (1:8,000; Sigma-Aldrich). The membrane was incubated with HRP-conjugated anti-rabbit IgG (1:15,000; Cell Signaling Technology) for p-Akt, Akt and HRP-conjugated anti-mouse IgG (1:10,000; Cell signaling technology) for β-actin for 1 h at RT.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!