Adenosine triphosphate (atp)

Manufactured by Bio-Techne

Sourced in United Kingdom

ATP is a laboratory product that functions as the primary energy currency in living cells. It is a nucleotide composed of an adenosine molecule and three phosphate groups. ATP plays a crucial role in various cellular processes, including energy transfer, signaling, and metabolic regulation.

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Lab products found in correlation

Pom 1, by Bio-Techne (2 mentions) Milli q biocel, by Merck Group (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Ficoll hypaque, by Cytiva (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) Ara c, by Merck Group (1 mentions) Minimacs high gradient magnetic separation column, by Miltenyi Biotec (1 mentions) Az10606120, by Bio-Techne (1 mentions) Acetylcholine ach, by Merck Group (1 mentions) L glutamate, by Merck Group (1 mentions) Bicuculline, by Bio-Techne (1 mentions) Geneticin, by Merck Group (1 mentions) Bumetanide, by Bio-Techne (1 mentions) Muscimol, by Bio-Techne (1 mentions) Picrotoxin, by Bio-Techne (1 mentions) Nifedipine, by Bio-Techne (1 mentions) Allopregnanolone, by Bio-Techne (1 mentions) Etomidate, by Bio-Techne (1 mentions) Benidipine, by Bio-Techne (1 mentions)

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Adenosine (7 citations)

6 protocols using adenosine triphosphate (atp)

AML Leukemia Cell Culture Protocol

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Primary leukemic cells were obtained from peripheral or bone marrow blood of 34 AML patients at diagnosis, before treatment (Supplementary Data, Supplementary Table S1). The percentage of leukemic blasts was always > 90%. Normal mononuclear cells (MNCs) were obtained from BM healthy donors. Mononuclear cells were isolated by Ficoll-Hypaque centrifugation (Amersham Bioscience, Piscataway, NJ, USA). In some experiments, normal PB MNCs were processed by MiniMacs high-gradient magnetic separation column (Miltenyi Biotec, Bergisch Gladbach, Germany) to obtain highly purified CD34⁺ cells.
For in vitro studies, cells were cultured in RPMI 1640 medium supplemented with 10% FBS (GIBCO, Thermo Fisher Scientific, MA, USA) in presence of increasing dose of ATP (Sigma Aldrich, Germany) and ARA-C (Sigma Aldrich). In some experiments, cells were pre-treated for 30 min at 37°C with 10 μM AZ 10606120, a P2×7R specific antagonist (Tocris, Bristol, United Kingdom).
The pH of ATP solution was adjusted to 6.8 using NaOH for both in vitro and in vivo experiments.
The research was approved by the Ethics Committee of Policlinico S.Orsola-Malpighi, University Hospital of Bologna and each individual gave written informed consent (Ethical Committee approval code: 147/2013/O/Tess).

Salvestrini V., Orecchioni S., Talarico G., Reggiani F., Mazzetti C., Bertolini F., Orioli E., Adinolfi E., Virgilio F.D., Pezzi A., Cavo M., Lemoli R.M, & Curti A. (2016). Extracellular ATP induces apoptosis through P2X7R activation in acute myeloid leukemia cells but not in normal hematopoietic stem cells. Oncotarget, 8(4), 5895-5908.

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Direct Receptor Activation of Mast Cells

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Direct activation of receptors on MCs was accomplished by repeated short applications (100 ms) of L-glutamate (1 mM, Sigma-Aldrich, St Louis, MO), acetylcholine (ACh, 1 mM, Sigma-Aldrich, St Louis, MO), ATP (1 mM, Tocris Bioscience, Minneapolis, MN), or ACSF to the IPL via a pipette that was similar to the recording pipette and a Picospritzer (PV830 Pneumatic PicoPump, World Precisions Instruments, Sarasota, FL) set at 20–30 psi.

Rosa J.M., Bos R., Sack G.S., Fortuny C., Agarwal A., Bergles D.E., Flannery J.G, & Feller M.B. (2015). Neuron-glia signaling in developing retina mediated by neurotransmitter spillover. eLife, 4, e09590.

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Investigating GABA Receptor Modulators

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Picrotoxin (50 μM), L-655 708 (11,12,13,13a-Tetrahydro-7-methoxy-9-oxo-9H-imidazo[1,5-a]pyrrolo[2,1 c][1,4]benzodiazepine-1-carboxylic acid, ethyl ester, 10 μM), SCS (Salicylidene salicylhydrazide, 1 μM), TPMPA ((1,2,5,6-Tetrahydropyridin-4-yl) methylphosphinic acid, 50 μM), etomidate (10 μM), allopregnanolone (100 nM), muscimol (5 μM), bumetanide (10 μM), nifedipine (10 μM), benidipine (10 μM), bay K 8644 (10 μM), bicuculline (50 μM), ATP (150 μM; all from Tocris Bioscience, Bristol, UK), SNAP ((S)-Nitroso-N-acetylpenicillamine, 50 μM), SC (semicarbazide 50 μM), GABA (5 μM), geneticin (10 μM; all from Sigma-Aldrich) and CPCPT (1-(3-Chlorophenethyl)−3-cyclopentylpyrimidine-2,4,6-(1H,3H,5H)-trione, 1 μM, Merck Millipore, Darmstadt, Germany) were used at the indicated concentrations, if not differently stated. All pharmacological treatments and live cell stainings were performed in CM at 37°C and 5% CO₂.

Bhandage A.K., Olivera G.C., Kanatani S., Thompson E., Loré K., Varas-Godoy M, & Barragan A. (2020). A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites. eLife, 9, e60528.

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Neurotransmitter Sensing Assay Protocol

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Adenosine and dopamine standards were purchased from Sigma Aldrich (St. Louis, MO) and ATP was purchased from Tocris Biosciences (Bristol, United Kingdom), and dissolved in 0.1 M HClO₄ for 10 mM stock solutions and diluted daily in Tris buffer for testing. 30 % hydrogen peroxide was purchased from Macron Fine Chemicals (Center Valley, PA) and diluted daily in Tris buffer to its final concentration. The Tris buffer solution consists of 15 mM Tris(hydroxymethyl)aminomethane, 1.25 mM NaH₂PO₄, 2.0 mM Na₂SO₄, 3.25 mM KCl, 140 mM NaCl, 1.2 mM CaCl₂ dehydrate, and 1.2 mM MgCl₂ hexahydrate at pH 7.4 (all Fisher, Suwanee, GA). For slice experiments, calibrations and training set solutions were performed in artificial cerebral spinal fluid (aCSF): 126 mM NaCl, 2.5 mMKCl, 1.2 mM NaH₂PO₄, 2.4 mM CaCl₂ dehydrate,1.2 mM MgCl₂ hexahydrate, 25 mM NaHCO₃, 11 mM glucose, and 15 mM tris (hydroxymethyl) aminomethane, pH 7.4 (all Fisher, Suwanne GA). POM-1 (sodium polyoxotungstate), an NTPDase 1,2 and 3 inhibitor, was purchased from Tocris. All aqueous solutions were made with deionized water (Milli-Q Biocel, Millipore, Billerica, MA).

Ross A.E, & Venton B.J. (2014). Sawhorse Waveform Voltammetry for Selective Detection of Adenosine, ATP, and Hydrogen Peroxide. Analytical Chemistry, 86(15), 7486-7493.

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Adenosine and Dopamine Assay Protocols

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Adenosine and dopamine
standards were purchased
from Sigma-Aldrich (St. Louis, MO) and ATP was purchased from Tocris
Biosciences (Bristol, United Kingdom) and dissolved in 0.1 M HClO₄ for 10 mM stock solutions and diluted daily in Tris buffer
for testing. Hydrogen peroxide (30 %) was purchased from Macron Fine
Chemicals (Center Valley, PA) and diluted daily in Tris buffer to
its final concentration. The Tris buffer solution consists of 15 mM
Tris(hydroxymethyl)aminomethane, 1.25 mM NaH₂PO₄, 2.0 mM Na₂SO₄, 3.25 mM KCl, 140 mM NaCl,
1.2 mM CaCl₂ dehydrate, and 1.2 mM MgCl₂ hexahydrate
at pH 7.4 (all Fisher, Suwanee, GA). For slice experiments, calibrations
and training set solutions were performed in artificial cerebral spinal
fluid (aCSF): 126 mM NaCl, 2.5 mM KCl, 1.2 mM NaH₂PO₄, 2.4 mM CaCl₂ dehydrate, 1.2 mM MgCl₂ hexahydrate, 25 mM NaHCO₃, 11 mM glucose, and 15 mM tris(hydroxymethyl)
aminomethane, pH 7.4 (all Fisher, Suwanne GA). POM-1 (sodium polyoxotungstate),
an NTPDase 1,2 and 3 inhibitor, was purchased from Tocris. All aqueous
solutions were made with deionized water (Milli-Q Biocel, Millipore,
Billerica, MA).

Ross A.E, & Venton B.J. (2014). Sawhorse Waveform Voltammetry for Selective Detection of Adenosine, ATP, and Hydrogen Peroxide. Analytical Chemistry, 86(15), 7486-7493.

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Chick Retina Isolation and Characterization

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All experiments involving animals were approved by and carried out in accordance with the guidelines of the Institutional Animal Care and Use Committee of the Federal University of Rio de Janeiro (permit number IBCCF-035), and all efforts were made to minimize suffering. Fertilized White Leghorn chicken eggs were obtained from a local hatchery. In this study, we used around 55 eggs (110 retinae). Embryos were staged according to [15 (link)] and sacrificed by decapitation on embryonic day 8 (E8). The eyes were removed and the retinas dissected out in a Ca²⁺- and Mg²⁺-free Hanks' (CMF) solution. We confirm that all animal use and experimental procedures were carried out in accordance with the guidelines of the Brazilian Society of Neuroscience and Behavior (SBNeC). Dulbecco's modified Eagle's medium (DMEM), fetal calf serum (FCS) and gentamycin were obtained from Gibco (USA). MK801 (Cat. No.0924) and DNQX were obtained from Tocris-Cookson (St. Louis, MO USA), ATP, propidium iodide, GABA, A-740003 (N-(1-{[(cyanoimino)(5-quinolinylamino) methyl] amino}-2,2-dimethylpropyl)-2-(3,4-dimethoxyphenyl)acetamide, Brilliant Blue G (BBG), thapsigargin, glutathione were from sigma. BAPTA-AM, CM-H2DCFDA and Fura-2 AM were obtained from Molecular Probes. [³H] GABA was from Amersham Pharmacia Biotech, Piscataway, NJ, USA. All other reagents were of analytical grade.

Freitas H.R., Ferraz G., Ferreira G.C., Ribeiro-Resende V.T., Chiarini L.B., do Nascimento J.L., Matos Oliveira K.R., Pereira T.D., Ferreira L.G., Kubrusly R.C., Faria R.X., Herculano A.M, & Reis R.A. (2016). Glutathione-Induced Calcium Shifts in Chick Retinal Glial Cells. PLoS ONE, 11(4), e0153677.

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