Anti bcl2 pe

Manufactured by BD

Anti-Bcl2 PE is a fluorochrome-conjugated antibody that binds to the Bcl-2 protein, which is a regulator of apoptosis (programmed cell death). This product is intended for use in flow cytometry applications to detect and quantify Bcl-2 expression in cells.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

PE-conjugated anti-human BCL2 (4 citations)

7 protocols using anti bcl2 pe

Multiparametric Flow Cytometry Analysis of T Cell Responses to TBEV

Check if the same lab product or an alternative is used in the 5 most similar protocols

T cell responses to TBEV were assessed using multi-color flow cytometry, and the monoclonal antibodies (mAbs) used were; anti-CD107a FITC, anti-CD4 Pacific Blue, anti-CD8 PerCP, anti-HLA-DR PerCP, anti-Ki67 FITC, anti-Ki67 Alexa Fluor 700, anti-Bcl2 PE, anti-CCR7 PE-Cy7, anti-MIP-1β PE, anti-CD14 BD horizon V500, anti-CD19 BD horizon V500, anti-perforin FITC and anti-granzyme B APC, anti-granzyme B PE-CF594 all from BD Biosciences (San Jose, CA). Anti-CD45RA APC-Cy7, anti TNF pacific blue, anti-IFN-γ Brilliant Violet 570, anti-CD27 Brilliant Violet 785, anti-CD27 Brilliant Violet 421, anti-Helios Pacific Blue, anti-T-bet Alexa Fluor 488, anti-T-bet PE-Cy7, anti-CCR7 Brilliant Violet 785, anti-CD279 Brilliant Violet 711, anti-CD27 biotin and anti-CD127 Brilliant Violet 570 were all from Biolegend (San Diego, CA). Anti-CD38 Alexa Fluor 700, anti-CD38 eFluor 650, anti-CD127 Alexa Fluor 780, anti-PD-1 (CD279) PE, anti-Eomes eFluor 660 and IgM eFluor 650 were all from eBioscience (San Diego, CA). Anti-CD4 Qdot 605, anti-CD8 Qdot705, anti-CD8 Qdot 605, Streptavidin-Qdot 585, anti-CD57 pure and Aqua Live/Dead were all from Invitrogen (Carlsbad, CA). Anti-CD3 ECD, anti-CD3 PE-Cy5, HLA-A2 CMV pp65 tetramer in PE and anti-CD56 ECD were from Beckman Coulter (Brea, CA). HLA-A2 ILLDNITTL tetramer in PE was kindly provided by the NIH Tetramer core facility.

Blom K., Braun M., Pakalniene J., Dailidyte L., Béziat V., Lampen M.H., Klingström J., Lagerqvist N., Kjerstadius T., Michaëlsson J., Lindquist L., Ljunggren H.G., Sandberg J.K., Mickiene A, & Gredmark-Russ S. (2015). Specificity and Dynamics of Effector and Memory CD8 T Cell Responses in Human Tick-Borne Encephalitis Virus Infection. PLoS Pathogens, 11(1), e1004622.

+ Open protocol

+ Expand

Multi-parameter Flow Cytometry Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-Bcl-2 PE, CD3-FITC, CD8-APC, CD127-BV510, IFNγ-APC, RORγt-BV421, TNF-PE were from BD Biosciences. Anti-CD4-PerCP, CD8-BV510, CD127-PECy7, CD161-BV421, -FITC, and -PE, IFNγ-APC, Ki-67-AF488 and -BV510, TNF-BV421, and Vα7.2 PECy7 were from Biolegend. Anti-PLZF APC was from R&D Systems. CellTrace Violet (CTV) cell proliferation kit, Live/Dead Aqua and Near Infrared fixable cell stain were from Life Technologies. MR1-5-OP-RU tetramer -APC, -BV421, and -PE were from NIH Tetramer Core Facility, Emory University. Staining with the MR1 5-OP-RU tetramers was performed for 40 min at room temperature (RT) (6 (link)) before proceeding to the surface and intracellular staining with other mAbs. Cell surface and intracellular staining for TFs, cytokines, and cytotoxic molecules were performed as previously described (10 (link)). Samples were acquired on an FACS Canto II (BD Biosciences) equipped with 405, 488, and 633 nm lasers or CytoFLEX (Beckman Coulter) equipped with 405, 488, and 638 nm lasers. Data including the compensation platform were analysed using FlowJo v.10 (BD Biosciences).

Han F., Gulam M.Y., Zheng Y., Zulhaimi N.S., Sia W.R., He D., Ho A., Hadadi L., Liu Z., Qin P., Lobie P.E., Kamarulzaman A., Wang L.F., Sandberg J.K., Lewin S.R., Rajasuriar R, & Leeansyah E. (2022). IL7RA single nucleotide polymorphisms are associated with the size and function of the MAIT cell population in treated HIV-1 infection. Frontiers in Immunology, 13, 985385.

+ Open protocol

+ Expand

Flow Cytometric Analysis of Phosphorylated Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used for flow cytometry analysis: Anti-Akt1-PE, anti-ERK1/2 (pT202/pY204)-PE, anti-STAT1 (pY701)-VioBlue and anti-CD34- APC were purchased from Miltenyi Biotec (Shanghai, China). Anti-Akt (pS473)-PE, anti-STAT3 (pY705)-Alexa fluor 488, anti-p38 MAPK (pT180/pY182)-Alexa fluor 488, anti-SAPK/JNK (pT183/pY185)-PE, anti-β-Catenin (pS45)-PE, anti-p53 (pS37)-Alexa fluor 488, anti-PLK (pT210)-PE, anti-p53-PE, anti-bcl-2-PE, anti- cleaved Caspase-3-FITC and anti- Cyclin D1-FITC were purchased from BD Pharmingen (Shanghai, China). Anti-P21^Waf1/Cip1-PE, anti-cleaved PARP- Alexa fluor 488 and anti-Cyclin B1-Alexa Fluor 488 were purchased from Cell Signaling Technology (Shanghai, China). Rigosertib and decitabine were purchased from Selleck Inc. (Shanghai, China). Both reagents were dissolved in DMSO with a concentration of 10 mM. In a series of experiments, CD34+ cells and cell lines were incubated with 0.5–20 μM rigosertib or 5 μM decitabine in the maintenance medium.

Xu F., He Q., Li X., Chang C.K., Wu L.Y., Zhang Z., Liu L., Shi W.H., Zhu Y., Zhao Y.S., Gu S.C., Fei C.M., Guo J., Wu D, & Zhou L. (2014). Rigosertib as a selective anti-tumor agent can ameliorate multiple dysregulated signaling transduction pathways in high-grade myelodysplastic syndrome. Scientific Reports, 4, 7310.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry of T-cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Purified CD8⁺ T cells, splenocytes and thymocytes were first stained for surface antigens and then treated with Foxp3 staining buffer set according to the manufacturer's directions (eBioscience). Anti-Eomes AlexaFluor 647 or eFluor 660 (Dan11mag, 1/75), anti-T-bet PE (eBio4B10, 1/100) and anti-CD49d FITC or PE (R1-2, 1/50) antibodies were purchased from eBioscience. Anti-CD8 PercP (53–6.7, 1/50), anti-CXCR3 APC (CXCR3-173, 1/50), anti-CD4 Pe-Cy7 (RM4-5, 1/100), anti-CD62L PE (1/100) or V450 (1/50) (MEL-14), anti-Bcl2 PE (3F11, 1/25), anti-Ki67 FITC (B56, 1/25), anti-CD44 FITC or V450 (IM7, 1/50), anti-CD127 Pe-Cy7 (SB/199, 1/50), anti-CD122 FITC (TM-BETA1, 1/50), anti-NK1.1 FITC (PK136, 1/50), anti-CD90.2 Pe-Cy7 (53-2.1, 1/100) and anti-IFNγ APC or PB or PE (XMG1.21/50) were purchased from BD biosciences. Anti-CD3 Pe-Cy7 (2C11, 1/100) was purchased from Biolegend.
In some experiments, brefeldin A (5 μg ml⁻¹, Sigma) was added in samples for 3 h at 37 °C before intracytoplasmic staining. Blood samples were directly stained for surface antigens and then treated with FACS lysing buffer (BD biosciences) as described in the product data sheet. All samples were fixed with 1% paraformaldehyde in PBS prior to their processing using a Cyan flow cytometer (Dako Cytomation).

Martinet V., Tonon S., Torres D., Azouz A., Nguyen M., Kohler A., Flamand V., Mao C.A., Klein W.H., Leo O, & Goriely S. (2015). Type I interferons regulate eomesodermin expression and the development of unconventional memory CD8+ T cells. Nature Communications, 6, 7089.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The fluorochrome labeled monoclonal antibodies (mAbs) anti-CD2-FITC, anti-CD3-Alexa 700, anti-CD3-PerCP, anti-CD4-V450, anti-CD4-PE, anti-CD8-APC Fluor 780, anti-CD8-PacBlue, anti-CD16-FITC, anti-CD20 PECy7, anti-CD19-V450, anti-CD20-APCCy7, anti-CD25-PEcy7, anti- CD28-PE, anti-CD28-FITC, anti-CD38-PE, anti-CD39-FITC, anti-CD45-PerCP, anti-CD56-APC, anti-CD57-FITC, anti-CD197-PECy7, anti-HLA-DR, anti-IgM, anti-IgD, anti-Ki67-FITC, anti-Bcl-2-PE, anti-TNF-α-PE, anti-TNF-α-APC, IFN-γ-PcpCy5.5, and IL-2-PECy7 were purchased from BD Biosciences (Franklin Lakes, NJ). Anti-CD45RA-QDOT655 and anti-CD69-FITC mAb were obtained from Invitrogen (Carlsbad, CA). Anti-CD8-Alexa780, anti-CD27-Alexa Fluor 700, and anti-CD38-eFluor 650NC were obtained from E-bioscience (San Diego, CA). Anti-CD24-PEcy7 and anti-CD279-PE (PD-1) were purchased from Biolegend (San Diego, CA).
Human EBV protein and CMV peptide pool of pp65 sequence consisting of 138 peptides (15 mers with 11 amino acid overlaps) was purchased from JPT Peptide Technologies (Berlin, Germany).
All patients were DSA-free with a calculated panel reactive antibody (PRA) ≤20% at enrollment. Patient samples were assessed for donor-specific alloantibody post-transplantation as described previously ^{(18 (link))}.

Xu H., Samy K.P., Guasch A., Mead S.I., Ghali A., Mehta A., Stempora L, & Kirk A.D. (2015). Post Depletional Lymphocyte Reconstitution During Belatacept and Rapamycin Treatment in Kidney Transplant Recipients. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 16(2), 550-564.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eight-color flow cytometry method was performed using the following monoclonal antibodies: anti-CD4APC Cy7 (BioLegend); anti-CD8BV650 (BD Biociences); anti-CD3Alexa Fluor 700 (eBiocience); anti-CD95FITC (Southern Biotech); anti-Bcl-2PE (BD Biociences), anti-CD14- BV 500 (eBiocience), CD16 PE Cy7 (BioLegend). Far Red Dead cell stain was applied to exclude dead cells (Invitrogen). A matching isotype control for each antibody was included in all experiments.

Torrentes-Carvalho A., Sánchez-Arcila J.C., Azamor T., Barbosa L.S., Hottz E.D., Gandini M., Bozza F.A., da Cunha R.V., de Oliveira Pinto L.M., Damasco P.V, & de Azeredo E.L. (2020). Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease. Scientific Reports, 10, 6351.

+ Open protocol

+ Expand

PBMC Activation and Cytokine Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 1 × 10⁶ PBMCs were stained with the following anti-human mAbs (BD Pharmingen, San Diego, CA): CD3-Pacific Blue (558117), CD56-APC (555518) and CD127-PE (557938) for surface staining. Nonspecific binding was prevented by a short incubation with fetal bovine serum before the addition of specific antibodies. For intracellular cytokine staining, PBMCs were incubated at 37°C for 72h in a 24-well plate (1 X 10⁶ cells/well) with or without IL-7 (50 ng/ml) and followed by a mixture of PMA (20 ng/ml), Ionomycine (1 μg/ml) and GolgiPlug (1μg/ml) for another 5h at 37°C. After harvesting, cells were stained for surface markers CD3-Pacific Blue (558117) and CD56-APC (555518) followed by fixation and permeabilization with the Cytofix/Cytoperm kit (BD). They were then respectively stained with anti-IFN-γ-PE (BD, 340452) or anti-Bcl-2-PE (BD, 340651) as designed. After staining, all samples were acquired on a FACSAria^™ flow cytometer and analyzed with FACSDiva software 6.1.3 or FCS Express 4 software (BD, Mountain View, CA).

Su N., Shi S.X., Zhu X., Borazanci A., Shi F.D, & Gan Y. (2014). Interleukin-7 expression and its effect on natural killer cells in patients with multiple sclerosis. Journal of neuroimmunology, 276(0), 180-186.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!