Anti mouse igg h l alexa fluor 488 secondary antibody

After fixation with 4% paraformaldehyde, THP-1 cells were blocked in 0.2% bovine serum albumin at room temperature for 1 h, and then incubated with primary antibody of Arg1(1:100, Abcam, ab239731) overnight at 4 °C. After being washed, the cells were incubated with anti-mouse IgG H&L (Alexa Fluor 488) secondary antibody (1: 500, Abcam, ab150113) at room temperature for 2 h, followed by counter-staining of the nuclei with 4,6-diamidino-2-phenylindole (DAPI) (Solarbio^®, USA). Finally, samples were visualized and photographed by a blinded observer with an Olympus IX-71 fluorescence microscope (Tokyo, Japan). Quantification of Arg1⁺ cells in at least five random fields of views was undertaken using ImageJ software (National Institutes of Health, Bethesda, MA, USA).