Log In Sign up
The largest database of trusted experimental protocols

Autodelfia hgh

Manufactured by PerkinElmer
Visit Supplier
Sourced in United Kingdom

The AutoDELFIA® hGH is a laboratory instrument designed for the measurement of human growth hormone (hGH) levels. It utilizes a time-resolved fluoroimmunoassay (TR-FIA) technology to quantify hGH concentrations in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

7600 biochemical analyzer, by Hitachi (4 mentions) Advia centaur xp, by Siemens (2 mentions) Hlc 723g8 hplc analyzer, by Tosoh (1 mentions) Nitrate nitrite colorimetric assay kit, by Cayman Chemical (1 mentions)

8 protocols using autodelfia hgh

1

Endocrine Biomarker Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
GH was measured by a two-site chemiluminescent immunometric assay (AutoDELFIA® hGH, PerkinElmer Life and Analytical Sciences, Wallac Oy), intra-assay CV: 5.3–6.5%, interassay CV: 5.7–6.2%, and sensitivity: up to 0.01 μg/l (0.026 mU/l).
IGF-1 was measured with the IMMULITE 2000 solid-phase, enzyme-labeled chemiluminescent immunometric assay (Siemens Healthcare Diagnostic Products Limited, UK); normal age-appropriate ranges are as follows: 1–6 years: 49–327 μg/l; 7–11 years: 57–551 μg/l; 12–13 years: 143–850 μg/l; 14–16 years: 220–996 μg/l; 17–18 years: 163–731 μg/l; 19–20 years: 127–483 μg/l; 21–35 years: 115–358 μg/l; 36–50 years: 94–284 μg/l; >50 years: 55–238 μg/l; intra-assay CV: 2.3–3.5%; interassay CV: 7.0–7.1%; and sensitivity: 20 μg/l. IGF-1 index = IGF − 1/upper limit of normal range (ULN) [24 (link)].
Insulin was measured by chemiluminescence immunoassay (ADVIA Centaur XP, Siemens, USA). BG was measured by a Hitachi 7600 Biochemical Analyzer (Tokyo, Japan). HbA1c was detected with high-performance liquid chromatography (Tosoh HLC-723 G8 HPLC Analyzer, Japan).
Shen M., Wang M., He W., He M., Qiao N., Ma Z., Ye Z., Zhang Q., Zhang Y., Yang Y., Cai Y., ABuDuoReYiMu Y., Lu Y., Lu B., Shou X., Wang Y., Ye H., Li Y., Li S., Zhao Y., Cao X, & Zhang Z. (2018). Impact of Long-Acting Somatostatin Analogues on Glucose Metabolism in Acromegaly: A Hospital-Based Study. International Journal of Endocrinology, 2018, 3015854.
+ Open protocol
+ Expand
2

Hormonal and Vascular Evaluation in Acromegaly

Check if the same lab product or an alternative is used in the 5 most similar protocols
Random GH, GH nadir (GHn) after 75 g OGTT, and IGF-1 levels were measured. GH was measured by a 2-site chemiluminescent immunometric assay (AutoDELFIA® hGH, PerkinElmer Life and Analytical Sciences, Wallac Oy, Finland). IGF-1 was measured using an Immulite 2000 solid-phase, enzyme-labeled chemiluminescent immunometric assay (Siemens Healthcare Diagnostic Products Limited, UK). The IGF-1 index was calculated as the ratio of the IGF-1 level/the upper limit of age-adjusted reference ranges (14 (link)). The criteria of endocrine remission was defined as the reduction of IGF-1 levels to age-adjusted normal ranges, random GH levels to<1.0 μg/L and GHn levels <0.4 μg/L after OGTT (15 (link)). Other adenohypophyseal hormones including thyroid-stimulating hormone (TSH), free thyroxine (FT4), morning free cortisol, follicle-stimulating hormone (FSH), luteinizing hormone (LH), total testosterone, and prolactin (PRL) were measured. The measurement of serum NO levels was performed to assess endothelium function using the Nitrate/Nitrite Colorimetric Assay kit (Cayman, Ann Arbor, MI).
Chen Z., Shao X., He M., Shen M., Gong W., Wang M., Zhang Y., Liu W., Ma Z., Ye Z., Lu Y., Yang N., Chen S., Hu L., Li Y., Wang Y., Zhao Y, & Zhang Z. (2021). Erectile Dysfunction Is Associated With Excessive Growth Hormone Levels in Male Patients With Acromegaly. Frontiers in Endocrinology, 12, 633904.
+ Open protocol
+ Expand
3

Biochemical Measures in Pediatric Growth Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols
BG was measured by Hitachi 7600 Biochemical Analyzer (Tokyo, Japan). Insulin was measured by the chemiluminescence immunoassay (ADVIA Centaur XP, Siemens, USA). HbA1c was detected with high-performance liquid chromatography (Tosoh HLC-723 G8 HPLC Analyzer, Japan).
GH was measured by a two-site chemiluminescent immunometric assay AutoDELFIA® hGH (PerkinElmer Life and Analytical Sciences, Wallac Oy), intra-assay CV: 5.3–6.5%, interassay CV: 5.7–6.2%, and sensitivity: up to 0.01 μg/l (0.026 mU/l).
IGF-1 was measured with IMMULITE 2000 solid-phase, enzyme-labeled chemiluminescent immunometric assay (Siemens Healthcare Diagnostic Products Limited, UK), with an age-specific normal range (1–6 years: 49–327 μg/l; 7–11 years: 57–551 μg/l; 12-13 years: 143–850 μg/l; 14–16 years: 220–996 μg/l; 17-18 years: 163–731 μg/l; 19-20 years: 127–483 μg/l; 21–35 years: 115–358 μg/l; 36–50 years: 94–284 μg/l; >50 years: 55–238 μg/l; intra-assay CV: 2.3–3.5%; interassay CV: 7.0–7.1%; sensitivity: 20 μg/l).
Sun Q., Li X., Chen P., Chen L, & Zhao X. (2021). The Beta-Cell Function and Glucose Profile of Newly Diagnosed Acromegalic Patients with Normal Glucose Tolerance. International Journal of Endocrinology, 2021, 3666692.
+ Open protocol
+ Expand
4

Measurement of IGF-1 and GH Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
IGF-1 was measured with Immulite 2000 solid-phase, which is an enzyme-labeled chemiluminescent immunometric assay (Siemens Healthcare Diagnostic Products Limited, UK). The normal ranges were age-dependent (1–6 years: 49–327 μg/L; 7–11 years: 57–551 μg/L; 12-13 years: 143–850 μg/L; 14–16 years: 220–996 μg/L; 17-18 years: 163–731 μg/L; 19-20 years: 127–483 μg/L; 21–35 years: 115-358 μg/L; 36–50 years: 94–284 μg/L; and >50 years: 55–238 μg/L) [18 (link)]. The IGF-1 index, a parameter that describes the level of the IGF-1 based on age (defined as the ratio of IGF-1 value to the maximum of reference ranges), was calculated for all patients. GH was measured by a two-site chemiluminescent immunometric assay using AutoDELFIA® hGH (PerkinElmer Life and Analytical Sciences, Wallac Oy).
Liu W., Xie L., He M., Shen M., Zhu J., Yang Y., Wang M., Hu J., Ye H., Li Y., Zhao Y, & Zhang Z. (2017). Expression of Somatostatin Receptor 2 in Somatotropinoma Correlated with the Short-Term Efficacy of Somatostatin Analogues. International Journal of Endocrinology, 2017, 9606985.
+ Open protocol
+ Expand
5

Growth Hormone and IGF-1 Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols
GH was measured by a two-site chemiluminescent immunometric assay (AutoDELFIA hGH, PerkinElmer Life and Analytical Sciences, Boston, USA), and the intra-assay coefcient of variation (CV) was 5.3–6.5%, inter-assay CV was 5.7–6.2%, and sensitivity was up to 0.01 μg/L (0.026 mU/L). IGF-1 was measured with the Immulite 2000 solid-phase, enzyme-labeled chemiluminescent immunometric assay (Siemens Healthcare Diagnostic Products Limited, Marburg, Germany). The intra-assay CV was 2.3–3.5%; inter-assay CV, 7.0–7.1%; and sensitivity, 20 μg/L.
Zhang Y., Wang M., Ji C., Chen Z., Yang H., Wang L., Yu Y., Qiao N., Ma Z., Ye Z., Shao X., Liu W., Wang Y., Gong W., Melnikov V., Hu L., Lee E.J., Ye H., Wang Y., Li Y., He M., Zhao Y, & Zhang Z. (2021). Treatment of acromegaly by rosiglitazone via upregulating 15-PGDH in both pituitary adenoma and liver. iScience, 24(9), 102983.
+ Open protocol
+ Expand
6

Measurement of GH and IGF-1 Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
GH and IGF-1 were measured as described previously (78 (link)). Briefly, GH was measured by a two-site chemiluminescent immunometric assay (AutoDELFIA hGH, PerkinElmer Life and Analytical Sciences, Wallac Oy), with intraassay coefficient of variation (CV) of 5.3 to 6.5%, interassay CV of 5.7 to 6.2%, and sensitivity up to 0.01 μg/L (0.026 mU/L).
IGF-1 was measured with the Immulite 2000 solid-phase, enzyme-labeled chemiluminescent immunometric assay (Siemens Healthcare Diagnostic Products Limited, UK); normal age-appropriate ranges are as follows:1 to 6 years, 49 to 327 μg/L; 7 to 11 years, 57 to 551 μg/L; 12 to 13 years, 143 to 850 μg/L; 14 to 16 years: 220 to 996 μg/L; 17 to 18 years, 163 to 731 μg/L; 19 to 20 years, 127 to 483 μg/L; 21 to 35 years, 115 to 358 μg/L; 36 to 50 years, 94 to 284 μg/L; >50 years, 55 to 238 μg/L; intraassay CV, 2.3 to 3.5%; interassay CV, 7.0 to 7.1%; sensitivity, 20 μg/L. IGF-1 index = IGF-1/upper limit of normal range (ULN) (79 (link)).
Plasma glucose was measured by HITACHI 7600 Biochemical Analyzer (Tokyo, Japan).
Lin B., Wang M., Gao R., Ye Z., Yu Y., He W., Qiao N., Ma Z., Ji C., Shi C., Zhou X., Wang Y., Zeng F., Zhang L., Gong W., Cao Z., Zhou P., Melnikov V., Ye H., Li Y., Zhang Z., He M., Qin H, & Zhang Y. (2022). Characteristics of Gut Microbiota in Patients with GH-Secreting Pituitary Adenoma. Microbiology Spectrum, 10(1), e00425-21.
+ Open protocol
+ Expand
7

Measurement of GH and IGF-1 Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
GH and IGF-1 were measured as described previously (78 (link)). Briefly, GH was measured by a two-site chemiluminescent immunometric assay (AutoDELFIA hGH, PerkinElmer Life and Analytical Sciences, Wallac Oy), with intraassay coefficient of variation (CV) of 5.3 to 6.5%, interassay CV of 5.7 to 6.2%, and sensitivity up to 0.01 μg/L (0.026 mU/L).
IGF-1 was measured with the Immulite 2000 solid-phase, enzyme-labeled chemiluminescent immunometric assay (Siemens Healthcare Diagnostic Products Limited, UK); normal age-appropriate ranges are as follows:1 to 6 years, 49 to 327 μg/L; 7 to 11 years, 57 to 551 μg/L; 12 to 13 years, 143 to 850 μg/L; 14 to 16 years: 220 to 996 μg/L; 17 to 18 years, 163 to 731 μg/L; 19 to 20 years, 127 to 483 μg/L; 21 to 35 years, 115 to 358 μg/L; 36 to 50 years, 94 to 284 μg/L; >50 years, 55 to 238 μg/L; intraassay CV, 2.3 to 3.5%; interassay CV, 7.0 to 7.1%; sensitivity, 20 μg/L. IGF-1 index = IGF-1/upper limit of normal range (ULN) (79 (link)).
Plasma glucose was measured by HITACHI 7600 Biochemical Analyzer (Tokyo, Japan).
Lin B., Wang M., Gao R., Ye Z., Yu Y., He W., Qiao N., Ma Z., Ji C., Shi C., Zhou X., Wang Y., Zeng F., Zhang L., Gong W., Cao Z., Zhou P., Melnikov V., Ye H., Li Y., Zhang Z., He M., Qin H, & Zhang Y. (2022). Characteristics of Gut Microbiota in Patients with GH-Secreting Pituitary Adenoma. Microbiology Spectrum, 10(1), e00425-21.
+ Open protocol
+ Expand
8

Growth Hormone and IGF-1 Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols
GH levels were analyzed using a two-site chemiluminescence immunoassay (AutoDELFIA ® hGH, PerkinElmer Life and Analytical Sciences, Wallac Oy) with an intraand inter-assay variance of 5.3-6.5% and 5.7-6.2%, respectively, and a sensitivity of 0.01 μg/L. IGF-1 levels were analyzed using Immulite 2000 solid phase, enzymelabeled chemiluminescence immunoassay analysis (Siemens Medical Diagnostics Ltd., United Kingdom), with an intra-and inter-assay variance of 2.3-3.5% and 7.0-7.1%, respectively, and a sensitivity of 20 μg/L [21] . Normal age-appropriate ranges of IGF-1 for adults are:19-20 years, 127-483 μg/L; 21-35 years, 115-358 μg/L; 36-50 years, 94-284 μg/L; >50 years, 55-238 μg/L [22] .
Lin B., He W., Chen Z., Shen M., Shou X., Chen L., Ma Z, & Wang Y. (2023). Self-reported symptoms in patients with acromegaly: a 6-month follow-up in a single neurosurgical center. Endocrine journal, 70(1).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!