Lats2fl

C57BL/6, CD45.1⁺, OT-I, OT-II, Batf3^−/−, B2m^−/−, Il12a^−/−, Mtor^fl, Map3k14^−/−, Lats1^fl, Lats2^fl, and CD11c-Cre mice were purchased from The Jackson Laboratory. Stk4^fl and Stk3^fl mice were kindly provided by Randy Johnson ^{21 (link)}, and Yap^fl and Taz^fl mice were kindly provided by Eric Olson^{22 (link)}. The mice have been backcrossed to the C57BL/6 background and were used at 8-12 weeks old. All of the genetically modified mice were viable and developed normally. For mixed bone marrow (BM) chimera generation, BM cells from WT or Mst1/2^ΔDC CD45.2.2⁺ mice were mixed with cells from CD45.1.2⁺ mice at a 1:1 ratio and transferred into lethally irradiated (11 Gy) CD45.1.1⁺ mice, followed by reconstitution for 6-8 weeks, as described previously^{23 (link)}. In certain experiments, BM cells from WT or Mst1/2^ΔDC CD45.2.2⁺ mice were transferred into lethally irradiated (11 Gy) CD45.1.1⁺ mice. For chimeras used in Fig. 2c, BM cells from WT or Mst1/2^ΔDC CD45.2.2⁺ mice were mixed with BM cells from Batf3^−/− mice at a 1:1 ratio and transferred into lethally irradiated (11 Gy) CD45.1.1⁺ mice. All mice were kept in a specific pathogen-free facility in the Animal Resource Center at St. Jude Children’s Research Hospital. Animal protocols were approved by the Institutional Animal Care and Use Committee of St. Jude Children’s Research Hospital.