Control sirna sc 36869

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Control siRNA (sc-36869) is a non-targeting siRNA sequence that serves as a negative control for RNA interference (RNAi) experiments. It is designed to have no known complementary targets in mammalian cells.

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Lab products found in correlation

Lipofectamine 2000 reagent, by Thermo Fisher Scientific (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Sirnas, by Santa Cruz Biotechnology (1 mentions) Sc 41109, by Santa Cruz Biotechnology (1 mentions) Sc 41113, by Santa Cruz Biotechnology (1 mentions) Lipofectamine 2000 kit, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Ab178945, by Abcam (1 mentions) Sc 67143, by Santa Cruz Biotechnology (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Mimic control, by GenePharma (1 mentions) Inhibitor control, by GenePharma (1 mentions) Mir 374 5p inhibitor, by Thermo Fisher Scientific (1 mentions) Il 10 sirna, by GenePharma (1 mentions) Control sirna, by GenePharma (1 mentions) Pcdna3.1 vector, by Genechem (1 mentions)

5 protocols using control sirna sc 36869

Knockdown of Sirt1 and Trim72 in Cells

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Sirt1 siRNA (m) (sc-20987), Trim72 siRNA (m) (sc-154670), and control siRNA (sc-36869) were bought from Santa Cruz (Santa Cruz, CA, USA). Cells were transfected using LipofectamineTM 3000 (Thermo Fisher, USA) to knockdown the genes of Sirt1 and Trim72. After treating the cells with drugs, they were analyzed using Western blotting, immunofluorescence, β-galactosidase staining, ROS staining, and fluo-3AM staining.

Xie W., Deng L., Qian R., Huang X., Liu W, & Tang S. (2024). Curculigoside Attenuates Endoplasmic Reticulum Stress-Induced Epithelial Cell and Fibroblast Senescence by Regulating the SIRT1-P300 Signaling Pathway. Antioxidants, 13(4), 420.

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siRNA Knockdown of Wnt Genes in MSCs

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siRNAs specific for mouse Wnt3a (sc-41109), Wnt5a (sc-41113) and control siRNA (sc-36869) with scrambled sequences were purchased from Santa Cruz. In each transfection, 0.1 to 0.2 μM siRNAs were introduced into MSCs using the Lipofectamine 2000 kit (Invitrogen, USA). The efficiency of transduction was determined by detecting the rate of FITC-positive cells and the mRNA expression of specific genes.

He X., Wang H., Jin T., Xu Y., Mei L, & Yang J. (2016). TLR4 Activation Promotes Bone Marrow MSC Proliferation and Osteogenic Differentiation via Wnt3a and Wnt5a Signaling. PLoS ONE, 11(3), e0149876.

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Silencing YY1 Transcription Factor in Ramos Cells

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Ramos cells were transfected with siRNA for YY1 and corresponding irrelevant controls by liposomes. Cells were treated with 30 nM siRNA YY1 (YY1 siRNA (h) SC-36863) or control siRNA (SC-36869) (Santa Cruz Biotechnology, Inc.) and transfected using Lipofectamine 2000 (Invitrogen, Thermo Fisher Scientific, USA) following the manufacturer's instructions. The transfected cells were incubated at 37° C and 5% CO2 for 48 hrs. Subsequently, a western blot was performed to determine the expression of YY1 and KLF4 in cells treated with siRNA.

Morales-Martinez M., Valencia-Hipolito A., Vega G.G., Neri N., Nambo M.J., Alvarado I., Cuadra I., Duran-Padilla M.A., Martinez-Maza O., Huerta-Yepez S, & Vega M.I. (2019). Regulation of Krüppel-Like Factor 4 (KLF4) expression through the transcription factor Yin-Yang 1 (YY1) in non-Hodgkin B-cell lymphoma. Oncotarget, 10(22), 2173-2188.

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Knockdown of iNOS and 15-LOX-2 in EOC 20 Cells

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A pool of three target-specific 19-25 nt siRNAs designed to knock down iNOS and 15-LOX-2 expression in the EOC 20 cells was utilized. Control siRNA (sc-36869) and iNOS- (NOS2 siRNA (m): sc-36092) and 15-LOX-2-siRNA (15-LOX-2 siRNA (m): sc-45627) were designed and synthesized by Santa Cruz Biotechnology, Inc. Transfection of the control-siRNA and the target-siRNA was performed using Lipofectamine RNAi Max (Invitrogen) following the manufacturer’s instructions. EOC 20 cells were seeded onto 6 well-plates at 1~2x10⁵ cells /1 ml /well for incubations up 7 h, then the transfection mixture was removed and cells were switched to DMEM supplemented with 10% FBS for 24 h. Knock down efficiency was assessed by western blot analysis using the following antibodies: anti-iNOS (ab178945, Abcam, 1:500), anti-15-LOX-2 (sc-67143, Santa Cruz, 1:500), horseradish peroxidase (HRP)-conjugated anti-rabbit IgG H&L (Sigma, A0545, 1:2000) secondary antibody, and anti-β-actin-peroxidase antibody (ThermoFisher, Cat # A3854,1:5000). To assess the effect of KD on ferroptosis cells were plated in 24-well plate (15000 - 20000 cells per well), incubated with RSL3 and cell death was assessed by measuring released LDH activity.
Sequences of siRNA used for transfection of EOC 20:

Kapralov A.A., Yang Q., Dar H.H., Tyurina Y.Y., Anthonymuthu T.S., Kim R., St. Croix C.M., Mikulska-Ruminska K., Liu B., Shrivastava I.H., Tyurin V.A., Ting H.C., Wu Y.L., Gao Y., Shurin G.V., Artyukhova M.A., Ponomareva L.A., Timashev P.S., Domingues R.M., Stoyanovsky D.A., Greenberger J.S., Mallampalli R.K., Bahar I., Gabrilovich D.I., Bayir H, & Kagan V.E. (2020). Redox Lipid Reprogramming Commands Susceptibility of Macrophages and Microglia to Ferroptotic Death.. Nature chemical biology, 16(3), 278-290.

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In Vitro IDD Model Establishment

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Human NP cells were purchased from American Type Culture Collection (ATCC) and cultured in Dulbecco's Modified Eagle Medium (DMEM)/F12 medium (with 10% fetal bovine serum and 1% penicillin‐streptomycin) at 37°C using a 5% CO₂ incubator. Cells were cultured in monolayer and the 2−3 generations of cells were used in the following experiments. Experiments were performed for three times.
To construct an in vitro model of IDD, we treated NP cells with 10 ng/ml of LPS for 24 h.
The lncRNA MAGI2‐AS3 sequence was synthesized based on the lncRNA MAGI2‐AS3 sequence and then subcloned into the pcDNA3.1 vector (MAGI2‐AS3‐plasmid; Shanghai GeneChem Co., Ltd.). The empty pcDNA3.1 vector was used as a control (control‐plasmid). For IL‐10 knockdown, the control‐siRNA (sc‐36869) and IL‐10‐siRNA (sc‐39634) were purchased from Santa Cruz Biotechnology. NP cells were transfected with control‐plasmid, MAGI2‐AS3‐plasmid, control‐siRNA, IL‐10‐siRNA, mimic control (Shanghai GenePharma), miR‐374‐5p mimic (Shanghai GenePharma), inhibitor control (Shanghai GenePharma), miR‐374‐5p inhibitor (Shanghai GenePharma), MAGI2‐AS3‐plasmid+mimic control, MAGI2‐AS3‐plasmid + miR‐374‐5p mimic, miR‐374‐5p inhibitor+control‐siRNA, or miR‐374‐5p inhibitor + IL‐10‐siRNA using Lipofectamine 2000 reagent (Thermo Fisher Scientific, Inc.) at 37°C for 24 h as per the manufacturer's protocol.

Yu J, & Li C. (2023). Role of lncRNA MAGI2‐AS3 in lipopolysaccharide‐induced nucleus pulposus cells injury by regulating miR‐374b‐5p/interleukin‐10 axis. Immunity, Inflammation and Disease, 11(4), e772.

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