Cst 29

Synthetic human Aβ1-42 (catalog number AS-24224), Aβ1-40 (catalog number AS-24236), SST14 (catalog number AS-24277), [Arg8]-Vasopressin (catalog number AS-24289), Bombesin (catalog number AS-20665), Oxytocin (catalog number AS-24275), SST28 (catalog number AS-22902) and amylin1-37 (catalog number AS-60804) were purchased from Anaspec, Inc. (Fremont, CA, USA). The alternative synthetic human SST14 peptide (catalog number H-1490) sourced from a different vendor (see Figure 4B), CST17 (catalog number H-5536) and CST29 (catalog number H-6458) were obtained from Bachem Americas, Inc. (Torrance, CA, USA). The biotinylated Aβ peptides, including biotin-Aβ1-42, Aβ1-42-biotin, Aβ17-42-biotin, biotin-SST14 and truncated or mutant SST peptides were synthesized by LifeTein LLC (Hillsborough, NJ, USA). The Edans-SST donor and Aβ1-42-TMR acceptor peptides were in-house synthesized as described previously (Bateman and Chakrabartty, 2011 (link)).

BBB integrity was analysed by evaluating Evans blue extravasation. For this, 1-year-old Cort^+/+ and Cort^−/− mice were injected intraperitoneally with LPS from E.coli 055:B5 (6 mg/kg, Sigma) inducing mild neuroinflammation [20 (link)]. Immediately after LPS injection, 1 nmol of CST-29 (resuspended in PBS, Bachem) was intraperitoneally injected. 5 h after LPS and LPS + CST injection, Evans Blue (EB, 2% in saline, 4 ml/kg) was injected through the tail vein. Control animals were injected with vehicle (PBS). After 1 h, mice were sacrificed by intraperitoneal injection of pentobarbital and intracardially perfused with PBS. EB quantification was performed as indicated in Additional file 1. Data were represented as μg of dye per mg of brain tissue.

BBB integrity was analysed by evaluating Evans blue extravasation. For this, 1-year-old Cort^+/+ and Cort^−/− mice were injected intraperitoneally with LPS from E.coli 055:B5 (6 mg/kg, Sigma) inducing mild neuroinflammation [20 (link)]. Immediately after LPS injection, 1 nmol of CST-29 (resuspended in PBS, Bachem) was intraperitoneally injected. 5 h after LPS and LPS + CST injection, Evans Blue (EB, 2% in saline, 4 ml/kg) was injected through the tail vein. Control animals were injected with vehicle (PBS). After 1 h, mice were sacrificed by intraperitoneal injection of pentobarbital and intracardially perfused with PBS. EB quantification was performed as indicated in Additional file 1. Data were represented as μg of dye per mg of brain tissue.