Rabbit anti human nlrp3

Antibodies were from the following sources: anti-mouse α-smooth muscle actin (Clone 1A4; Sigma-Aldrich), mouse anti-human β-tubulin (Sigma), rabbit anti-human NLRP3 (Sigma), mouse anti-human NLRP3 (Cryo-2, Adipogen), rabbit anti-human Smad 2/3 (Santa Cruz Biotechnology), mouse anti-human KIM-1/TIM-1 (R&D Systems), mouse anti-human E-cadherin (clone 36; BD Biosciences), rabbit anti-human Zo1 (Invitrogen), rabbit anti-human calreticulin (Abcam), rabbit anti-human cytochrome C (Abcam), rabbit anti-human GM130 (Abcam), and rabbit anti-human podocin (Sigma). All primary antibodies were used at a dilution of 1:1000 for immunoblotting and 1:200 for immunofluorescence respectively unless otherwise specified. For mitochondrial labelling, 200 nM of Mitotracker Red CMXRos (Invitrogen) was added to the culture medium and cells incubated for 15 min prior to fixation of cells.

Mouse anti-human IL-1β, 1∶250 (R&D Systems); rabbit anti-human NLRP3, 1∶250 (Sigma); rabbit anti-human ASC 1∶200 (Santa Cruz); goat anti-Annexin A2, 1∶200 (Santa Cruz); mouse anti-pSTAT3, 1∶250 (Cell Signaling), rabbit anti-STAT3, 1∶1,000 (Cell Signaling); mouse anti-β-actin, 1∶500 (Cell Signaling); anti-rabbit IgG conjugated with HRP, 1∶500 (ThermoScientific/Pierce); anti-mouse IgG conjugated with HRP, 1∶500 (ThermoScientific/Pierce); anti-goat IgG conjugated with HRP, 1∶5,000 (Rockland Immunochemicals); ImmPRESS polymer detection reagent (HRP conjugated anti-mouse IgG) (Vector Laboratories).