ALA (463-40-1) (0.914gm/ml) was purchased from TCI Chemicals (India) Pvt. Ltd. RPMI 1640 (Gibco, USA); fetal bovine serum (FBS) (Gibco-10270); trypsin (Gibco, USA); eagle balanced salt solution (EBSS)(Gibco, 2018-11); hank’s balanced salt solution (HBSS) (Himedia, TL1190); EtBr (Himedia, MB071); AO(Himedia, MB116); JC-1 assay kit (Thermo Scientific, M34152); PI (SC-3541); tamoxifen citrate (TMX) (Tammodex 20, Biochem Pharmaceuticals India); penicillin- streptomycin (Thermo Scientific, 15410-163); gentamycin (Thermo Scientific, 15710-049);3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) (Himedia, TC191); RNase (SRL, 58895); dimethyl sulfoxide (DMSO) (Merck, 1.16743.0521); DMBA (Sigma Aldrich, 57-97-6); ponceau S (Himedia, ML045); sodium cacodylate (Sigma Aldrich, C0250); collagenase type 4 (Himedia, TC-214); hyaluronidase (Himedia, TC331); hematoxylin (Himedia, S058); eosin (Himedia, S007); RIPA lysis buffer (Amresco, N653); protein assay kit (Amresco, M173); bovine serum albumin (BSA) (Genetix, PG-2330); transfer buffer (Genetix, GX-9411AR), trizol reagent (Sigma-T9424), cDNA synthesis kit (Genetix-K1612). Caspase 3 (SC-4263) and caspase 8 (SC-4267) assay kits were procured from Santacruz Biotechnology Inc., California, Delaware. All others chemicals were of molecular biology grade and purchased from Genetix Biotech Asia Pvt. Ltd, New Delhi.
Cdna synthesis kit
Manufactured by Genetix
Sourced in India, United States
The cDNA Synthesis Kit is a laboratory tool used for the conversion of RNA molecules into complementary DNA (cDNA) strands. It provides the necessary reagents and protocols for efficient and reliable cDNA synthesis, which is a fundamental step in various molecular biology applications, such as gene expression analysis and reverse transcription-PCR.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Gentamycin, by Thermo Fisher Scientific (1 mentions)
Collagenase type 4, by HiMedia (1 mentions)
Transfer buffer, by Genetix (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Ponceau s, by HiMedia (1 mentions)
Hanks balanced salt solution (hbss), by HiMedia (1 mentions)
Jc 1 assay kit, by Thermo Fisher Scientific (1 mentions)
Protein assay kit, by Avantor (1 mentions)
Ripa lysis buffer, by Avantor (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Merck Group (1 mentions)
Sodium cacodylate, by Merck Group (1 mentions)
Hematoxylin, by HiMedia (1 mentions)
Crystal violet, by HiMedia (1 mentions)
Glabridin, by Merck Group (1 mentions)
Lysostaphin, by Merck Group (1 mentions)
Tryptic soy broth (tsb), by HiMedia (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
2 protocols using cdna synthesis kit
1
Evaluating Cytotoxicity and Apoptosis in Cancer Cells
2
Antibacterial Evaluation of Glabridin
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The drug-sensitive strain MTCC 96 (ATCC9144) of S. aureus was procured from the Microbial Type Culture Collection, CSIR-Institute of Microbial Technology Chandigarh, India. Clinical isolates of S. aureus were obtained from Dr. K. N. Prasad (Laboratory of Clinical Microbiology, SGPGIMS, Lucknow, India) (Gupta et al., 2012 (link)). Standard Mueller-Hinton agar and cation-adjusted Mueller-Hinton broth media (MHA and MHB, Hi-Media, Mumbai, India) were used to culture bacteria. Tryptic soy broth (TSB, Hi-Media, Mumbai, India) medium was used for Biofilm assays. Glabridin, antibiotics, phosphate-buffered saline, DMSO, glucose and lysostaphin was procured from Sigma-Aldrich (St. Louis, MO, United States). Crystal Violet used in this study was used from Hi-Media (Mumbai, India). Trypsin Gold, Mass Spectrometry Grade was obtained from Promega (Madison, WI, United States). Taq DNA polymerase master mix, c-DNA synthesis kit and SYBR Green qPCR super mix was purchased from Genetix (Thermo, United States).
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