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2 3 7 8 tetrachlorodibenzo p dioxin

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2,3,7,8-tetrachlorodibenzo-p-dioxin is a chemical compound used for analytical and research purposes. It is a polychlorinated dibenzo-p-dioxin with the formula C₁₂H₄Cl₄O₂.

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Lab products found in correlation

Verapamil, by Merck Group (1 mentions) Phenobarbital, by Merck Group (1 mentions) Rhodamine 123, by Merck Group (1 mentions) Probenecid, by Merck Group (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Chenodeoxycholic acid, by Merck Group (1 mentions) Rifampicin, by Merck Group (1 mentions) Fumitremorgin c, by Merck Group (1 mentions) Tert butylhydroquinone, by Merck Group (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Trichloroacetic acid, by Merck Group (1 mentions) Hepg2, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Cytiva (1 mentions) H4iie, by American Type Culture Collection (1 mentions) Hepa1c1c7, by American Type Culture Collection (1 mentions) Resorufin ethyl ether, by Merck Group (1 mentions) α naphthoflavone, by Merck Group (1 mentions) 4 hydroxynonenal, by Cayman Chemical (1 mentions) Dmso control, by Merck Group (1 mentions)

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2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) (6 citations)

4 protocols using 2 3 7 8 tetrachlorodibenzo p dioxin

1

Quantification of Drug Transporter Expression

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Rhodamine 123, probenecid, verapamil, fumitremorgin C, rifampicin, phenobarbital, chenodeoxycholic acid (CDCA) and tert-butylhydroquinone (tBHQ) were provided by Sigma-Aldrich (Saint-Quentin Fallavier, France), whereas carboxy-2,7-dichlorofluorescein (CF) diacetate and Hoechst 33342 were from Life Technologies (Saint Aubin, France) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) from Cambridge Isotope Laboratories (Cambridge, UK). [3H(G)] taurocholic acid (sp. act. 5.0 Ci/mmol), [6,7-3H(N)] estrone-3-sulfate (E3S) (sp. act. 54.0 Ci/mmol) and [1-14C] tetra-ethylammonium (TEA) (sp. act. 3.5 mCi/mmol) were supplied by Perkin-Elmer (Boston, MA, USA). Mouse monoclonal antibodies against P-gp (clone C219), MRP2 (clone M2III-6) and MRP3 (ABCC3) (clone M3II-9) were from Alexis Biochemicals (Lausen, Switzerland), whereas rabbit antibody against the p38 mitogen-activated protein kinase (MAPK) was provided by Santa Cruz Biotechnology (Dallas, TX, USA). Mouse monoclonal antibody against MRP4 (ABCC4) and rabbit polyclonal antibody against MRP5 (ABCC5) were purchased from Abcam (Paris, France). All other chemicals and reagents were commercial products of the highest purity available.
Jouan E., Le Vée M., Denizot C., Parmentier Y, & Fardel O. (2016). Drug Transporter Expression and Activity in Human Hepatoma HuH-7 Cells. Pharmaceutics, 9(1), 3.
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2

TCDD and GNF-351 Cytotoxicity Assay

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2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD, 50 μg/mL in DMSO, >99.9%) was purchased from Cambridge Isotope Laboratories (Tewksbury, MA) and GNF-351 was purchased from Santa Cruz Biotechnology (Dallas, TX). Trichloroacetic acid was purchased from Merck (Darmstadt, Germany). Resorufin ethyl ether, 3,3′-methylene-bis(4-hydroxycoumarin), sulforhodamine B and DMEM/F12 powder were purchased from Sigma (St Louis, MO). 100x L-glutamine and penicillin-streptomycin were purchased form Gibco (Dublin, Ireland) while fetal bovine serum was purchased from Cytiva Life Sciences (Marlborough, MA). HepG2, Hepa1c1c7 and H4IIE cell lines were purchased from ATCC (Manassas, VA).
King J., Woolner V.H., Keyzers R.A, & Rosengren R.J. (2022). Characterization of marine-derived halogenated indoles as ligands of the aryl hydrocarbon receptor. Toxicology Reports, 9, 1198-1203.
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3

Assessing AhR Signaling in 4HNE Cytotoxicity

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To determine the role of AhR signaling in maintaining cell viability in light of 4HNE-mediated cytotoxicity, cells were plated in a 96-well format with a density of 150,000 cells per plate. Cells were then treated with desired concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (Cambridge Isotope Laboratories), 2-(2-aminophenyl) (Sigma-Aldrich), palmitoleic acid (Cayman Chemical Company) or control DMSO (Sigma-Aldrich). In determining viability dependence on AhR, cells were co-treated with AhR ligands and desired concentrations of α-naphthoflavone (Sigma-Aldrich). Molecule treatments lasted for 48 hours and were and co-treatment with 4-hydroxynonenal (Cayman Chemical Company) for 24 hours. At which point, cell viability was assessed using the CellTiter-Glo luminescent cell viability kit (Promega. Inc) and a Fluoroskan Ascent FL luminometer. Cell viability was also accessed using Calcein-AM kit (Life. Inc.) and a Spectramax fluorescence plate reader.
Gutierrez M.A., Davis S.S., Rosko A., Nguyen S.M., Mitchell K.P., Mateen S., Neves J., Garcia T.Y., Mooney S., Perdew G.H., Hubbard T.D., Lamba D.A, & Ramanathan A. (2016). A novel AhR ligand, 2AI, protects the retina from environmental stress. Scientific Reports, 6, 29025.
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4

Preparation and Characterization of Polychlorinated Biphenyl Standards

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2,2′-Dichlorobiphenyl (PCB 4), 2,4′-dichlorobiphenyl (PCB 8), 3,3′-dichlorobiphenyl (PCB 11), 2,2′,5-trichlorobiphenyl (PCB 18), 2,4′,5 -trichlorobiphenyl (PCB 31) and 2,2′,5-trichlorobiphenyl-4′-ol (4′-OH-PCB 18) were purchased from AccuStandard (New Haven, USA). 2,4,4′-Trichlorobiphenyl (PCB 28) was from Dr. Ehrenstorfer (LGC Standards, Teddington, UK). 2,4′-dichlorobiphenyl-4-ol (4-OH-PCB 8), 3,3′-dichlorobiphenyl-4-ol (4-OH-PCB 11) and 3,3′-dichlorobiphenyl-5-ol (5-OH-PCB 11) were synthesized as described in Supplementary Experimental Procedures. The purities of all LC-PCB congeners and trace impurities are reported in Supplementary Tables 1 and 2. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), 99% purity, was from Cambridge Isotope Laboratories (Andover, USA). ICI 182,780 was from Tocris Bioscience (Bristol, UK). Mifepristone was purchased from (Cayman Chemical). Cyproterone acetate (CPTA) was from Santa Cruz Biotechnology (Santa Cruz, USA). 17β-Estradiol (E2), 5α-dihydrotestosterone (DHT), triiodothyronine (T3), rosiglitazone (RGS), phenobarbital (PB) and rifampicin (RIF) were from Sigma-Aldrich (Prague, Czech Republic). All other chemicals not specified above were of the highest purity available; they were purchased from Sigma-Aldrich, if not indicated otherwise.
Pěnčíková K., Svržková L., Strapáčová S., Neča J., Bartoňková I., Dvořák Z., Hýžd’alová M., Pivnička J., Pálková L., Lehmler H.J., Li X., Vondráček J, & Machala M. (2018). In vitro profiling of toxic effects of prominent environmental lower-chlorinated PCB congeners linked with endocrine disruption and tumor promotion. Environmental pollution (Barking, Essex : 1987), 237, 473-486.
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