Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This method is similar to those described in Reference 25. The 96-well plate was then put into an iMark Microplate (BioRad) to measure optical densities (OD) at 490 nm. The cell morphology of A375 cells incubated with GNRs at different concentrations are shown in
Imark microplate
The IMark microplate is a laboratory equipment designed for absorbance measurements in microplate format. It is used to quantify the amount of light absorbed by samples in a microplate, which can be used to measure various biological and chemical reactions.
7 protocols using imark microplate
Photothermal Therapy of Melanoma Cells
Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This method is similar to those described in Reference 25. The 96-well plate was then put into an iMark Microplate (BioRad) to measure optical densities (OD) at 490 nm. The cell morphology of A375 cells incubated with GNRs at different concentrations are shown in
Ferric Reducing Antioxidant Power Assay
Cytotoxicity Evaluation of Nanomaterials
Evaluating NF-κB Activation by Bacterial Strains
Briefly, cells were washed with PBS, dissociated in PBS and collected in growth media. Cells were plated in 96-well plates at a density of 25,000 cells/well. To evaluate the effect of bacteria strains on LPS inducing NF-κB promoter activation, cells were treated with 10 ng/ml LPS in presence or absence of 10% supernatants and incubated in a CO2 incubator. Treatments proceeded for 22 h at 37°C and 5% CO2, after which the detection of Secreted Embryonic Alkaline Phosphatase (SEAP) activity from cell culture supernatant was performed using QUANTI-blue solution according to manufacturer’s instructions. Briefly, 20 μl of cell-free supernatant was collected and analyzed for the presence of SEAP by mixing with 200 μl of sterile-filtered QUANTI-Blue detection media. After 2 h incubation at 37°C, optical density was measured at 655 nm on a microplate reader (iMark microplate, Bio-Rad).
Quantification of Hepatic Lipid Droplets
Cytotoxicity Assay for Osmium Complex
Cell Proliferation Assay for DAM and DAM-NPs
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