To detect iNOS, a 1:1,500 dilution of the primary antibody (mouse anti-mouse iNOS [BD Transduction Laboratories]) in blocking buffer (2.5% dry milk, 0.1% Tween 20) was added to the tissue, and the mixture was incubated in a humidified chamber overnight at 4°C. Slides were then washed in phosphate-buffered saline (PBS) and then incubated with biotinylated secondary antibody for 20 min at room temperature, washed in PBS, and incubated with streptavidin-peroxidase complex (LSAB1 kit [Dako, Carpinteria, CA]) for 20 min at room temperature. The reaction was developed with a 0.024% diaminobenzidine solution (Dako) and counterstained with Mayer’s hematoxylin for 60 s.
Lsab1 kit
Manufactured by Agilent Technologies
The LSAB1 kit is a laboratory equipment product offered by Agilent Technologies. The core function of this kit is to provide a tool for performing specific laboratory analyses, without further interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lsab1 kit
1
Immunohistochemical Detection of iNOS
2
Immunohistochemical Analysis of Mouse Tissue
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