Pifithrin α pft α

Manufactured by Beyotime

Sourced in China

Pifithrin-α (PFT-α) is a small molecule compound that functions as an inhibitor of p53-mediated transcriptional activation. It is commonly used as a tool in cellular and molecular biology research.

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Lab products found in correlation

Bcl 2, by Abcam (1 mentions) Gapdh, by Abcam (1 mentions) Bcl2 associated x (bax), by Abcam (1 mentions) Cyclin b1, by Cell Signaling Technology (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Propidium iodide, by Beyotime (1 mentions) Fitc annexin 5 apoptosis detection kit, by BD (1 mentions) P21 waf1 cip1, by Cell Signaling Technology (1 mentions) Cdc25a, by Cell Signaling Technology (1 mentions) Phosphor cdc2, by Cell Signaling Technology (1 mentions) Revertra ace qpcr rt kit, by Qiagen (1 mentions) Necrostatin 1, by MedChemExpress (1 mentions) Mitoquinone mitoq, by MedChemExpress (1 mentions) Anti p53 sc 126, by Santa Cruz Biotechnology (1 mentions) Mdivi 1, by MedChemExpress (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Interferon γ ifn γ, by Beyotime (1 mentions) Dulbecco s phosphate buffered saline, by Thermo Fisher Scientific (1 mentions) Anti pd l1, by Proteintech (1 mentions) Anti gapdh, by Proteintech (1 mentions)

Spelling variants of this product

Pifithrin-α (6 citations) PFT-α (2 citations)

4 protocols using pifithrin α pft α

Actinomycin-Induced Lung Cancer Apoptosis

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Actinomycin V (>98%) and actinomycin D (>98%) were dissolved and their concentration adjusted by dimethyl sulfoxide (DMSO) when required while the control group was treated with DMSO only. MTT and DAPI were acquired from Sigma-Aldrich Crop. (St. Louis, MO, USA). Annexin V-FITC Apoptosis Detection Kit was obtained from BD Biosciences (San Jose, CA, USA). Phosphor-Cdc2, Cdc2, Cdc25A, Cyclin B1, p53 and p21^Waf1/Cip1 were supplied by Cell Signaling Technology (CST, Inc, Beverly, MA, USA). Bcl-2, Bax and GAPDH antibodies were supplied by Abcam, Inc. (Cambridge, MA, USA). Propidium iodide and Pifithrin-α (PFT-α) were purchased from Beyotime Institute of Biotechnology (Shanghai, China). RNeasy mini kit and Rever Tra Ace qPCR RT kit were purchased from QIAGEN (Hilden, Germany) and Toyobo (Osaka Prefecture, Japan), respectively.
Human non-small-cell lung carcinoma cell lines A549, NCI-H1299 (p53-deficient) and normal human bronchial epithelial cells BEAS-2B were obtained from the Shanghai Institute for Biological Sciences (SIBS), Chinese Academy of Sciences (Shanghai, China) and we cultured the cells according to the supplier’s instructions.

Lin S.Q., Jia F.J., Zhang C.Y., Liu F.Y., Ma J.H., Han Z., Xie W.D, & Li X. (2019). Actinomycin V Suppresses Human Non-Small-Cell Lung Carcinoma A549 Cells by Inducing G2/M Phase Arrest and Apoptosis via the p53-Dependent Pathway. Marine Drugs, 17(10), 572.

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Mitochondrial Regulation in p53 Inhibition

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The p53 inhibitor Pifithrin‐α (PFT‐α) was obtained from Beyotime Biotechnology and was dissolved in DMSO. Mdivi1, a compound that inhibits mitochondrial fission, was obtained from MedChemExpress. It was then added to the cell medium at a concentration of 10 μM for 12 h. The mitochondrial antioxidant Mitoquinone (MitoQ) was purchased from MedChemExpress (Shanghai, China) and dissolved in DMSO to a concentration of 50 nM. Necrostatin‐1 was acquired from MedChemExpress and dissolved in DMSO to a concentration of 40 μM. Anti‐DRP1 (8570), Anti‐OPA1 (80471), Anti‐MFN1 (14739), Anti‐BAX (5023), Anti‐BCL2 (15071) and Anti‐GAPDH (5174) were provided by Cell Signaling Technology. Anti‐p53 (sc‐126) was supplied by Santa Cruz Biotechnology Inc. Anti‐rabbit IgG (HRP‐conjugated) (ab6721) and anti‐mouse IgG (HRP‐conjugated) (ab6789) were obtained from Abcam.

Ding R., Wang Y., Xu L., Sang S., Wu G., Yang W., Zhang Y., Wang C., Qi A., Xie H., Liu Y., Dai A, & Jiao L. (2024). QiDongNing induces lung cancer cell apoptosis via triggering P53/DRP1‐mediated mitochondrial fission. Journal of Cellular and Molecular Medicine, 28(9), e18353.

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Inhibition of p53 in H9c2 Cells

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For inhibition of p53 in response to H₂, H9c2 cells were treated with Pifithrin-α (PFTα, S1816, Beyotime, China), which was a p53 inhibitor that dissolved in dimethyl sulfoxide (DMSO). Chu et al. (2006) (link) have reported that expression of p53 in H9c2 cells was inhibited after the cells were treated with 20 μM PFTα and doxorubicin for 8 h. However, we observed that after treatment with 20 μM PFTα and 10 μM VK₃, H9c2 cells all died 2 days later. Therefore, the final concentration of PFTα was 10 μM in the DMEM in this study. DMSO as a control was given the same volume to PFTα to H9c2 cells. After 16 h, the culture medium was changed to DMEM with 1% FBS, 1% penicillin–streptomycin and 10 μM PFTα and then the treatments of VK₃ and H₂ were continued.

Chi J., Li Z., Hong X., Zhao T., Bie Y., Zhang W., Yang J., Feng Z., Yu Z., Xu Q., Zhao L., Liu W., Gao Y., Yang H., Yang J., Liu J, & Yang W. (2018). Inhalation of Hydrogen Attenuates Progression of Chronic Heart Failure via Suppression of Oxidative Stress and P53 Related to Apoptosis Pathway in Rats. Frontiers in Physiology, 9, 1026.

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Realgar-Based Anticancer Protocol

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Highly purified realgar supplied by the Sanmenxia Yuhuangshan Pharmaceutical Co., Ltd was prepared from mined natural realgar. The purity of As₄S₄ in the realgar preparation was greater than 95.0%, confirmed by repeated X‐ray powder diffraction analyses. Realgar was dissolved in Dulbecco's phosphate‐buffered saline (DPBS) (Thermo Fisher Scientific) and sterilized by filtration. The content of As in DPBS solution was determined by inductively coupled plasma atomic emission spectrometry at the Instrumental Analysis Center of Shanghai Jiao Tong University (Shanghai, People's Republic of China). As₄S₄ stock solution of 766.36 μM was stored at 4°C. DDP was supplied by Qilu Pharmaceutical. Anti‐p53 (cat: 21891‐1‐AP), anti‐PD‐L1 (cat: 17952‐1‐AP), and anti‐GAPDH (cat: 10494‐1‐AP) antibodies were purchased from Proteintech. Pifithrin‐α (PFT‐α) (cat: S1816‐25 mg), methyl‐thiazolyl‐tetrazolium (MTT) (cat: ST316), interferon‐γ (IFN‐γ) (cat: P5664‐100 ug) and sodium dodecyl sulfate (SDS) powder (cat: ST626) were purchased from Beyotime Institute of Biotechnology. MTT powder was dissolved in DPBS at a concentration of 5 mg/ml. SDS powder was dissolved in water at aconcentration of 10%.

Tian W., Sun Y., Cheng Y., Ma X., Du W., Shi W, & Guo Q. (2021). Arsenic sulfide reverses cisplatin resistance in non‐small cell lung cancer in vitro and in vivo through targeting PD‐L1. Thoracic Cancer, 12(19), 2551-2563.

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