Ne 4000 programmable 2 channel syringe pump

NPs were generated using a FNP process (Figure 1A) as previously described [10 (link)]. Briefly, NPs were generated using a three-inlet, confined impinging jet (CIJ) FNP device. The first two inlet inputs contained distilled, deionized (DDI) water. The third inlet contained a solution of 5 mg/mL PEG_3K-b-PLGA_20K containing 90 mol% unlabeled PEG_3K-b-PLGA_20K (Polyscitech) and 10 mol% maleimide-PEG_3K-b-PLGA_20K (Polyscitech) dissolved in acetonitrile. NPs of the defined size were generated by modulating volumetric flow rates of the three inlets using a NE-4000 Programmable 2 Channel Syringe Pump (New Era Pump Systems). The NPs generated through the device were collected in a water bath such that ≤10% v/v was acetonitrile. The collected NPs were dialyzed in a 3.5-kDa dialysis membrane (Repligen) at 4 °C against 4 L of deionized water with dialysate changes every six hours.

For intrastriatal microinfusion of AAV, mice were anesthetized by intraperitoneal administration of ketamine‐xylazine mixture (120 and 8 mg/kg, respectively) and appropriately positioned in a stereotaxic frame (Kopf Instruments, CA, USA). Microinfusion was made into both hemispheres of the dorsal striatum at the following stereotaxic coordinates: AP, +0.7 mm; ML, ±1.9 mm from midline; and DV, −2.3 mm below the dura. The infusion was carried out by NE‐4000 Programmable 2 Channel Syringe Pump (New Era Pump Systems, NY, USA). The infusion volume was 0.2 μl, and the infusion rate was 0.1 μl/min. After infusion, the microneedle was held in position for 2 min.