Human il 17 quantikine elisa kit

Sera from sarcoidosis patients and healthy controls were centrifuged at 1400 rpm for 15 min after 30 min of clotting and were stored at -80°C, followed by detection of IL-17A using the Human IL-17 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN) according to the manufacture’s protocol.

Plasma and synovial fluid levels of IL-17 were measured using Human IL-17 Quantikine ELISA Kit (D1700, R&D Systems). All steps were completed according to the instructions of the kit. Plasma levels of IL-17 were normalized to pg/ml.

PHPT patients were investigated by dual-energy X-ray absorptiometry (DEXA) scanner for the measurement of segmental bone mineral density (BMD) using a Hologic densitometer. Participants were scanned in light clothing, while lying flat on their backs with arms at their sides.
Venous blood samples were collected after an overnight fasting in all patients. Serum calcium, ionized calcium, phosphate, creatinine, and total alkaline phosphatase were measured according to the routinely used laboratory kits. Serum PTH levels were determined by electrochemiluminescence on an Elecsys 2010 (Roche Diagnostics, Mannheim, Germany), and serum 25-hydroxyvitamin D (25OHD) was measured by a chemiluminescent assay (LIAISON® test, DiaSorin Inc., Stillwater, MN, USA). Plasma IL-17A levels were assayed by Human IL-17 Quantikine ELISA kit (R&D System, Cat. D1700; intra-assay CV 4.7%, interassay CV 8.4%, sensitivity <3.0 pg/ml, by considering an additional point to the low range of the standard curve); plasma OPG was determined by the Human Osteoprotegerin ELISA kit (BioVendor, Cat. RD194003200; intra-assay CV 4.9%, interassay CV 9.0%, sensitivity 0.03 pmol/l) and plasma soluble RANKL by the sRANKL (total) human ELISA kit (BioVendor, Cat. RD193004200R; intra-assay CV 11.5%, inter-assay CV 12.7%, sensitivity 0.4 pmol/l).