Nanozoomer xr digital whole slide scanner

To validate the presence of EBV, EBER-ISH staining was performed on all 5 μm FFPE cases by an automated staining system (Ventana BenchMark ULTRA, Roche diagnostic, Monza-Italy), as previously described [18 (link)]. All steps were performed inside the instrument, from deparaffinization to counterstaining with appropriate positive and negative controls included in each staining run. A control slide prepared from a paraffin-embedded tissue block containing EBV-positive metastatic nasopharyngeal carcinoma in a lymph node accompanied each hybridization run. The EBER-ISH stained sections were scanned and analysed by Hamamatsu NanoZoomer-XR digital whole slide scanner.

Single IHC staining was performed on 4 μm formalin-fixed paraffin-embedded tissue (FFPE) sections that were baked for 1 h at 60 °C. Slides were stained using the automated Ventana BenchMark ULTRA platform (Ventana, Roche diagnostic, Monza, Italy) and UltraView Universal DAB detection kit (Ventana, Monza, Italy). The sections were incubated with primary antibody for 1 hour using the mouse monoclonal antibody 15C4 against LRG1 [29 (link)] (dilution 1:100), the rabbit monoclonal EP373Y antibody against CD34 (Abcam, ab81289; dilution 1:100), and the mouse monoclonal antibody 1A4 against α-SMA (Abcam, ab7817; dilution 1:100). Immunohistochemistry (IHC) assay was carried out with appropriate positive and negative controls included in each staining run. The IHC stained sections were scanned and analyzed by a Hamamatsu NanoZoomer-XR digital whole slide scanner. Control eye specimens were resected from a patient with lacrimal sac tumor (male, 83 years) and from a patient with neuroblastoma (female, 3 years).