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Anti mouse cd24

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Anti-mouse CD24 is a lab equipment product used for the detection and analysis of the CD24 surface antigen on mouse cells. It serves as a tool for immunological research and cell characterization.

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Lab products found in correlation

Anti mouse siglecf clone e50 2440, by BD (1 mentions) Cytexpert, by Beckman Coulter (1 mentions) Anti mouse cd31, by BioLegend (1 mentions) Anti mouse sca1, by BioLegend (1 mentions) Clone 36 e cadherin, by BD (1 mentions) Anti mouse ecadherin, by BD (1 mentions) Anti mouse cd41, by BioLegend (1 mentions)

2 protocols using anti mouse cd24

1

Characterizing Lung Immune Cells by Flow Cytometry

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Single cell suspensions were prepared from dissected mouse lungs and stained using fluorochrome-conjugated antibodies56 (link) that recognize cell surface markers and the cytokines TNF and IFN-γ present intracellularly. Antibodies used were purchased from BioLegend (San Diego, CA, USA) unless indicated otherwise: anti-mouse Ly6G (clone IA8), anti-mouse CD45 (clone 30-F11, BD Biosciences, San Jose, CA, USA), anti-mouse CD11b (clone M1/70), anti-mouse CD11c (clone N418), anti-mouse Ly6C (clone AL-21 BD Biosciences), anti-mouse MHC Class II I-A/I-E (clone M5/114.15.2), anti-mouse Siglec-F (clone E50-2440, BD Biosciences), anti-mouse CD54 (clone 3E2), anti-mouse CD103 (clone M290), anti-mouse IFNγ (XMG1.2), anti-mouse TNF (MP6-XT22), anti-mouse CD64 (clone × 54–5/7.1), and anti-mouse CD24 (clone M1/69). After gating out debris and doublet cells, neutrophils were identified as single cells expressing both CD45 and Ly6G. Flow cytometry was performed using a Cytoflex flow cytometer (Beckman Coulter, Brea, CA, USA) and data were analyzed using CytExpert (Beckman Coulter) software.
Gomez J.C., Dang H., Kanke M., Hagan R.S., Mock J.R., Kelada S.N., Sethupathy P, & Doerschuk C.M. (2017). Predicted effects of observed changes in the mRNA and microRNA transcriptome of lung neutrophils during S. pneumoniae pneumonia in mice. Scientific Reports, 7, 11258.
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2

Endothelial Cell Marker Antibody Panel

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The antibodies used were as follows:
Anti-mouse Etv2 (Abcam, EPR5229(2); western blotting 1:100)
Anti-mouse Erg (Abcam, 9FY; western blotting 1:100)
Anti-mouse Fli1 (Abcam, ab15289; western blotting 1:100)
Anti-mouse CD31 (Biolegend clone 390; flow cytometry 1:2,000)
Anti-mouse VE-Cadherin (Biolegend clone BV13; flow cytometry 1:500)
Anti-mouse Vegfr2 (DC101; flow cytometry 1:500)
Anti-mouse CD62e (BD Biosceinces, clone 10E9.6; flow cytometry 1:500)
Anti-mouse Itgb1 (BD Biosciences, clone 18/CD29; flow cytometry 1:500)
Anti-mouse Meca32 (BD Biosciences, clone MECA-32; flow cytometry 1:500)
Anti-mouse Tie2 (Biolegend, clone Tek4; flow cytometry 1:500)
Anti-mouse ECadherin (BD Biosciences, Clone 36/E-Cadherin; flow cytometry 1:500)
Anti-mouse CD34 (BD Biosciences, Clone RAM34; flow cytometry 1:500)
Anti-mouse Vcam (BD Biosciences, Clone 429 MVCAM.A; flow cytometry 1:500)
Anti-mouse Sca1 (Biolegend, clone D7; flow cytometry 1:500)
Anti-mouse CD41 (Biolegend, clone MWReg30; flow cytometry 1:500)
Anti-mouse CD24 (BD Biosciences clone M1/69; flow cytometry 1:500)
Anti-mouse VE-Cadherin (R+D AF1002; IF 1:100)
Anti-mouse CD31 (Biocare, Clone Mec13.3; IF 1:100)
Uncropped western blotting images are shown in Supplementary Fig. 5.
Schachterle W., Badwe C.R., Palikuqi B., Kunar B., Ginsberg M., Lis R., Yokoyama M., Elemento O., Scandura J.M, & Rafii S. (2017). Sox17 drives functional engraftment of endothelium converted from non-vascular cells. Nature Communications, 8, 13963.
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