For all experiments other than HeLa cell imaging, images were obtained using TCS-SP5 laser scanning confocal microscopy (Leica) with a PL APO CS 40×/1.25 Oil lens (Leica) for PN imaging or a PL APO CS 63×/1.40 Oil lens (Leica) for imaging of S2 cells. For imaging of HeLa cells, TCS-SP8 laser scanning confocal microscopy (Leica) with a PL APO CS 63×/1.40 Oil lens was used. Fields of view were randomly selected and all experiments were repeated more than three times. For PN clones other than Figure 4 , more than 10 clones were imaged and representative images were shown in Figures. For PN clones of Figure 4 , S2 cells and HeLa cells, the exact number of samples imaged was shown in each Figure.
Pl apo cs 40 1.25 oil lens
Manufactured by Leica
The PL APO CS 40×/1.25 Oil lens is a high-performance microscope objective designed for Leica microscopes. It features a numerical aperture of 1.25 and a magnification of 40×, making it suitable for a variety of applications that require high-resolution imaging. The lens is optimized for use with oil immersion, which can enhance the optical performance and resolution of the microscope system.
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3 protocols using pl apo cs 40 1.25 oil lens
1
Confocal Microscopy Imaging Protocol
2
Confocal Imaging of Neuronal and Cellular Samples
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For all experiments, images were obtained using TCS-SP5 confocal laser scanning confocal microscopy (Leica) with a PL APO CS 40×/1.25 Oil lens (Leica) for PN and mushroom body neuron imaging or a PL APO CS 63x/1.40 Oil lens (Leica) for imaging S2 cells. Fields of view were randomly selected and each biologically independent experiment was repeated more than two times.
3
Confocal Microscopy Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For all experiments other than HeLa cell imaging, images were obtained using TCS-SP5 laser scanning confocal microscopy (Leica) with a PL APO CS 40×/1.25 Oil lens (Leica) for PN imaging or a PL APO CS 63×/1.40 Oil lens (Leica) for imaging of S2 cells. For imaging of HeLa cells, TCS-SP8 laser scanning confocal microscopy (Leica) with a PL APO CS 63×/1.40 Oil lens was used. Fields of view were randomly selected and all experiments were repeated more than three times. For PN clones other than Figure 4 , more than 10 clones were imaged and representative images were shown in Figures. For PN clones of Figure 4 , S2 cells and HeLa cells, the exact number of samples imaged was shown in each Figure.
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