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Pmx backbone

Manufactured by Cosmo Bio
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The PMX backbone is a laboratory equipment component designed for various scientific applications. It serves as a core structural element to support and integrate other lab equipment and accessories. The PMX backbone provides a stable and modular platform for assembling and configuring customized laboratory setups.

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Lab products found in correlation

Pcdna5 backbone, by Thermo Fisher Scientific (2 mentions) Dynabeads protein g immunoprecipitation system, by Thermo Fisher Scientific (2 mentions) Lipofectamine ltx, by Thermo Fisher Scientific (2 mentions) Protease inhibitor cocktail, by Merck Group (2 mentions)

2 protocols using pmx backbone

1

Immunoprecipitation of A20 and p62 Complexes

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Cells were stimulated with Pam3CSK4 (100 ng/ml) or zymosan (100 μg/ml) and lysed with RIPA buffer and NP-40 (for immunoprecipitation) containing protease inhibitor cocktail (P8340, Sigma). Phosphatase inhibitor cocktail II was added for p-IκB detection. To examine the association between A20 and p62, NIH-3T3 cells were co-transfected with expression vectors for A20 and p62 (pcDNA5-A20-V5/His and pMI-p62-HA, respectively) by using Lipofectamine LTX (Invitrogen), as recommended by the supplier. The expression vectors for A20 and p62 were made in our laboratory, as fusions of the V5/His tag in the pcDNA5 backbone (Invitrogen) and the HA tag in the pMX backbone (Cosmobio), respectively. Immunoprecipitation was carried out by using Dynabeads Protein G Immunoprecipitation system (Invitrogen) with specific antibodies, followed by immunoblotting with indicated antibodies as recommended by the supplier. Signal intensity of blotting was determined by densitometry using the NIH-Image J software (http://rsbweb.nih.gov/ij/). Full blot data are shown in Supplementary Fig. 18.
Kanayama M., Inoue M., Danzaki K., Hammer G., He Y.W, & Shinohara M.L. (2015). Autophagy enhances NFκB activity in specific tissue macrophages by sequestering A20 to boost antifungal immunity. Nature communications, 6, 5779.
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2

Immunoprecipitation of A20 and p62 Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stimulated with Pam3CSK4 (100 ng/ml) or zymosan (100 μg/ml) and lysed with RIPA buffer and NP-40 (for immunoprecipitation) containing protease inhibitor cocktail (P8340, Sigma). Phosphatase inhibitor cocktail II was added for p-IκB detection. To examine the association between A20 and p62, NIH-3T3 cells were co-transfected with expression vectors for A20 and p62 (pcDNA5-A20-V5/His and pMI-p62-HA, respectively) by using Lipofectamine LTX (Invitrogen), as recommended by the supplier. The expression vectors for A20 and p62 were made in our laboratory, as fusions of the V5/His tag in the pcDNA5 backbone (Invitrogen) and the HA tag in the pMX backbone (Cosmobio), respectively. Immunoprecipitation was carried out by using Dynabeads Protein G Immunoprecipitation system (Invitrogen) with specific antibodies, followed by immunoblotting with indicated antibodies as recommended by the supplier. Signal intensity of blotting was determined by densitometry using the NIH-Image J software (http://rsbweb.nih.gov/ij/). Full blot data are shown in Supplementary Fig. 18.
Kanayama M., Inoue M., Danzaki K., Hammer G., He Y.W, & Shinohara M.L. (2015). Autophagy enhances NFκB activity in specific tissue macrophages by sequestering A20 to boost antifungal immunity. Nature communications, 6, 5779.
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