Body weight and food intake were recorded weekly. After an overnight fast, glycemia was measured weekly using a drop of whole blood taken from the tail and an Accu-Chek Performa blood glucose meter (Roche, Mannheim, Germany). After 6 weeks of treatment, fat and lean mass were recorded by MRI (EchoMRI 3-in-1 instrument; Echo Medical Systems, Houston, TX, USA). Finally, animals were fasted overnight (16 h) and euthanized by cervical dislocation. Whole blood was harvested right after the death of the mice and a small portion was collected in an EDTA-treated tube and put on ice until analysis. Glycated hemoglobin (HbA1c) levels were measured in EDTA-treated whole blood with a mouse Hemoglobin A1c (HbA1c) Assay Kit (Crystal Chem, Elk Grove Village, IL, USA) by comparing absorbance at 700 nm in a spectrometer with Hba1c controls according to the instructions of the manufacturer.
Mouse hemoglobin a1c hba1c assay kit
Manufactured by Crystal Chem
Sourced in United States
The Mouse Hemoglobin A1c (HbA1c) Assay Kit is a laboratory equipment product designed to measure the level of glycated hemoglobin (HbA1c) in mouse samples. HbA1c is an important indicator of average blood glucose levels over a period of time. The kit provides the necessary reagents and protocols to perform this analysis.
Automatically generated - may contain errors
5 protocols using mouse hemoglobin a1c hba1c assay kit
1
Glycemia and Body Composition Analysis
2
Biochemical and Adipokine Analyses
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Blood was collected from cardiac puncture for blood chemistry and adipokine assays.
The enzyme activities of GOT and GPT, in addition to TCHO and TG levels were analyzed from serum samples using Fuji biochemical slides and a Fuji Dri-Chem 4000i analyzer (Fujifilm Cooperation, Taipei, Taiwan) in the Taiwan Mouse Clinic at Academia Sinica. Plasma levels of adiponectin, leptin, IL-6, and insulin were measured using mouse ELISA kits (Merck Millipore, Taipei, Taiwan). The plasma free fatty acid was measured using an ELISA kit (ab65341, Abcam, Cambridge, MA, USA), and blood HbA1c was measured using the mouse Hemoglobin A1c (HbA1c) Assay kit (Crystal Chem, Elk Grove Village, IL, USA).
The enzyme activities of GOT and GPT, in addition to TCHO and TG levels were analyzed from serum samples using Fuji biochemical slides and a Fuji Dri-Chem 4000i analyzer (Fujifilm Cooperation, Taipei, Taiwan) in the Taiwan Mouse Clinic at Academia Sinica. Plasma levels of adiponectin, leptin, IL-6, and insulin were measured using mouse ELISA kits (Merck Millipore, Taipei, Taiwan). The plasma free fatty acid was measured using an ELISA kit (ab65341, Abcam, Cambridge, MA, USA), and blood HbA1c was measured using the mouse Hemoglobin A1c (HbA1c) Assay kit (Crystal Chem, Elk Grove Village, IL, USA).
3
HbA1c Quantification in Mouse Blood
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Whole blood was collected from the tail vein. HbA1c was measured using a Mouse Hemoglobin A1c (HbA1c) Assay Kit (#83010, Crystal Chem, Downers Grove, IL, USA) for the quantitative determination of HbA1c in whole blood depending on manufacturer's instructions. Serum was isolated by centrifugation at 2,000 rpm for 10 min. Blood chemistry analysis was performed at the Pohang Center for Evaluation of Biomaterials, Pohang Technopark in Pohang, Korea.
4
Comprehensive Metabolic Profiling in Mice
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For blood chemistry and adipokine assays, blood was collected from cardiac puncture at the conclusion of experiments. The TCHO levels were analyzed from serum samples using Fuji biochemical slides and a Fuji Dri-Chem 4000i analyzer (Fujifilm Cooperation, Taipei, Taiwan) in the Taiwan Mouse Clinic at Academia Sinica. Plasma levels of adiponectin, leptin, IL-6, and insulin were measured using mouse ELISA kits (Merck Millipore, Taipei, Taiwan). The plasma free fatty acids were measured using an ELISA kit (ab65341, Abcam, Cambridge, MA, USA), and blood HbA1c was measured using the mouse Hemoglobin A1c (HbA1c) Assay Kit (Crystal Chem, Elk Grove Village, IL, USA).
5
Monitoring Glycemic Control in Mice
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Weekly blood glucose measurements were performed using the Contour XT glucose meter (Ascensia diabetes Care, NJ, USA). Blood was sampled from the saphenous vein. The first drop of blood was wiped away and the second was used for the blood glucose measurement. Measurements were taken at the same time of day (between 11am and 1pm). The hemoglobin A1c (HbA1c) percentage was measured in whole blood and is used to determine the degree to which hemoglobin in the erythrocytes has become glycated. Whole blood was collected in the same manner as described for the blood glucose measurement, and HbA1c% was measured using the Mouse Hemoglobin A1c (HbA1c) Assay Kit (Crystal Chem, IL, USA, catalog# 80310).
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