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2 protocols using heptanesulfonic acid

1

Dopaminergic Receptor Binding Assay

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Compounds used in this study were purchased from Sigma-Aldrich (St. Louis, MO) unless indicated otherwise: HEPES, sodium pyruvate, penicillin, streptomycin, 2-mercaptoethanol, D-glucose, bovine serum albumin (Merck Millipore, Darmstadt, Germany), glacial acetic acid, heptanesulfonic acid, methanol, glutathione, NaOH, EDTA, dimethyl sulfoxide, L-DOPA, dopamine hydrochloride, S-(−)-raclopride (+)-tartrate salt, R-(−)-deprenyl hydrochloride, pargyline hydrochloride, ascorbic acid, (S)-(−)-sulpiride (Tocris, Bristol, United Kingdom), (−)-quinpriole Hydrochloride (Tocris), R-2230 (link) (gift of Dr. Amy Newman, synthesized at NIDA, NIH, Baltimore, MD), ML32131 (link) (synthesized at NINDS, NIH, Bethesda, MD) and [3H]L-DOPA (Dihydroxyphenylalanine, L-3,4-[ring 2,5,6-3H]) (American Radiolabeled Chemicals, St. Louis, MO).
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2

Dopaminergic Receptor Binding Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Compounds used in this study were purchased from Sigma-Aldrich (St. Louis, MO) unless indicated otherwise: HEPES, sodium pyruvate, penicillin, streptomycin, 2-mercaptoethanol, D-glucose, bovine serum albumin (Merck Millipore, Darmstadt, Germany), glacial acetic acid, heptanesulfonic acid, methanol, glutathione, NaOH, EDTA, dimethyl sulfoxide, L-DOPA, dopamine hydrochloride, S-(−)-raclopride (+)-tartrate salt, R-(−)-deprenyl hydrochloride, pargyline hydrochloride, ascorbic acid, (S)-(−)-sulpiride (Tocris, Bristol, United Kingdom), (−)-quinpriole Hydrochloride (Tocris), R-2230 (link) (gift of Dr. Amy Newman, synthesized at NIDA, NIH, Baltimore, MD), ML32131 (link) (synthesized at NINDS, NIH, Bethesda, MD) and [3H]L-DOPA (Dihydroxyphenylalanine, L-3,4-[ring 2,5,6-3H]) (American Radiolabeled Chemicals, St. Louis, MO).
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