Cd19 apc clone hib19

Manufactured by BioLegend

CD19-APC (clone HIB19) is a fluorescently labeled monoclonal antibody that binds to the CD19 antigen, which is expressed on the surface of B cells. This product is intended for use in flow cytometry applications.

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CD19-APC (40 citations) CD19 (HIB19; (23 citations) CD19 (clone HIB19, (12 citations) CD19-APC (HIB19) (4 citations) APC-anti-human CD19 (clone HIB19, (3 citations) APC-Cy7-conjugated anti-human CD19 (clone HIB19; (2 citations)

3 protocols using cd19 apc clone hib19

Myeloid Cell Enumeration from Plasma

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To assess the number of myeloid cells from plasma incubation experiments, cells were stained with the following panel: CD3-APC (clone HIT3a), CD19-APC (clone HIB19), CD56-APC (clone 5.1H11), CD14-FITC (clone M5E2), CD15-AF700 (clone HI98), CD11b-PE-Cy7 (clone ICRF44), CD34-BV650 (clone 561), and CD38-PE/Cy5 (clone HIT2) (BioLegend). After staining, cells were resuspended in FACS buffer with 2% CountBright beads (Invitrogen) to allow determination of absolute counts during analysis. Flow cytometry data were acquired on a Cytoflex LX (Beckman Coulter) and analyzed using FlowJo v10.1.

Reyes M., Filbin M.R., Bhattacharyya R.P., Sonny A., Mehta A., Billman K., Kays K.R., Pinilla-Vera M., Benson M.E., Cosimi L.A., Hung D.T., Levy B.D., Villani A.C., Sade-Feldman M., Baron R.M., Goldberg M.B., Blainey P.C, & Hacohen N. (2021). Plasma from patients with bacterial sepsis or severe COVID-19 induces suppressive myeloid cell production from hematopoietic progenitors in vitro. Science Translational Medicine, 13(598), eabe9599.

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Multiparametric Flow Cytometry Analysis

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Single-cell suspensions from the BM and peripheral blood were first incubated with human BD Fc block (BD Biosciences) to block Fcγ receptor and then stained with the following anti-human Abs: CD19-APC (clone HIB19, BioLegend), CD34-PE (clone 563, BD Biosciences), CD20-FITC (clone 2H7, BioLegend), CD27-APC/Cyanine7 (clone O323, BioLegend), IgD-PE (clone IA6-2, BD Biosciences), IgM-FITC (clone G20-127, BD Biosciences), CD43-PE (clone CD43-10G7, BioLegend), CD38-PE/Cy7 (clone HIT2, BioLegend), CD38-Percp/Cy5.5 (clone HIT2, BD Biosciences), CD24-PE (clone ML5, BD Biosciences), CD27-V450 (clone M-T271, BD Biosciences), IgD-BV510 (clone IA6-2, BD Biosciences), IgG-PE/Cy7 (clone G18-145, BD Biosciences), Ki-67–PE (clone Ki-67, BioLegend), CD80-FITC (clone L307.4, BD Biosciences), CD86-PE (clone IT2.2, BD Biosciences), HLADR-PE (clone G46-6, BD Biosciences) and CD69-PE/Cy7 (clone FN50, BD Biosciences). 7-AAD Viability Staining Solution (eBioscience, Thermo Fisher Scientific) was used for live versus dead cell discrimination. Samples were analyzed with a FACSVerse flow cytometer (BD Biosciences) using the FACSuite software. Data analysis was performed with FlowJo 10 software (Treestar).

Min Q., Meng X., Zhou Q., Wang Y., Li Y., Lai N., Xiong E., Wang W., Yasuda S., Yu M., Zhang H., Sun J., Wang X, & Wang J.Y. (2021). RAG1 splicing mutation causes enhanced B cell differentiation and autoantibody production. JCI Insight, 6(19), e148887.

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Multiparametric Flow Cytometry of Spleen Cells

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Spleen samples were processed into single cell suspensions using the GentleMACS tissue dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). The cells were passed through a 100μm screen and centrifuged. Whole blood (collected in heparin) or spleen cell suspension were stained with the indicated antibodies. The stained samples were resuspended in 125 μL of 1X DPBS CMF for acquisition on the flow cytometer. Flow cytometric data acquisition was performed using the FACSCanto^™ or FACSCantoII^™ flow cytometer. Data was acquired using BD FACSDiva^™ software (version 8.0 or higher). Antibodies and reagents used here included: BioLegend (San Diego, CA) human CD45 BV510 clone HI30, mouse CD45 V421 clone 30-F11, CD19 APC clone HIB19, CD56 PE clone HCD56, CD14 APCCy7 clone M5E2, CD33 PECy7 clone P67.6, CD3 FITC clone UCHT1, CD4 PECy7 clone SK3, CD8 APC clone SK1, CD69 BV510 clone FN50, and 7AAD

Aparicio S., Morrison A., Gould A., Gilthorpe J., Chaudhuri C., Rigby P., Krumlauf R, & Brenner S. (1995). Detecting conserved regulatory elements with the model genome of the Japanese puffer fish, Fugu rubripes.. Proceedings of the National Academy of Sciences of the United States of America, 92(5), 1684-1688.

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