Small-interfering RNA (siRNA) targeting human KLF4 (si-KLF4) and a nonspecific siRNA (si-NS) were purchased from Santa Cruz Biotechnology. Transfection was performed using Lipofectamine™ reagent (Invitrogen) following the manufacturer's instructions. At 24 h following transfection, LX-2 cells were incubated with or without TNF-α (10 ng/mL). Cells were then harvested and used for RT-PCR and Western blotting assays.
Si ns
Manufactured by Santa Cruz Biotechnology
Si-NS is a laboratory equipment product manufactured by Santa Cruz Biotechnology. It is designed to provide researchers with a tool for specific applications. The core function of Si-NS is to assist in scientific investigations, but details on its intended use are not available.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using si ns
1
Knockdown of KLF4 in LX-2 Cells
2
Investigating KLF4 Regulation in VSMCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SiRNAs targeting rat KLF4 (si-KLF4) and nonspecific siRNAs (si-NS) were purchased from Santa Cruz Biotechnology. Transfection was performed using a Lipofectamine reagent (Invitrogen) according to the manufacturer’s instructions. At 24 h post-transfection, VSMCs were treated with or without PIO (20 μM). Cells were then harvested and used for qRT-PCR and Western blotting assays.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!